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Biomedical subjects

J M Kennedy

Publications and source records attributed to J M Kennedy.

At least 37 records · Page 2Linked to original sources

Blind children recognizing tactile pictures respond like sighted children given guidance in exploration.

Theory of tactile pictures argues that untrained blind subjects can recognize raised, outline pictures. It contends the blind person's knowledge of the shapes of common objects is like that of the sighted, and the blind person's pictorial abilities use the same principles as the sighted person's. To test this theory, blind children (aged 8-13) and blindfolded age-matched sighted children were asked to identify raised-line drawings of common objects. Their performances were correlated. In addition, the blind children identified more than sighted children exploring the pictures actively, but the same number of pictures as sighted children who were given passive, guided exploration. We argue blind and sighted children use the same principles to identify the pictures, but the blind have superior exploration skills. The differences in the effects of exploration skills on recognition scores are minimized when the sighted children are given guidance, since the sighted children then have efficient contact with the displays, and the performance of the sighted and the blind is then governed by the same principles, without one group benefitting from advantages in exploration skills.

Adolescent↗

Developmentally regulated expression of cytochrome-c oxidase isoforms in regenerating rat skeletal muscle.

The developmental expression of tissue-specific isoforms of cytochrome-c oxidase (COX) subunit VIII [heart (COX VIII-H) and liver (COX VIII-L)] and the influence of innervation were examined in regenerating fast [extensor digitorum longus (EDL)] and slow (soleus) muscles. In adult muscles, COX VIII-H was the predominant isoform. The COX VIII-L mRNA was expressed 3 days after induction of regeneration, and it progressively decreased after 7, 10, 14, and 30 days of regeneration in both muscles. In contrast, the expression of COX VIII-H mRNA accumulated as myogenesis proceeded to the myotube stage between 7 and 10 days of regeneration and progressively increased to near control levels by 30 days. The influence of innervation on the expression of COX VIII and alpha-actin isoforms was examined in control, innervated, and denervated regenerating muscles at 3 and 10 days. The relative expression of COX VIII-L mRNA in denervated regenerating EDL muscles was significantly greater, while that of COX VIII-H was significantly less than in innervated regenerating EDL muscles after 10 days of regeneration. Similarly, cardiac alpha-actin mRNA levels were elevated in denervated regenerating EDL muscles after 10 days of regeneration. In conclusion, motor innervation influences the transition from the COX VIII-L to COX VIII-H isoform during myogenesis in regenerating muscles.

Actins↗

Effects of surgery on the pharmacokinetic parameters of drugs.

As with other forms of stress, surgery is a time of fluctuating haemodynamics, physiological shifts, intense metabolic changes, and protein catabolism and anabolism. At times, these changes can occur within hours. There is a reasonable body of literature concerning the adverse effects of surgery on gastric emptying, but not on the effects of surgery on the distribution, metabolism and excretion of drugs. Gastric emptying is diminished or absent following major procedures, which prevent the delivery of orally administered drugs to their major site of absorption, the small bowel. Changes in the paracellular absorption of drugs may occur postoperatively, although transcellular absorption appears to be unimpaired. Distribution is affected by changes in blood volume, alterations in circulation, increases in the extracellular fluid and changes in the circulating plasma protein levels, such as albumin and alpha 1-acid glycoprotein. Little is known about alterations in drug metabolism following surgery. However, it has been shown that systemic hypoxia alters the function of some of the cytochrome P450 (CYP) system and increased levels of cytokines have an effect on the metabolism of at least 1 drug. In addition, the renal elimination of drugs is affected in patients postoperatively, although the effects of biliary clearance in this period are difficult to determine. Despite the lack of research into pharmacokinetics during the postoperative period, and given the immense and often sudden changes observed in patients post-surgery, it is reasonable to recommend vigilance with respect to drug therapy during this period.

Absorption↗

Influence of thyroid hormone on the tissue-specific expression of cytochrome c oxidase isoforms during cardiac development.

In mammals, cytochrome c oxidase (COX) is composed of 13 different protein subunits. In the rat, two nuclear-encoded subunits, COX VIa and VIII, exist as tissue-specific isoforms: heart and liver. Using Northern-blot analysis, the levels of transcripts for the heart and liver isoforms of VIa and VIII were examined in developing rat hearts. The liver isoform was found to be the predominant form of subunit VIa and the exclusive form of VIII in the 18-day fetal hearts. The mRNA levels of the heart isoform of both subunits increased dramatically to reach adult levels by 14 days. Although the levels of the VIa- and VIII-liver isoform mRNAs remained stable throughout early development, their levels decreased by 40 and 36% respectively between the 18-day fetal stage and 18-day neonatal stage. Therefore the up-regulation of the heart isoforms and down-regulation of the liver isoforms appear to be regulated in a co-ordinated manner during development. To determine if thyroid hormone influences the expression of these developmentally regulated isoforms, the RNA was also extracted from the hearts of 2-week-old hypothyroid rats. The results showed that the levels of VIII-heart and VIa-liver COX mRNAs were approx. 40% lower in the hypothyroid hearts, while VIII-liver and VIa-heart COX isoform expression remained unchanged. These data demonstrate that the isoforms of COX subunits VIa and VIII are not co-ordinately regulated by changes in thyroid hormone levels. Therefore we conclude that, although thyroid hormone influences the expression of isoforms, it appears to do so via a different mechanism from that which regulates the developmental transition.

Animals↗

Long-term compressive property durability of carbon fibre-reinforced polyetheretherketone composite in physiological saline.

In total hip arthroplasty, concerns such as corrosion and stress shielding associated with stiff metallic femoral components have led to the development of low stiffness advanced fibre-reinforced polymer (FRP) composite femoral components. Carbon fibre-reinforced polyetheretherketone (CF/PEEK) composite material is now one of the primary material systems being considered for composite hip stem development. As a hip stem, a composite material must be able to support a complex state of stress in the in vivo environment without failure. Considering the loading conditions of a hip stem (superimposed compression and bending), and the fact that FRP composites typically possess lower compressive than tensile strength, the compressive behaviour of FRP composites becomes very important for femoral component design. This paper presents an investigation of the long-term durability of 0 degree and 90 degrees compressive strengths of CF/PEEK composite following physiological saline saturation. 0 degree and 90 degrees compressive moduli and Poisson ratio (v12) properties are also reported. Samples were tested following conditioning in physiological saline at 37, 65 and 95 degrees C for time periods from 0 to 5000 h. Dry samples were tested as controls. Results show no significant loss in compressive property values of the saline-saturated or the dry control samples as a function of conditioning time or temperature.

Benzophenones↗

Skeletal muscle mitochondria from AZT-treated rats have a diminished response to chronic electrical stimulation.

Inhibition of DNA polymerase gamma-function mediated by 3'-azido-3'-deoxythymidine (AZT) has been proposed to cause a myopathy by reducing mitochondrial DNA (mtDNA) replication. Repeated bouts of exercise stimulate an increase in mtDNA replication, mitochondrial content, and mitochondrial volume fraction. Therefore, adaptation of rat skeletal muscle [tibialis anterior (TA)] mitochondria exposed to AZT (1 mg/ml for 35 days) and then to electrical stimulation for 8 h/day (7, 14, 21 days) with continued AZT treatment was examined. Fourteen and 21 days of stimulation increased TA cytochrome oxidase (CO) activity, mtDNA, and CO subunit III and VIc mRNA levels in both groups. The TA CO activity and CO III mRNA increases after 14 and 21 days of stimulation were diminished in AZT-treated rats. TA glyceraldehyde-3-phosphate dehydrogenase was reduced in normal rats after chronic stimulation but was unchanged in AZT-treated rats. Chronic stimulation increased the mitochondrial volume fraction by 80 and 40% in normal and AZT-treated rats, respectively. These results indicate diminution, but not complete inhibition, of mitochondrial adaptation by AZT-treated skeletal muscle in response to stimulation.

Animals↗

Specification and initial evaluation of a multiple application teleradiology system.

The digitization and transmission of medical images is becoming increasingly more important and available. The selection of appropriate clinical applications for teleradiology systems is an important factor in determining the success of such ventures. In this paper, the selection of such applications and the evaluation of the teleradiology system is described. The system (LIAISON, CAPTEC Ltd, Malahide, Ireland) is PC based and is capable of digitization and transmission of three categories of medical images: (1) plain film radiographs; (2) CT film data and (3) video sequences such as ultrasound scans. A comprehensive technical evaluation was carried out on the acquisition and display station in which various parameters such as spatial resolution, signal-to-noise ratio (SNR), and distortion were investigated. Results showed that the system performed well within specifications. An exploratory clinical evaluation was performed using a case mix of subtle fractures, chests and dislocations, and CT scans. Here the system performed well with a diagnostic accuracy of 95% for the digital image compared with the analogue image.

Computer Communication Networks↗

Differential expression of TnI and TnT isoforms in rabbit heart during the perinatal period and during cardiovascular stress.

We have investigated developmental transitions of TnI and TnT isoforms in fetal, neonatal, and adult rabbit hearts by western blot analysis. Our results provide the first evidence for the existence of two developmentally regulated isoforms of TnI in rabbit heart. These isoforms comigrate with adult rabbit cardiac TnI (cTnI) and slow skeletal TnI (ssTnI). At 23 days of gestation, ssTnI was the predominant TnI isoform. At 29 days of gestation, there was a significant increase in the relative amount of cTnI, that continued with maturation. The TnI isoform transition was significantly faster in right than left ventricles at gestation 30 and 32 days. Four TnT isoforms were detected in fetal rabbit ventricles. The relative amount of TnT isoforms did not change from 23 to 29 days of gestation. However, the relative amount of the adult TnT isoform increased significantly around the time of birth with the increase being significantly more prominent in left than in right ventricles. Maternal injection of phenylephrine (PHE), an alpha-1 adrenergic agonist, increased fetal cardiac force and arterial blood pressure, facilitated TnT, but not TnI, isoform transition in fetal heart. Our results indicate that the developmental transition of rabbit cardiac TnI and TnT isoforms is not coordinated and might be regulated by different mechanisms. Our results also provide evidence that the TnT isoform population is influenced by adrenergic stimulation and stress on the cardiovascular system during development.

Aging↗

Production and interpretation of pictures of houses by blind people.

Subjects were exposed to a three-dimensional model of a house and were asked to draw it using a raised-line drawing kit. Independent groups of ten each of sighted controls, early-blind, and late-blind subjects were told to identify the vantage point of tangible pictures of the model, including side views, 'bird's-eye' views from above, and views involving linear perspective. The ease or difficulty of picture interpretation depended upon the nature of the tangible drawing, with much better performance being recorded for side views. Performance was poor for foreshortened 3/4 views. Early-blind subjects were particularly unlikely to recognize views from above. In a control experiment with blindfolded sighted subjects the influence of prior information was examined: some subjects were told that the drawings could consist of side view or bird's-eye, top view, or 3/4 view drawings. This experiment showed that performance can be greatly improved through prior information about the nature of the tangible pictures.

Adolescent↗

Foreshortening in cube drawings by children and adults.

The lengths of lines and the sizes of angles were measured in freehand drawings of cubes produced by 190 children and 158 adults. The lengths of oblique lines depicting receding cube edges were foreshortened relative to horizontal lines showing nonreceding edges. In the drawings from children aged 9 and 10 years the obliques were foreshortened by about 40%, compared with 30% in adults' drawings. The amount of foreshortening was not correlated with the angle at which the obliques were drawn. Line lengths were also foreshortened in "transparent" drawings, which are often said to show "what is known" about cube structure. One explanation of the results is that line lengths are foreshortened by children and adults to create the visual impression of equal-length cube edges, even in transparent drawings. This foreshortening fits with how the receding edges of a square face of a cube project to a vantage point. Children and adults who use foreshortening are not depicting the structure of cubes by matching features such as equal-length edges with similar features in the physical lines on the page, as has been suggested in some recent approaches to drawing development.

Adolescent↗

Misalignment effects in 3-D versions of Poggendorff displays.

Strong misalignment effects are found in three-dimensional (3-D) versions of Poggendorff displays viewed binocularly. The components of the standard 2-D Poggendorff figure--the parallels and the oblique segments--were presented in 3-D depth as a flat rectangular object with occluding edges and an oblique line situated behind the object. Three experiments investigated the misalignment effects under three different observation instructions: Subjects were told to look at the oblique (Experiment 1), at the rectangle (Experiment 2), or at the background (Experiment 3). Experiments 1 and 2 examined the effects on judgments of alignment of varying the distance in depth that separates the oblique from the rectangle. Experiment 3 examined the effects of varying the distance between the fixated background and the 3-D Poggendorff figure. Both standard and reversed misalignment effects were obtained. When the viewing condition produces crossed disparity for the oblique, perceived misalignment occurs in the usual Poggendorff direction, but it is reversed with uncrossed disparity. Moreover, the amount of misalignment is related to the amount of disparity, and it can be much stronger than is usual in the 2-D versions of the Poggendorff. The misalignment effects can be explained by binocular integration to produce a single cyclopean image.

Adolescent↗

Tricompartmental knee replacement. A comparison of orthopaedic surgeons' self reported performance rates with surgical indications, contraindications, and expected outcomes. Knee Replacement Patient Outcomes Research Team.

The chance of a person with osteoarthritis of the knee receiving a knee replacement is highly variable. To understand better the reasons for this variation, all practicing orthopaedists in Indiana were surveyed about their management of severe knee osteoarthritis and their perception of tricompartmental knee replacement as a therapeutic option. Their perceptions of indications and outcomes of knee replacement were compared with the self reported annual number of patients for whom they performed (or referred to other surgeons for) tricompartmental knee replacements. A completed survey was returned by 220 (79%) of the 280 orthopaedists surveyed; analyses were limited to the 188 respondents who had cared for at least one patient with osteoarthritis of the knee in the prior 2 weeks (mean = 13). These surgeons reported performing (or referring patients for) a mean of 31 knee replacements in the prior year (SD 45, median 21, range 0-480 knee replacements). There was strong agreement (> 95%) among respondents for seven (21%) of 33 surgical indications and contraindications, and more general agreement (> 60%) for 21 (64%). In the five factors (15%) for which there was disagreement, there was no consistent relationship between opinions and self reported knee replacement performance rate. Surgeons reporting more knee replacements had significantly higher estimates of pain relief and functional improvement following surgery, and lower estimates of prosthesis infection and failure rates. When all responses were considered together, four decision factors correlated independently with the performance of more knee replacements, but these four factors explained only 24% of the variation in self reported knee replacement performance.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗

Ventricular mitochondrial gene expression during development and following embryonic ethanol exposure.

The effects of chronic embryonic ethanol exposure were evaluated in chick ventricular muscle. Ethanol treatments were administered on embryonic days 11, 13, 15, and 17 and chicks were sacrificed at various time points following treatments. Fluctuations in embryonic blood ethanol levels were examined following treatments. Developmental increases in the activities of mitochondrial enzymes, cytochrome oxidase (CO) and citrate synthase (CS), were observed. Ethanol exposure resulted in a depression in CO activity, but not CS activity. Since, a maximal depression in CO activity was seen with ethanol treatments of 75 mg/100 g, this dosing paradigm was adopted for subsequent experiments. A tissue-specific effect of ethanol was demonstrated as CO activity was unchanged in atrial, liver, pectoralis, and brain tissues. The role of mitochondrial DNA replication and transcription during the developmental up-regulation and ethanol-induced down-regulation of CO activity was evaluated using a cDNA probe for cytochrome oxidase subunit III (COIII). The relative levels of COIII mRNA and mitochondrial DNA (cpm/mg protein) decreased by 3-fold and 4-fold, respectively, across the developmental time course, while CO activity increased by 3.5-fold. Therefore, increases in mitochondrial DNA and mitochondrial mRNA transcripts are unlikely to be responsible for the developmentally-regulated increases in CO activity. Similarly, embryonic ethanol exposure failed to elicit alterations in COIII mRNA levels, indicating that the ethanol-induced depression in CO activity was not transcriptionally regulated. However, ventricular mitochondrial DNA concentrations were elevated in ethanol-treated embryos, indicating that ethanol-exposure either directly or indirectly induces mitochondrial DNA replication.

Animals↗

Passive sound localization of prey by the pallid bat (Antrozous p. pallidus).

The pallid bat (Antrozous p. pallidus) uses passive sound localization to capture terrestrial prey. This study of captive pallid bats examined the roles of echolocation and passive sound localization in prey capture, and focused on their spectral requirements for accurate passive sound localization. Crickets were used as prey throughout these studies. All tests were conducted in dim, red light in an effort to preclude the use of vision. Hunting performance did not differ significantly in red light and total darkness, nor did it differ when visual contrast between the terrestrial prey and the substrate was varied, demonstrating that the bats did not use vision to locate prey. Our bats apparently used echolocation for general orientation, but not to locate prey. They did not increase their pulse emission rate prior to prey capture, suggesting that they were not actively scanning prey. Instead, they required prey-generated sounds for localization. The bats attended to the sound of walking crickets for localization, and also attacked small, inanimate objects dragged across the floor. Stationary and/or anesthetized crickets were ignored, as were crickets walking on substrates that greatly attenuated walking sounds. Cricket communication sounds were not used in prey localization; the bats never captured stationary, calling crickets. The accuracy of their passive sound localization was tested with an open-loop passive sound localization task that required them to land upon an anesthetized cricket tossed on the floor. The impact of a cricket produced a single 10-20 ms duration sound, yet with this information, the bats were able to land within 7.6 cm of the cricket from a maximum distance of 4.9 m. This performance suggests a sound localization accuracy of approximately +/- 1 degree in the horizontal and vertical dimensions of auditory space. The lower frequency limit for accurate sound localization was between 3-8 kHz. A physiological survey of frequency representation in the pallid bat inferior colliculus suggests that this lower frequency limit is around 5 kHz.

Animals↗

Foreshortening and the perception of parallel projections.

Does picture perception follow polar projective geometry? Parallel projection drawings, which are not produced by using rules of polar projection, are widely regarded as visually acceptable representations of three-dimensional (3-D) objects in free viewing. One explanation is that they are perceived by means of a system in which there is no foreshortening. If so, edges of a 3-D block in 1:1 proportions should be denoted by lines in 1:1 proportions on the picture surface. However, three experiments suggest that the perception of parallel projections of a block involves foreshortening. In Experiment 1, 90 subjects were shown a set of parallel projections of a cube, in which each drawing depicted three sides of the cube, drawn as a square with obliques--a frontal square with receding edges shown by parallel obliques of various lengths. The subjects preferred a drawing with a receding side length that was considerably foreshortened in relation to the front side. In Experiments 2 and 3, subjects viewed drawings of three blocks that differed in the ratios of the lengths of their receding edges to their frontal edges (1:1, 1:2, and 1:0.65). In Experiment 2, the subjects were shown square-with-obliques drawings of the three blocks with receding edges shown by parallel obliques of various lengths. Again, the subjects preferred drawings with a receding side that was foreshortened. In Experiment 3, the drawings showed two sides of a block. The receding dimension was drawn with parallel or converging lines. The preferred foreshortening was not a fixed ratio of the dimensions of the 3-D blocks. We suggest that square-with-obliques parallel projections showing cubes are taken by vision to be approximations to projections using foreshortening. We suggest also that as the line showing the receding edge elongates, foreshortening becomes less of a factor.

Adolescent↗