SELECTIVE SYNTHESIS OF MESSENGER RNA IN A FISSION YEAST DURING A STEP-DOWN, AND ITS RELATION TO THE CELL CYCLE. BULK EXPERIMENTS.
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Biomedical subjects
Publications and source records attributed to J M MITCHISON.
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A method for isolating the mitotic apparatus from dividing sea urchin eggs without the use of ethyl alcohol or of detergents is described. In the present method, the eggs are dispersed directly in a medium containing 1 M (to 1.15 M) sucrose, 0.15 M dithiodiglycol, and 0.001 M Versene at pH 6, releasing the visibly intact mitotic apparatus. The method is designed for studies of enzyme activities, lipid components, and the variables affecting the stability of the apparatus.
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