Biomedical subjects

J M Goebel

Publications and source records attributed to J M Goebel.

3 recordsLinked to original sources

PubMed1985

Interferon-induced inhibition of Chlamydia trachomatis: dissociation from antiviral and antiproliferative effects.

The yield of infectious Chlamydia trachomatis was analyzed in human (HeLa) and mouse (McCoy) cell lines treated with the human interferon (IFN) subtypes IFN-alpha A and IFN-alpha D, with their hybrids [IFN-alpha AD (BglII), IFN-alpha AD (PvuII), and IFN-alpha DA (BglII)] constructed in vitro from their expression plasmids, or with IFN-beta 1 or buffy coat IFN. In HeLa cells, a significant inhibition of Chlamydia infectivity was obtained with IFN-alpha D, IFN-alpha DA (BglII), and buffy coat IFN. In McCoy cells, IFN-alpha AD (BglII) and IFN-alpha AD (PvuII) induced a strong degree of inhibition of Chlamydia infectivity. In McCoy cells, there was a correlation among the antichlamydial, antiviral, and antiproliferative activities of the different IFNs tested. In HeLa cells, however, the ability of a particular IFN subtype to inhibit Chlamydia infectivity did not always correlate with its inhibitory effects on encephalomyocarditis virus replication or with its antiproliferative activity.

Cells, Cultured↗

PubMed1984

Ultrastructural analysis of the growth cycle of Chlamydia trachomatis in mouse cells treated with recombinant human alpha-interferons.

The effect of two recombinant human hybrid interferons (IFNs), IFN-alpha AD (BglII) and IFN-alpha DA (BglII), on the growth cycle of Chlamydia trachomatis in a murine (McCoy) cell line was investigated. Ultrastructural analysis indicated that IFN-alpha AD inhibited the growth of chlamydia while IFN-alpha DA-treated cells did not significantly differ from the control monolayers. Treatment of the chlamydia-infected monolayers with IFN-alpha AD resulted in an inhibition in the transformation of elementary bodies to reticulate bodies with a consequent marked decrease in the number of chlamydia inclusions. Furthermore, chlamydial inclusions in the IFN-alpha AD-treated cells contained fewer and more immature chlamydial forms than the control or the IFN-alpha DA-treated cells. Secondary infection occurred in the IFN-alpha DA and in the control monolayer, but no such phenomena was detected in the IFN-alpha AD-treated McCoy cells indicating a loss of infectivity of the chlamydial organisms. From this study it can be concluded that purified recombinant human hybrid IFNs may exert an inhibitory effect on the growth cycle of C. trachomatis in a mouse cell line. This inhibition occurs primarily at the point of transformation from elementary to reticulate body.

Animals↗

PubMed1979

Use-effectiveness and client satisfaction in six centers teaching the Billings Ovulation Method.

Use-effectiveness of the Billings Ovulation Method (OM) is reported for 1139 clients from six centers who were followed for at least 24 months. Method failure rates were 1% when calculated by either Pearl formula or life table analysis. Combined method and user failure rates were 20% when calculated with the Pearl formula, and 16% at 12 months and 23% at 24 months by life table analysis. Pregnancy rates were higher for couples using OM alone, than for couples using OM in combination with additional fertility awareness methods or together with barrier methods. The difference was significant when estimated by the Pearl formula but not when analyzed by the life table. The emotional implications underlying the apparent contradiction between a desire to avoid pregnancy and the deliberate coital use of fertile days are reflected in the differences between method and user failure rates, the high client satisfaction levels reported with "user failure," and 56% continuation rate at 24 months. The will require in-depth exploration.

Abortion, Legal↗