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Biomedical subjects

J M Barry

Publications and source records attributed to J M Barry.

208 records · Page 12Linked to original sources
PubMed1966

Stimulation by insulin of the formation of ribonucleic acid and protein by mammary tissues in vitro.

1. Insulin is one of the hormones that are essential for successful tissue culture of explants of the mammary glands of pregnant mice. We report here effects of insulin on RNA and protein formation by mammary tissue from pregnant mice and rats incubated in tissue-culture medium 199. 2. The incorporation of [(14)C]adenine over 3hr. into the RNA of explants of the mammary glands of pregnant mice was increased by an average of 68% when the medium contained 5mug. of insulin/ml. Under similar conditions the incorporation into the RNA of slices of the glands of pregnant rats was increased by an average of 61%. Incorporation into the RNA of slices from lactating rats was stimulated to a smaller extent. 3. Adipose tissue was separated from the glands of pregnant mice and the effect of insulin on the incorporation of adenine into its RNA was studied. In whole explants the incorporation of adenine, both with and without insulin, is almost entirely into the RNA of the mammary parenchyma and not of the adipose tissue. 4. Insulin also stimulated by 38% the incorporation of [(14)C]leucine over 3hr. into the proteins of slices of the glands of pregnant rats. It had no significant effect on slices from lactating rats. 5. Actinomycin D (10mug./ml.) decreased the incorporation of [(14)C]adenine into the RNA of slices of the glands of pregnant rats by an average of 97%. Though it also decreased the incorporation of [(14)C]leucine into the proteins by an average of 25%, the percentage stimulation by insulin of this incorporation remained unchanged.

Animals↗
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8 weeks and wheezing.

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Adaptation, Psychological↗
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Current dental concepts in antibiotic prophylaxis for total joint replacement patients.

The subject of antibiotic prophylaxis has evoked controversy and much debate over the validity of such practice. In July 1997, the American Dental Association and American Academy of Orthopedic Surgeons issued an advisory statement concerning the use of antibiotic prophylaxis for patients with total joint replacements. A historical perspective of the subject is presented, with a review of the recommendation for total joint replacement antibiotic prophylaxis, a discussion of the new decision-making responsibility for the dentist, and medicolegal ramifications of this change.

Adult↗