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J M Coll

Publications and source records attributed to J M Coll.

60 records · Page 4Linked to original sources
PubMed1987

Injection of physiological saline facilitates recovery of ascitic fluids for monoclonal antibody production.

The intraperitoneal injection of physiological saline into anti-C-reactive protein (anti-CRP) hybridoma tumor-bearing mice facilitates extraction of diluted ascitic fluids. The dilution with physiological saline before withdrawal of ascitic fluid reduces the processing time in large scale operations, facilitates further manipulations, increases the number of extractions per animal and permits the recovery of ascitic fluid containing monoclonal antibodies from solid tumor-bearing mice.

Animals↗
PubMed1987

Heme increases peroxidase-antibody activity in aged conjugates.

Decay of activity in antibody-peroxidase conjugates is faster when highly diluted as in clinical kits. Following aging at 4 degrees C, some of this loss of activity can be restored by incubation with heme just before use. The addition of heme in the conjugate storage buffer prevents some decay of activity and is not due to the pseudoperoxidase activity of heme. This procedure permits conjugates to be presented in an easy ready-to-use form for clinical immunoenzymatic assays.

Antibodies↗
PubMed1987

Preliminary clinical studies of C-reactive protein quantified by enzyme-linked immunoassay.

We have used an enzyme-linked immunoassay technique to quantify human C-reactive protein (CRP). In this assay phosphorylethanolamine is covalently linked to polystyrene wells. Serum or plasma specimens are diluted 961-fold and assayed. After Ca2+-dependent binding of CRP to the plates, the complex is reacted with peroxidase-labeled anti-CRP antibody. The response varies linearly with CRP concentrations between 10 and 160 mg/L; the detection limit is 0.34 ng per well. The results correlate well (r greater than or equal to 0.90) with those of rate nephelometry. This new method can be automated, is not subject to interferences or cross reactivity, is highly reproducible, has low background values, and can be carried out at room temperature.

C-Reactive Protein↗
PubMed1977

Metabolically labeled cell membrane proteins in spontaneously and in SV40 virus transformed mouse fibroblasts.

A family of mouse fibroblast cell lines in exponential phase of growth were compared in protein constitution of their cell membranes. In preparations from these cells enriched in cell-surface membrane we observed one protein component (apparent molecular weight about 250 000) consistently to be reduced or absent in an SV40 virus transformed cell line, when compared with the normal cell line. No such compositional difference was observed in a spontaneously transformed tumorigenic clonal derivative cell line, or in subclones of such a derivative cell line, with or without SV40 virus infection. However, in metabolic labeling experiments with 14C-labeled mixed amino acids, a consistent decrease also was demonstrated in the biosynthesis of the same protein in the SV40 virus infected subclone, as compared to an uninfected sister subclone, during exponential growth. This specific difference in biosynthesis is apparently related to the presence and functioning of the SV40 gene, and correlates with the ability of these cells to grow in viscous medium, but not with cellular tumorigenicity.

Animals↗
PubMedDate not supplied

Application of inducible and targeted gene strategies to produce transgenic fish: a review.

Compared to mammals, fishes offer easier transgenic technology because each female produces hundreds of eggs, the manipulated embryos do not need to be incubated inside the mother, and the probability of their harboring human-related pathogens is lower. In the last 15 years, traditional methods using injections of fertilized fish eggs and strong viral promoters have resulted in the generation of many transgenic fish species; however, they showed random genome integration with some mosaicism and episomic expression. The use of inducible gene systems that control temporal and tissue expression and of gene-targeting methodologies based on homologous recombination is desirable to control the expression, efficiency of insertion, and locus of incorporation of transgenes into fish genomes. A variety of systems developed for mammals are now available to be tested in fishes. The use of such systems would require further development of stem cell or nuclear transplant technologies in fish. Most of that work remains to be explored.

Animals↗