Biomedical subjects

J Llorente

Publications and source records attributed to J Llorente.

15 recordsLinked to original sources

PubMed2000

Gas chromatographic determination of acrinathrine and 3-phenoxybenzaldehyde residues in honey.

A procedure involving an extraction step and further gas chromatographic analysis with flame ionization detection to determine residues of acrinathrine and its main metabolite, 3-phenoxybenzaldehyde, in honey is proposed. Residues can be isolated from the matrix by means of liquid-liquid extraction with a mixture of benzene-isopropanol, by solid-phase extraction with octadecylsilane cartridges or Florisil packed columns, the latter method giving higher recoveries. Assays on spiked honey samples are carried out to test the procedures that are afterwards applied to honey samples from treated beehives.

Benzaldehydes↗

PubMed2000

Determination of rotenone residues in raw honey by solid-phase extraction and high-performance liquid chromatography.

A method for determining residues of the insecticide rotenone in raw-honey by high-performance liquid chromatography (HPLC) is described. To extract the residues, organic solvents such as ethyl acetate, n-hexane/dichloromethane and solid-phase extraction with octadecylsilane cartridges or Florisil packed columns were tested. Determination was carried out by reversed-phase HPLC using acetonitrile-buffer phosphate (pH 7) (60:40, v/v) as mobile phase and detection at 210 nm. Although the data showed that the two extraction methods were able to isolate the pesticide residues, the extraction on octadecylsilane cartridges was preferred due to its simplicity and higher recovery. Recoveries depended strongly on the fortification level for the two extraction procedures. Practical determination limits of 0.015 mg/kg were obtained. In the analysis of honeys, from beehives treated with rotenone at therapeutical doses for 1 month, residual amounts below 0.2 mg/kg were found.

Chromatography, High Pressure Liquid↗

PubMed1999

[Use of double PCR in an experimental mode of HSV-1 infection of the facial nerve].

Recent studies suggested herpes simplex virus type 1 as a major etiologic factor in Bell's palsy. To analyze different aspects of facial nene infections, Swiss mice were inoculated with HSV-l in tongue (41 animals) and auricle (44). Nineteen mice developed unequivocal signs of nevous infection, but only in 1 mouse was evident a facial palsy. Mice were sacrificed at different intervals from inoculation, and facial nerves, Gasser ganglia and brain stem were obtained to test the presence of HSV-1 by nested PCR and viral culture. Virus was detected in the 3 types of samples, but identification was more frequent in animals injected in tongue and sacrificed during the acute infection. Nested PCR was far more sensitive than culture, particularly during viral latency. According with our results, HSV-1 could origin facial palsy, but is possible that lesions are immunomediated and localized at brain stem.

Acute Disease↗

PubMed1997

A clinical algorithm for the management of abnormal mammograms. A community hospital's experience.

Mammography is an important tool in the early detection of breast cancer, but its use has been criticized for stimulating the performance of unnecessary breast biopsies. We retrospectively reviewed the results of breast biopsies preceded by abnormal mammograms at a community hospital for three 5-month periods--baseline, postintervention, and follow-up--to determine the effectiveness of algorithm-based care for patients with an abnormal mammogram. Cases in which there was a definite or implied recommendation for biopsy by a radiologist revealed a baseline positive predictive value of 4% (2/45), a postintervention positive predictive value of 21% (9/42), and a follow-up phase positive predictive value of 18% (5/28). A Fisher's exact test of the preintervention and postintervention positive predictive values after an abnormal mammogram with a "recommendation for biopsy" was significant (n = 87, P = .023). A Kruskal-Wallis analysis of variance to determine if there had been an increase in the mean lesion size of breast cancers detected over the 3 study periods was not significant. The results of this study suggest that developing a clinical algorithm under the leadership of an opinion leader combined with continuing medical education efforts may be efficacious in reducing the incidence of unnecessary surgical procedures.

Algorithms↗

PubMed1995

[Esophagogastroplasty in childhood: experience in 4 patients].

The most common indications of esophageal replacement in childhood are the esophageal atresia with long gap and caustic strictures. Several techniques has been described, using different components of the digestive tract. Total gastric transposition was performed in 4 children. The indications were esophageal atresia without tracheoesophageal fistula in 3 cases and a caustic stricture in other patient. Follow-up was 12 to 32 months. All patients are normally feed. One patient presented an anastomotic leak that closed spontaneously with total parenteral nutrition but ultimately led to an anastomotic stricture requiring pneumatic dilatation. Other patient presented stricture by sternal compression and required surgical repair. Total gastric transposition is a easy and physiologic technique, with low morbidity and good functional results.

Age Factors↗

PubMed1994

Laparoscopic gastric resection for gastric leiomyoma.

Less than 2% of gastric neoplasms that are resected surgically are of smooth-muscle origin. Gastric leiomyomas are not encapsulated, and the distinction of benign from malignant leiomyoma may be difficult. Some of these tumors manifest malignant behavior. The histological basis for the diagnosis of benign or malignant smooth-muscle tumor is not entirely satisfactory, and misclassification occurs in some cases. The aggressiveness of those tumors reported as malignant is usually low, and the term "malignant leiomyoma" is usually used rather than the more ominous "leiomyosarcoma." A case is presented of a patient with a 4.5-cm leiomyoma of the gastric antrum resected by the laparoscopic approach. Four laparoscopy trocars were used and multiple applications of the Endo-GIA were needed. Satisfactory margins of resection were obtained. The patient made an excellent recovery with minimal pain. She promptly returned to work and full physical activity.

Adult↗

PubMed1992

Laparoscopic cholecystectomy in the ambulatory surgery setting.

Laparoscopic cholecystectomy is now a well-established alternative to open cholecystectomy. It is less invasive and recovery generally is quicker and less painful. Several articles have been published in support of outpatient laparoscopic cholecystectomy. The main benefits are cost savings and patient and surgeon convenience. The main obstacles, however, are patient reluctance and surgeons' concern about sending a patient home without benefit of professional supervision after abdominal surgery and a few hours of general anesthesia. A new approach, consisting of surgery in an Ambulatory Surgery Center (ASC) and overnight observation in a free-standing Post Surgery Recovery Center (PSRC), is presented; and the first 100 patients in the study are reported. Laparoscopic cholecystectomy in the ASC with overnight observation in the PSRC offers the advantages of outpatient surgery, namely, lower cost and greater patient and surgeon satisfaction, while allowing the safety and convenience of remaining under professional supervision for the first 24 hours.

Adolescent↗

PubMed1991

Laparoscopic bilateral orchiectomy for testicular feminization syndrome.

A 13-year-old female patient with complete androgen insensitivity (testicular feminization syndrome) had an excision of bilateral intra-abdominal testicles via the laparoscopic approach. The patient had a very smooth, fast recovery from her surgery. The laparoscopic approach should be considered for most patients in need of surgical removal of undescended or intra-abdominal testes.

Adolescent↗

PubMed1990