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Biomedical subjects

J M Goodson

Publications and source records attributed to J M Goodson.

18 recordsLinked to original sources
PubMed1992

Antibiotic resistance of the subgingival microbiota following local tetracycline therapy.

The antibiotic resistance of the subgingival microbiota was studied by 3 approaches. First, we assessed the ability of subgingival isolates taken following therapy to grow on media containing tetracycline (TC). Higher percentages of TC-resistant organisms appeared at TC fiber-treated periodontal sites and within the saliva 1 week after treatment as compared with pre-treatment levels. By 1 month, the percentage of TC-resistant organisms had returned to levels comparable to those seen before treatment. In the second approach, subgingival isolates taken following therapy were grown on media without antibiotics, and isolates were selected for Gram-stain and cell morphology determination. This study indicated that subgingival sites became colonized with gram-positive cocci in the same time period that an increase of TC-resistant isolates was observed in the first study. This may account for the transient increase in TC resistance, because many gram-positive cocci are intrinsically resistant to TC. In the third approach, the antibiotic resistance of subgingival gram-negative species was determined. The predominant cultivable microbiota of 9 sites from 3 subjects were isolated immediately before and 6 months after TC fiber treatment. Gram-negative rods were characterized and tested for sensitivity to TC (minimum inhibitory concentration [MIC] 1-128 micrograms/ml), penicillin at 80 micrograms/ml, and erythromycin at 8 micrograms/ml. None of the gram-negative rods were resistant to TC (MIC greater than or equal to 16 micrograms/ml), either before or after treatment. Before treatment 98% of the gram-negative rods were susceptible to TC at 1-2 micrograms/ml and after therapy 88% were susceptible.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1992

Subgingival temperature (I). Relation to baseline clinical parameters.

44 subjects ranging in age from 14-71 years were measured at 6 sites per tooth for gingival redness, plaque accumulation, suppuration, bleeding on probing, pocket depth and attachment level. Subgingival temperatures were measured at the same 6 sites per tooth using a periodontal temperature probe (Periotemp, Abiodent, Danvers, MA). This instrument was also used to measure each subject's sublingual temperature in order to compute the differences between sublingual and subgingival temperature. Relationships were sought between the baseline clinical parameters and the temperature variables in subjects and at sites. The mean and standard deviation of the sublingual temperatures for the 44 subjects was 36.6 +/- 0.4 degrees C (range 35.8-37.6 degrees C). The mean of each subject's mean whole mouth subgingival temperature was 1.9 degrees C lower, 34.8 +/- 0.6 degrees C (range 33.4-36.1 degrees C). The differences of the mean subgingival temperature from sublingual ranged from -0.8 to -3.2 degrees C (average -1.9 +/- 0.5 degrees C). Mean temperature difference for a subject correlated with % of sites with plaque (rs = 0.45), redness (rs = 0.33), bleeding on probing (rs = 0.44), % of sites with attachment level greater than 3 mm (rs = 0.44), mean pocket depth (rs = 0.44) and mean attachment level (rs = 0.39). There were higher mean temperatures at sites exhibiting or not exhibiting plaque (35.0, 34.5 degrees C), redness (34.9, 34.6), bleeding on probing (35.1, 34.7) and suppuration (35.4, 34.8). Sites with pockets less than 4, 4-6 and greater than 6 mm had mean temperatures of 34.6, 35.2, 35.8 degrees C, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)

Adolescent↗
PubMed1992

Subgingival temperature (II). Relation to future periodontal attachment loss.

The purpose of the present investigation was to determine whether subgingival temperature was a risk indicator of periodontal attachment loss (detected in the following 2 months) in a subject or at a site. 29 subjects were measured at 6 sites per tooth for clinical parameters as well as subgingival temperature using a periodontal temperature probe (Periotemp, ABIO-DENT, Danvers, MA). The same instrument was used to measure sublingual temperature in order to compute differences between subgingival and sublingual temperature. Clinical and temperature parameters were measured at 2-month intervals. A total of 49 subject visits which had both baseline temperature and subsequent attachment level change measurements were available for analysis. Attachment level loss greater than 2.5 mm occurred at 1 or more sites at 16 of 49 subject visits. Elevated mean subgingival temperature was related to subsequent attachment loss particularly in individuals who exhibited more than 1 progressing site. The odds ratios of a subject exhibiting new attachment loss at 1 or more sites or at 2 or more sites were 14.5 and 64.0 if the subject's mean subgingival temperature exceeded 35.5 degrees C. Subjects with high mean subgingival temperatures and widespread periodontal destruction appeared to be at greatest risk for new attachment loss. Discriminant analysis using % of sites with suppuration, redness and attachment level greater than 3 mm and mean site temperature correctly "predicted" disease activity with a sensitivity, specificity and overall agreement of 0.75, 0.76 and 0.82 respectively. Of 7243 sites, 43 (0.59%) and 160 (2.2%) showed attachment loss of either 2.5 mm or more or 2 mm or more respectively.(ABSTRACT TRUNCATED AT 250 WORDS)

Adolescent↗
PubMed1992

Subgingival temperature (III). Relation to microbial counts.

The present investigation examined the relationship of selected bacterial species and subgingival temperature. 35 subjects were measured at 6 sites per tooth for clinical parameters and subgingival temperature. Measurements were repeated for 21 subjects at 2 month intervals providing a total of 66 subjects visits. At each visit, subgingival plaque samples were taken from the mesial aspect of each tooth and anaerobically dispersed, diluted and plated on non-selective media. After anaerobic incubation, colonies were lifted to nylon filters and specific species detected using digoxigenin-labeled whole chromosomal DNA probes. Species enumerated were; A. actinomycetemcomitans serotypes a and b, B. forsythus, B. gingivalis, B. intermedius I and II, C. ochracea, F. nucleatum ss. vincentii, P. micros, S. intermedius, S. sanguis I and II, V. parvula and W. recta. Total viable counts and counts of Capnocytophaga sp. were determined directly from the primary isolation plates. A total of 1581 samples were evaluated. Subject visits with higher mean subgingival temperatures had significantly higher mean %s of B. intermedius I and P. micros, and lower mean %s of Capnocytophaga sp. Sites with higher subgingival temperatures had elevated proportions of B. intermedius I and II, A. actinomycetemcomitans serotype a and B. gingivalis more frequently than sites with lower temperatures, while Capnocytophaga sp. were elevated more often at cooler sites. 43 of the subject visits had follow up attachment level measurements at 2 months. The 1026 microbial samples and the subgingival temperature measurements from these visits were related to longitudinal attachment change.(ABSTRACT TRUNCATED AT 250 WORDS)

Adolescent↗
PubMed1992

Conduct of multicenter trials to test agents for treatment of periodontitis.

The conduct of multicenter trials to test agents for treatment of periodontitis is conceptually similar to that of single center trials, but the requirement that centers ultimately be combined into a single result places much more stringent requirements on uniformity and control. Multicenter trials should be considered only if numbers of subjects or the ability to generalize to other treatment settings becomes a driving theme. General guidelines for the conduct of studies of this sort are suggested and problems that occur are discussed. Despite formidable difficulties that may occur, multicenter studies provide a convincing demonstration of an agent's therapeutic efficacy for the treatment of periodontitis.

Clinical Trials as Topic↗
PubMed1992

Diagnosis of periodontitis by physical measurement: interpretation from episodic disease hypothesis.

Physical measurements including the evaluation of probing depth, bleeding on probing, tooth mobility, and inflammation form the basis for most periodontal diagnostics in use today. The interpretation of these observations and the methods available for their measurement, however, have begun to change significantly. The episodic disease activity concept has done much to implement these changes. Observation of episodic attachment loss has been correlated with parallel radiographic changes, alteration in levels of probable pathogens, and changes in inflammatory mediator levels. The failure of pocket depth, suppuration, and bleeding on probing to predict episodic attachment loss has been given plausible explanations and enhanced meanings. Although attachment loss by a continuous process cannot be excluded in some disease conditions, the hypothesis of periodontal disease progression by episodic activity supplements and expands understanding of the disease process. Interest in periodontal diagnostics has accelerated in the last decade. As a parallel development, the technology of small computers has decreased in cost and increased in sophistication. The combination of these factors has created an environment for the development of intelligent diagnostic systems. Four commercially available systems and two systems under development are described. The systems, which measure pocket depth, pocket depth or attachment level, tooth mobility, and pocket temperature, all utilize computer processing of measurements. The result is to provide a simplified and more meaningful presentation of diagnostic information. As intelligent diagnostic systems prove themselves, some of these instruments are likely to become common to dental practice. The promise of more accurate identification of areas of the mouth that are diseased can increase both the efficiency and effectiveness of periodontal therapy.

Humans↗
PubMed1991

Multicenter evaluation of tetracycline fiber therapy. III. Microbiological response.

In a multicenter study of the effects of tetracycline (TC) fiber therapy, subgingival plaque samples were tested for 6 probable periodontal pathogens by DNA probe analysis. Levels of Actinobacillus actinomycetemcomitans, Eikenella corrodens, Fusobacterium nucleatum, Porphyromonas (Bacteroides) gingivalis, Prevotella intermedia (Bacteroides intermedius), and Wolinella recta were quantitatively determined in samples taken at baseline, and immediately after TC fiber removal, control fiber removal, and scaling and root planing. At untreated sites, samples were taken at baseline and 10 d later. Specificity of the DNA probe method was evaluated by testing the hybridization to 83 reference cultures. Interaction of the F. nucleatum probe with Fusobacterium periodonticum, and of the W. recta probe with Wolinella curva were the only cross-hybridizations noted. Species were detected at an average sensitivity of 2.9 x 10(4) organisms per sample. Approximately 70% of sites were initially infected with P. gingivalis and F. nucleatum; 50% with P. intermedia and E. corrodens; infections with W. recta and A. actinomycetemcomitans were less common (36% and 11% respectively). The average numbers of organisms found in the plaque samples were highest for F. nucleatum, P. gingivalis, and P. intermedia (ca. 10(6)). E. corrodens, W. recta, and A. actinomycetemcomitans occurred at 10-fold lower levels. Bacterial numbers and proportions of species in subgingival sites from the five centers did not differ appreciably. Both TC fiber therapy and scaling decreased the number of sites infected with all the monitored species. The bacterial composition at untreated sites and at sites where control fibers were placed was not significantly altered. The percentage reduction of the number of sites with detectable infection varied with each species: from 86% with W. recta to approximately 40% with P. gingivalis. Significant reduction of pocket depth and bleeding occurred at TC fiber-treated sites infected with each of the species. Significant attachment level gain occurred only at sites initially infected with P. gingivalis and treated with TC fibers.

Actinobacillus↗
PubMed1991

Tetracycline fiber therapy monitored by DNA probe and cultural methods.

Oligonucleotide DNA probe and selective cultural methods were compared in their ability to monitor 6 putative periodontal pathogens in a study evaluating local tetracycline fiber therapy. Subgingival plaque was sampled from 4 sites in each of 20 subjects. Samples were taken before and after therapy from sites assigned to the following test groups: tetracycline (TC) fiber, scaling and root planing, control fiber, and untreated. Each sample was analyzed by both DNA probe and cultural methods. Total anaerobic cultivable counts, Porphyromonas (Bacteroides) gingivalis and Prevotella intermedia (Bacteroides intermedius) were enumerated on nonselective blood agar. Actinobacillus actinomycetemcomitans, Eikenella corrodens, Fusobacterium nucleatum and Wolinella recta were isolated on selective media. TC fiber therapy and scaling reduced total cultivable counts from an initial value of 1 x 10(7) to approximately 2 x 10(5) following therapy. Total counts at untreated sites and at sites with control fibers did not change from baseline. A. actinomycetemcomitans and E. corrodens were detected more frequently by the cultural method; the other monitored species were detected more frequently by DNA probes than by the cultural methods. Agreements between methods were: 77.2% for A. actinomycetemcomitans; 72.2% for P. intermedia; 75.6% for E. corrodens; 39.4% for F. nucleatum; 35.6% for P. gingivalis; and 68.9% for W. recta. Limitations of the selective cultural methods used probably contributed to the discrepancies for P. gingivalis and F. nucleatum. DNA probe and cultural methods indicated comparable levels of suppression of the monitored species following TC fiber therapy and scaling. The microbiota of control fiber and untreated sites did not appear to be significantly altered by either method.

Actinobacillus↗
PubMed1991

Multicenter evaluation of tetracycline fiber therapy: I. Experimental design, methods, and baseline data.

The study design and baseline characteristics of a multicenter trial to test the effectiveness and safety of locally delivered tetracycline for treatment of adult periodontitis are described. Local delivery was provided by 0.5 mm diameter ethylene vinyl acetate copolymer fibers loaded 25% with tetracycline hydrochloride which were placed into periodontal pockets and maintained by an adhesive for 10 (+/- 2) days. A total of 113 subjects (56 male and 57 female; mean age 49.3 yr) at five centers participated in the study. Subjects were selected who had 4 nonadjacent teeth with 6-10 mm pockets that bled on probing. The selected sites in each subject were randomly assigned to 4 test groups: tetracycline fiber, control fiber, scaling with root planing, or untreated. A balanced experimental design was thereby established in which each subject contributed equally by providing 4 clinically comparable sites for evaluation. To provide a more specific model for testing periodontitis therapy, gingivitis was treated prior to the initiation of the study by prophylaxis with supragingival calculus removal and home care instruction. Clinical response variables measured were pocket depth reduction, attachment level gain and bleeding on controlled-force probing measured at baseline, 30 d, and 60 d. Levels of 6 bacterial species selected as probable periodontal pathogens were measured by DNA probe analysis of plaque samples. The design of this study provided several unique analytical opportunities. Controls included a comparison with conventional treatment, analysis of vehicle effects, and effects at untreated sites. Comparison of the test group with controls permitted evaluation of the principal variables that could effect interpretation of results.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1991

Multicenter evaluation of tetracycline fiber therapy: II. Clinical response.

The safety and efficacy of periodontal disease treatment by intrapocket placement of tetracycline (TC) fibers was investigated in a 60-day multicenter study conducted by selecting 4 sites in each subject with 6-10 mm pockets that bled on probing. Sites were randomly assigned to 1 of 4 test groups: TC fiber therapy, scaling, control fiber (fibers without drug), or untreated. TC fibers and control fibers were placed to fill the pocket and were maintained with a cyanoacrylate adhesive for 10(+/- 2) d. Scaling was performed for a minimum of 5 min under local anesthesia. Following initial tooth cleaning procedures, pocket depth, attachment level and bleeding on controlled-force probing were measured at baseline and at 30 d, and 60 d following therapy. Analysis of data from 107 subjects who had complete clinical data sets indicated that TC fiber therapy significantly decreased pocket depth, increased attachment level, and decreased bleeding on controlled-force probing to a greater extent than observed in all other test groups including scaling. These effects were greater than, and in addition to, effects that occurred due to prophylaxis and improved home care. No serious adverse side-effects attributed to TC fiber therapy were observed. No TC fiber-treated sites abscessed and superinfection was not noted. A transient redness at fiber removal was seen at 21% of the sites. Although fibers were placed without anesthesia, mild pain on initial placement was infrequent (19%) and abated rapidly. The results indicate that TC fiber placement provides a safe and effective means for treatment of periodontal infections.

Adult↗
PubMed1979

Periodontal therapy by local delivery of tetracycline.

The present investigation assessed the feasibility of treating periodontal disease by controlled delivery of antibacterial agents from within periodontal pockets. Tetracycline-filled hollow fibers placed in the gingival sulcus were shown to have a dramatic effect both on the periodontal microflora and clinical manifestations of disease. Furthermore, it was found that drug-filled cellulose acetate hollow fibers are biologically compatible with the environment and can be manipulated by dental personnel to provide drug therapy with less than 1/1000 the amount of tetracycline that would have been used for systemic therapy. Of theoretical importance is the observation that virtual elimination of spirochetes from the gingival sulcus is possible by a single placement of tetracycline-filled hollow fibers, and spirochetes, once eliminated from a site, do not rapidly recolonize despite the persistence of viable organisms elsewhere in the mouth.

Delayed-Action Preparations↗
PubMed1977

Evaluation of oral submucosal blood flow at dental injection sites by radioactive Xenon clearance in beagle dogs.

Oral submucosal blood flow was measured at dental injection sites in anesthetized dogs and compared with subcutaneous blood flow. Blood flow was calculated from the measured half-time for clearance of radioactive xenon dissolved in saline. By this method, oral submucosal blood flow was much greater than subcutaneous blood flow and was comparable in magnitude to values reported for cerebral blood flow. Injection of a solution containing 1:100,000 epinephrine at the mandibular canal produce a delayed clearance of isotope which was three times as long as the clearance time for solutions without epinephrine.

Anesthesia, Dental↗
PubMed1977

Initiation of bone resorption by the classical and alternative C pathways and its mediation by prostaglandins.

Activation of C by immunoglobulins reactive with cell surface antigens stimulated synthesis of prostaglandin E and resultant release of 45Ca from organ cultures of fetal rat bones labeled in utero. Absorption of the C source (rabbit serum) with rat spleen cells or dilution of C abolished this activity which was, however, restored by addition of immunoglobulin preparations from sera of rabbits immunized with either rat erythrocytes or sonicated fetal rat bones. The active reconstitution factors were present in the 50% (NH4)2SO4 cut of the antisera and were eluted from Sephadex G-200 in both the 19S and 7S fractions and from DEAE cellulose in the IgG-containing fraction. The C-dependent resorption of bone by the F(ab')2 fragments of the IgG antibody implicated a role for the alternative C pathway in this event. Complete inhibition of this biologic effect by indomethacin and detection of enhanced levels of prostaglandin E in the media of cultures containing antibody and C demonstrated a role for prostaglandins as mediators in the destruction of bone initiated by immune activation of C.

Animals↗
PubMed1975

Spermatic granulomas of epididymis.

This report is of a patient with spontaneous spermatic granuloma of the epididymis, an uncommon condition to occur without antecedent surgery, trauma, or clinical infection.

Epididymis↗
PubMed1975

Stimulation of bone resorption by various prostaglandins in organ culture.

The ability of E, F, A and B prostaglandins to stimulate bone resorption was demonstrated in organ culture. All of the compounds tested were able to increase the release of previously incorporated 45Ca from fetal rat bone by 60 to 135 per cent at maximally effective doses, but prostaglandins of the E series were 10- to 100- fold more potent than F, A or B prostaglandins. Compounds with two double bonds in the side chain were usually more potent than those with one double bond. PGE2 stimulation of bone resorption increased linearly with the logarithm of the medium concentration over the range of 10(-9)M to 10(-5)M, then decreased at higher concentrations. PGE2 stimulated bone resorption more slowly than did parathyroid hormone but caused complete resorption after six days in the culture system. Equilibrium dialysis studies showed no significant binding of F, and 16-34% binding of E and A prostaglandins to bovine serum albumin, which was present in the medium at 1 mg/ml. These differences in albumin binding could not account for differences in potency.

Animals↗
PubMed1975

Analysis of human mandibular movement.

The parametric electrical outputs of the six-transducer mandibulograph were used to analyze three-dimensional pathways, displacement profiles, and velocity profiles of selected mandibular areas during jaw movement. This was accomplished by means of digital computer implementation of derived mathematical equations of motion. Five jaw movements were studied: chewing, open-close, right lateral, left lateral and protrusive. The results demonstrate the analytical and diagnostic value of this method and provice new clarification and insight into the nature of mandibular movements. The following observations summarize the principal findings derived from the analysis of jaw movement patterns of a normal patient. The chewing jaw movement exhibited a smoothly coordinated sequence of events. Lateral jaw motion appeared to be prominent during the masticatory phase and throughout the movement cycle. Mastication was abruptly terminated by a vertical desscent of the mandible through the freeway space, until the condyles began their forward movement producing a rotation in the sagittal plane. T occlusal area continued its downward descent along a nearly vertical pathway producing a compressed figure eight pattern in the frontal plane. The open-close jaw movement exhibited minor periods of hesitancy. Jaw movement was initiated with a forward and downward movement of the condyle occurring at the same time as a downward and backward movement of the occlusal area. This motion produced a rotation in the sagittal plane about a moving axis which was not coincident with the intercondylar axis. The general form of the command open-close jaw movement was that of noncoincident curved pathways in the sagittal plane with little lateral displacement. Closing jaw movement occurred with the condyle in a protruded position...

Adult↗
PubMedDate not supplied

Computer-facilitated nutritional analysis.

A seven-day dietary analysis of 59 students in the dental health profession indicated that their nutrient consumption was generally adequate. A dental health examination indicated excellent oral hygiene. Nevertheless, many students were deficient in specific nutrient categories. Results suggested that computerized nutritional analysis techniques have general applicability in dental practice and epidemiological testing.

Ascorbic Acid↗