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J M Curtis

Publications and source records attributed to J M Curtis.

At least 55 records · Page 3Linked to original sources
PubMed1981

Resistance to cutaneous leishmaniasis in genetically susceptible BALB/c mice.

When injected cutaneously with promastigotes of an isolate of Leishmania tropica, BALB/c mice develop progressive cutaneous disease whereas lesions in BALB/c.H-2k mice heal after several weeks. Lesions in BALB/c mice injected into deep subcutaneous tissues with promastigotes are less obvious early but much more prominent later than in mice after strict intradermal injection. BALB/c mice injected with Corynebacterium parvum together with a preparation of frozen and thawed infected macrophages are more resistant to cutaneous disease than mice injected with either adjuvant or crude antigen mixture alone. Results of these experiments, and those on other mouse strains reported previously, will aid in the choice of mouse and injection regime to be used in testing the efficacy of isolated L. tropica antigens as vaccines.

Animals↗
PubMed1980

Cutaneous leishmaniasis in mice: disease patterns in reconstituted nude mice of several genotypes infected with Leishmania tropica.

BALB/c mice differ from CBA/H, (CBA/H x BALB/c)F1 and C57BL/6 mice in being highly susceptible to infection with a particular isolate of the intramacrophage protozoan parasite, Leishmania tropica. This is true over a wide range of challenge doses of promastigotes administered intradermally although, at low doses, male BALB/c are able to restrict the rate at which cutaneous lesions increase in size. Resistance to infection in highly susceptible hypothymic nude (nu/nu) mice of CBA/H and C57BL/6 genotypes can be achieved readily with as few as 10(6) syngeneic lymphoid cells. In CBA/H.nu/nu mice, Lyl+2- cells in low dose cellular inocula appear to be involved in restoring resistance (i.e. rapid resolution of infection). Surprisingly, a proportion of BALB/c.nu/nu mice injected with syngeneic lymphoid cells are resistant and thus differ from intact BALB/c mice or non-reconstituted BALB/c.nu/nu mice. In keeping with a high efficacy of limited numbers of T cells following minimal reconstitution, the majority of nude mice implanted subcutaneously with allogeneic, irradiated, neonatal thymus grafts are resistant to L. tropica infection. Genetic features of the nude mouse, such as defect in skin, may contribute to the extraordinary potency of T cell reconstitutive manipulations. Evidence was obtained that BALB/c.nu/nu skin on BALB/c.nu/+ recipients did not support the development of lesions as readily as normal skin sites.

Animals↗
PubMed1980

Antibodies to Leishmania tropica promastigotes during infection in mice of various genotypes.

After intradermal injection of 10(7) promastigotes of a particular isolate of the intramacrophage protozoan parasite, Leishmania tropica, the development of disease (cutaneous lesions) is much more severe in BALB/c than in three other mouse strains, C57BL/6,C3H/He and CBA/H. Using fixed promastigotes and 125I-labelled protein A in a solid-phase radioimmunoassay (RIA), titres of antibody were shown to increase up to about day 50 of infection. However, titres were not markedly different in BALB/c mice compared with the other three resistant strains, although antibody levels were highest in sera from the diseased BALB/c mice at late time points. Using isotype-specific antisera in the RIA, and sera from the day 50 time point, the isotype distribution of anti-promastigote antibodies was not noticeably different in sera from mice of the four genotypes with IgG1 and IgG2a (+/- IgG2b) antibodies predominating. It is concluded that differences in susceptibility to disease in this murine model of cutaneous leishmaniasis do not correlate with any decrease or increase in any particular antibody response to the promastigote.

Animals↗
PubMed1971

Laban Edwin Vine.

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