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Biomedical subjects

J M Dewdney

Publications and source records attributed to J M Dewdney.

36 records · Page 2Linked to original sources
PubMed1982

Development and use of radioimmunoassays for the detection of human IgG antibodies to laboratory animal urinary proteins.

A radioimmunoassay method for the detection of human IgG antibodies to laboratory animal urinary proteins has been developed using partially purified protein fractions prepared from crude urine extracts by gel filtration. The proteins from the four species, mouse, rat, guinea pig and rabbit, were labelled with 125I and used in an antigen binding assay employing a sepharose-linked staphylococcal protein A immunosorbent. Results, expressed as arbitrary units of specific IgG per unit serum, show antibodies to one or more species present in 57% of current animal workers compared with 6% of non-animal workers.

Animals↗
PubMed1978

A clinical environmental study of the aeroallergens of the islands of Bermuda.

The major allergen responsible for allergic respiratory disease in the islands of Bermuda has been shown to be derived from Dermatophagoides pteronyssinus. 73% of the atopic group included in the survey gave weal and erythema reactions to extracts of this mite, whereas only 30% reacted to mixed pollen extracts and 10% to mould extracts. D. pteronyssinus was isolated from all house dust samples and it represented nature, with only a modest seasonal influence, and pollen counts were low throughout the year.

Adolescent↗
PubMed1976

Antigenic properties of polymers formed by beta-lactam antibiotics.

Purified polymers have been isolated from 6-aminopenicillanic acid and from semi-synthetic penicillins and cephalosporins. All the polymers were shown to react with rabbit antibody of penicillins and cephalosporins. All the polymers were shown to react with rabbit antibody of penicilloyl specificity, as demonstrated by passive cutaneous anaphylaxis in guinea pigs, and the reactions were shown to be penicilloyl-specific by hapten inhibition experiments. The cephalosporin-derived polymers in addition reacted with rabbit antibodies raised to the corresponding cephalosporin conjugates of bovine gamma-globulin. Using direct skin tests, passive cutaneous anaphylaxis and an in vitro assay, no evidence was obtained that the polymers induced the formation of specific antibodies in baboons, guinea pigs and rabbits, but in baboons the induction of cell-mediated immunity, demonstrable by delayed skin test reactions, was shown.

Ampicillin↗
PubMed1975

Standardization of Dermatophagoides pteronyssinus extracts and tyrosine adsorbates.

Human skin test, inhibition of RAST and the radioimmunosorbent technique of Ceska have been shown to be useful assays for the standardization of D. pteronyssinus aqueous extracts. The development of these assay systems for the standardization of D. pteronyssinus tyrosine adsorbed formulations is described. Tyrosine solubilisation procedures are detailed together with the influence of these procedures on the three assays. Good correlation has been shown between the human skin test and the radioimmunosorbent technique. The inhibition of RAST appears to be more sensitive than the other techniques to mild conformational changes in the allergen, induced in this work by acid pH. This may limit its value for the standardization of D. pteronyssinus tyrosine adsorbates but it may prove useful in monitoring manufacturing processes.

Adsorption↗
PubMed1970

Reduction in incidence of ampicillin rash by purification of ampicillin.

Purification of ampicillin (Penbritin) with respect to protein impurities has been found significantly to reduce the incidence of rashes in treated patients. This may be related to findings in animals that injections of the isolated protein impurity can induce the formation of circulating IgG antibodies and skin-sensitizing antibodies.

Ampicillin↗
PubMedDate not supplied

Purapen G.

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Journal Article↗