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Biomedical subjects

J Losy

Publications and source records attributed to J Losy.

11 recordsLinked to original sources
PubMed1990

Detection of myelin basic protein-like material in cerebrospinal fluid of multiple sclerosis patients by immunoblot assay.

Myelin basic protein (MBP)-like material in 15 cerebrospinal fluid (CSF) samples from patients with multiple sclerosis (MS) was analyzed by isoelectric focusing (IEF) followed by immunoblot assay using rabbit antiserum against human MBP- peptide 69-89, which contains the dominant epitope for MBP-like material. Samples from seven of 10 MS patients with disease in the exacerbation stage showed one band and in three other samples, a number of faint bands also appeared in the alkaline pH region in addition to the one band. CSF from five MS patients whose disease was in remission showed no detectable bands. Our results are consistent with those obtained by quantitative assay, reported in the literature.

Amino Acid Sequence↗
PubMed1990

Identification of IgG subclasses' oligoclonal bands in multiple sclerosis CSF.

IgG subclasses' oligoclonal bands in unconcentrated CSF from MS patients were detected by isoelectric focusing in agarose gel with subsequent immunoblotting using mouse monoclonal antibodies to human IgG subclasses and double-antibody avidin-biotin-alkaline phosphatase system. All MS CSF showed presence of oligoclonal bands specific to the IgG1 subclass; in addition, several of these samples also had oligoclonal bands specific to IgG3, IgG2, or IgG4, in order of decreasing frequency. Since the CSF of a greater number of MS patients showed oligoclonal bands specific to the IgG1 and IgG3 subclasses, the findings are consistent with those reported in patients with chronic viral infections and autoimmune diseases.

Humans↗
PubMed1989

[A case of subdural abscess as a complication of suppurative meningitis].

Subdural empyema is a rare intracranial complication of life-threatening importance. A case of this empyema was observed in a patient aged 29 years during purulent meningitis. The rarity of this disease may cause difficulties in differential diagnosis against more frequently occurring cerebral abscesses. Early diagnosis, in which computerized tomography is particularly helpful, makes possible early neurosurgical treatment which is of decisive significance for the survival.

Adult↗
PubMed1989

Distribution of leukosialin (W3/13)-like immunoreactivity in the rat central nervous system.

The monoclonal antibody W3/13, which is directed against leukosialin (a molecule present on certain types of leukocytes), was found to react immunohistochemically with distinct regions and structures in the rat CNS. The leukosialin (W3/13)-like immunoreactivity occurred on neurite-like fibres and on bouton-like structures on nerve cell bodies and their dendrites. This labelling was most pronounced in certain subcortical areas and brain stem nuclei including different thalamic nuclei, olfactory tubercle, superior olive, cochlear and vestibular nuclei, motor and somatosensory nuclei. In the cerebellum, immunoreactive fibres were prominent in the molecular layer. In the spinal cord there was a dense labelling of fibres in the whole grey matter.

Animals↗
PubMedDate not supplied

[IgG1-IgG4 subclasses in the cerebrospinal fluid and blood serum and their synthesis in the central nervous system in multiple sclerosis].

IgG1, IgG2, IgG3 and IgG4 subclasses were detected in cerebrospinal fluid and sera from 32 patients with MS. The CSF levels of IgG1 and IgG3 subclasses in MS patients were significantly higher than those of controls. There were no statistical differences between the values of IgG subclasses in MS and control sera. The IgG1 and IgG3 indices were significantly higher in MS patients than in controls showing that among four IgG subclasses IgG1 ang IgG3 were synthesized in the central nervous system of patients with MS. The elevation of IgG1 and IgG3 indices in MS patients was found more frequently (in about 90% of patients) than the elevation of the general IgG index (in 72% of patients).

Antibodies, Monoclonal↗
PubMedDate not supplied

[Multiple sclerosis--studies of the correlations between selected parameters of immune response and the clinical course of the disease].

In the paper the following immune response parameters were analyzed: number of leucocytes in the cerebrospinal fluid, IgG index, presence and number of oligoclonal IgG bands. The correlation between these parameters and between the parameters and clinical course of multiple sclerosis was studied. Oligoclonal bands were present in the cerebrospinal fluid in 92% cases of MS, and that was the most frequent abnormality among immune response parameters studied. There was a correlation between IgG index, number of leucocytes in the cerebrospinal fluid and number of oligoclonal IgG bands in patients with relapsing-remitting course of the disease. We did not find any statistical differences between the number of leucocytes in the cerebrospinal fluid, the frequency of pleocytosis and the type of the disease, relapsing remitting or chronic progressive. The type of the disease, its duration and number of relapses did not significantly influence the IgG index values and number of IgG oligoclonal bands in the cerebrospinal fluid.

Adult↗
PubMedDate not supplied

[Circulating immune complexes in multiple sclerosis].

Circulating immunocomplexes were detected in sera from MS patients using solid-phase ELISA anti-C3 method. Estimations have revealed positive results in 71% of 21 MS cases. They were positive in 73% of MS cases with relapse-remitting course and in 66% of MS cases with chronic-progressive course compared with 5% among controls. The concentration of immunocomplexes in serum was 62 +/- 4 micrograms/ml in patients with chronic-progressive course and was significantly higher than the concentration of immunocomplexes in patients with relapse-remitting course of the disease--40.1 +/- 5.1 micrograms/ml.

Antigen-Antibody Complex↗
PubMedDate not supplied

[Oligoclonal antibodies in the diagnosis of multiple sclerosis and other diseases of the nervous system].

The detection of CSF oligoclonal IgG is one of the most useful laboratory tests to aid in the diagnosis of multiple sclerosis. Oligoclonal IgG is synthesized within the CNS and is usually detected in the CSF by agar and agarose gel electrophoresis or by isoelectric focusing. Oligoclonal IgG is present in about 90-95% in patients with clinically definite MS. There is a predominance of kappa light chains and IgG1 and IgG3 subclasses in oligoclonal IgG in MS. The specificity of oligoclonal IgG in this disease is still unknown, as antibodies, for instance against measles virus or myelin basic protein, represent only a minimal part of intrathecally synthesized IgG.

Clone Cells↗
PubMedDate not supplied

[Anti-GFAP antibodies in the cerebrospinal fluid of patients with multiple sclerosis and other neurologic diseases].

The antibodies against glial fibrillary acidic protein (GFAP) in the CSF of patients with multiple sclerosis and other neurological diseases were determined. It was shown that the binding activity of CSF IgG with GFAP was higher in patients with MS and other organic neurological diseases as compared to control group (neurotics). The results support the idea, that in SM there is a general immune dysregulation, leading to production of a variety of autoantibodies against different antigens, including the presently shown GFAP.

Autoantibodies↗
PubMedDate not supplied

Catecholamines in the rat brain during hypoglycemic convulsions and coma.

The purpose of the study was to investigate the relation between the catecholamines: noradrenaline and dopamine in the rat brain on one hand and hypoglycemic convulsions and coma on the other. Concentrations of noradrenaline in the hypothalamus, brain stem and cerebral cortex were decreased during hypoglycemic convulsions and were lower during coma than those during convulsions. Dopamine concentration in the striatum was decreased during convulsions and coma. It was shown that the decrease in concentration of catecholamines was a result of hypoglycemia but not of insulin action itself. Clonidine- alpha 2 agonist accelerated occurrence and prolonged duration of hypoglycemic convulsions. Haloperidol-dopamine receptor blocker had no effect on the time of occurrence or duration of convulsions and coma. The results indicate that noradrenaline may exert an inhibitory influence on hypoglycemic convulsions. No evidence has been provided to support involvement of dopamine in the control of hypoglycemic convulsions and coma.

Animals↗