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J M Boutry

Publications and source records attributed to J M Boutry.

5 recordsLinked to original sources
PubMed1979

The early outgrowth of catecholaminergic fibers and the development of the nucleus of the locus coeruleus in tissue culture of newborn mice in Leighton tubes.

A simple and reliable method of sampling explants from the nucleus of the Locus Coeruleus of newborn mice and their cultivation in Leighton tubes is described. This technique reliably provides cultures available for experiments lasting, at least, ten days. The cells of the nucleus of the Locus Coeruleus and newly formed norepinephrine-containing fibers are demonstrated by fluorescence histochemistry together with pharmacological tests. Their morphological characteristics before and after fixation and staining are described. Electron microscopy confirmed the healthy condition of these cultures.

Animals↗
PubMed1978

[Simple technic for culture of locus coeruleus nucleus in the newborn mouse].

The dissecting procedure and tissue culture technique on collagen coated coverslips in Leighton tubes, of newborn Mouse nucleus locus coeruleus and the regular histofluorescence demonstration of catecholaminergic fibers in the outgrowth zone are described. This simple method allows 10 day--long experiments .

Adrenergic Fibers↗
PubMed1976

Studies on autoimmune encephalomyelitis in the guinea pig. II. An in vitro investigation on the nature, properties, and specificity of the serum-demyelinating factor.

Complement-dependent demyelinating activity of whole brain homogenate (WBH)-induced experimental allergic encephalomyelitis (EAE) sera was tested on long term tissue cultures of in vitro myelinated fetal guinea pig cerebellum. Complement-fixing (CF) auto-antibodies were shown to be the responsible agents, as demonstrated in experiments where all reagents belonged to the same species: guinea pigs of outbred (Hartley) and even of inbred (S2 or S13) strains. These antibodies were of the IgG2 class as shown by Sephadex G-200 and DEAE cellulose fractionation experiments. The corresponding auto-antigen was present in the homogenate and myelin of the central nervous system (CNS) tissue. It was different from the encephalitogenic basic protein of CNS myelin (BP), as shown in experiments where the demyelinating auto-antibodies were induced, detected, and absorbed by WBH or by CNS myelin but not by BP. They were neither induced by nor cross-reacting with cerebroside and peripheral nervous system (PNS) tissue.

Animals↗
PubMed1975

Cytochemical studies on golgi apparatus, GERL, and lysosomes in neurons of dorsal root ganglia in mice.

Cytochemically demonstrable thiamine pyrophosphatase activity is present in the innermost Golgi element in both small and large neurons of the dorsal root ganglia in CF1, C57 black, and C57 beige mice, thus resembling the neurons of rat dorsal root ganglia. The localization of acid phosphatase (EC 3.1.3.2) activity in the large neurons of dorsal root ganglia in these mice is also similar to that in rats; it is not demonstrable in Golgi elements but is present in GERL and in three types of lysosomes apparently derived from GERL. However, the small neurons of the mouse differ from those of the rat in showing acid phosphatase activity in all elements of the Golgi apparatus. In the mouse neurons the acid phosphatase activity of residual bodies is "latent," i.e., it is not demonstrable in well-preserved cells.

Acid Phosphatase↗