[Serum creatine kinase in patients treated with depot neuroleptics. A pilot study].
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Adolescent↗
Biomedical subjects
J Lyngbye
Publications and source records attributed to J Lyngbye.
Pregnancy-specific beta 1-glycoprotein (SP1) determined by means of electroimmunoassay, radial immunodiffusion and nephelometry.
A comparison has been made between rocket-immunoelectrophoresis (RIE), radial immunodiffusion (RID) and automated immunonephelometry (AIP) in the assay of pregnancy-specific beta 1-glycoprotein (SP1) in serum from pregnant women. Using RIE an interaction was demonstrated between the various SP1-reactive molecular populations causing a bias of up to 10%. An interaction corresponding to this phenomenon cannot be demonstrated when using RID and AIP. When correlating the serum-SP1 concentration of samples containing various ratios of SP1-reactive molecules by means of RIE, RID and AIP, it was demonstrated that there was no correlation between the results achieved using one method compared to the results achieved by either of the other methods. The results achieved using one method can therefore exclusively be judged from reference values determined using the same method. The analysis time is essentially shorter with AIP than with RIE and RID.
Female↗
Change in binding properties of folate-binding protein in cow's whey due to removal of a cofactor during affinity chromatographic purification.
The folate-binding protein in cow's whey was purified by affinity chromatography on folate or methotrexate-AH-Sepharose 4B. A change in basic binding properties of the protein occurred probably due to the fact that material removed during affinity chromatography contains a cofactor of great importance to folate binding.
Animals↗
Determination of alkaline phosphatase (EC 3.1.3.1) on the SMAC using diethanolamine in a modified manifold.
The SMAC method for alkaline phosphatase was modified inorder to determine the enzyme activity according to the Scandinavian recommended method [1]. The method is not strictly 'Scandinavian', but the correlation with the strict Scandinavian method performed on the LKB 8600 Reaction rate analyzer was excellent. The modified method was linear up to at least 2000 U/l.
Alkaline Phosphatase↗
Early diagnosis of acute myocardial infarction by means of aspartate aminotransferase, creatine kinase and creatine kinase isoenzyme MB.
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Adult↗
Cooperative binding of folate to a protein isolated from cow's whey.
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Animals↗
Effect of methotrexate on folate binding to a folate binding protein in cow's milk.
Folate in cow's milk was strongly bound to a minor whey protein. Methotrexate inhibited the folate binding in a log dosis dependent manner, but was a rather weak inhibitor. The binding mechanism of folate changed from a cooperative to a non-cooperative type in the presence of methotrexate. This model system could be used in investigations on human body fluids and tissues.
Animals↗
Difference between free and ligand-bound folate-binding protein in cow's milk concerning affinity for chromatographic gels.
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Animals↗
Mechanism of folate binding in milk.
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Animals↗
Folate binding by human milk protein.
Human whey was subjected to gel chromatography. Two folate binding protein fractions wer eluted: a major one (Mr congruent to 30,000) and a minor one (Mr greater than 200,000). The former folate binding fraction (FB) could be isolated from whey by means of ion exchange chromatography. Equilibrium dialysis experiments with whey and isolated FB indicated the existence of cooperatively interacting sites in folate binding. The folate analogue methotrexate inhibited folate binding. Inhibition was apparently of a competitive type since methotrexate did not reduce maximum binding capacity. Cooperativity was lost in the presence of methotrexate. Binding affinity was inversely proportional to the concentration of FB suggesting involvement of a polymerizing protein system in binding. None of the other fractions eluted on the ion exchange column could bind folate.
Binding Sites↗
Diagnostic value of routine liver tests.
In 131 patients on a medical service and 97 patients on a surgical service, in whom a diagnosis of hepatobiliary disease was verified in the hospital, the diagnostic value of routine liver tests performed soon after admission was evaluated by stepwise discriminant analysis. By measurements of alanine aminotransferase, alkaline phosphatases, gamma globulin, prothrombin time, bilirubin, and albumin, half of the medical patients were correctly classified into one of seven diagnostic categories. Aminotransferase contributed most to the classification, being twice as effective as random allocation. Decreasing the number of diagnostic categories to three (hepatitis, fatty liver, and chronic liver disease) increased the frequency of correct allocation to 80%. The allocation of all the patients to seven medical and four surgical diagnostic categories by means of four tests (aminotransferase, alkaline phosphatases, prothrombin time, and bilirubin) was significantly improved by each step with a misclassification rate of 55% when all tests were used. A reduction of the diagnostic groups to five (hepatitis, fatty liver, chronic liver disease, duct obstruction and tumor) increased the frequency of correct allocation to 63%. The analysis demonstrates the limited diagnostic effectiveness of routine liver tests when used alone. The absolute discrimination values depend on the a priori frequencies of the diagnostic groups investigated, and therefore may vary from time to time and from place to place.
Alanine Transaminase↗