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J M Pinon

Publications and source records attributed to J M Pinon.

14 recordsLinked to original sources
PubMed1979

Immunological study of hydatidosis. I. Evaluation of immunoelectrodiffusion tests and enzyme-linked immunoelectrodiffusion assay (ELIEDA) in human hydatidosis.

The comparative sensitivity, specificity and rapidity of immunoelectrodiffusion (IED) on cellulose acetate membranes and of immunoelectrophoresis (IEP) on agarose gel, were evaluated in the diagnosis of hydatidosis. Pooled crude fluid from several cattle hydatis cysts was used as antigen in tests on 1,750 non-hydatis and 400 hydatis sera obtained from patients with hepatic, pulmonary, splenic, peritoneal and cerebral hydatidosis before and after surgery. Coupled to a specific human reference serum for arc 5, IED shows a specificity comparable to that of IEP but its sensitivity is slightly higher and the amount of antigen needed is very small. The appearance of a typical "gloved finger" pattern in sera from patients with ruptured cysts emphasizes the interest of quick results (3 hours) obtained by this method. In order to increase the sensitivity of IED and to define the class of immunoglobulins involved in the antigen-antibody reaction, we have coupled this method to an enzymatic technique. The immune complexes precipitated by IED were treated with peroxidase-labelled antibodies specific to each class of human immunoglobulins. The specificity of this enzyme-linked immunoelectrodiffusion assay (ELIEDA) permits one to follow the immunologic evolution of hydatidosis and to identify IgM in ruptured hepatic cysts and IgA in pulmonary cysts.

Antigen-Antibody Reactions↗
PubMed1978

[Precipitating immunoenzyme revealed tests on cellulose acetate. Use of ELIEDA (enzyme-linked-immuno-electro-diffusion assay) and ELIDEPA (enzyme-linked-immuno-double-electrophoresis-assay) in parasitology].

Precipitating tests carried out on cellulose acetate membrane were revealed by treating the immune complexes with enzyme linked anti-immunoglobulin antibodies. We insist on the importance of ELIEDA (enzyme-linked-immuno-electro-diffusion-assay) and ELIDEPA (enzyme-linked-immuno-double-electro-phoresis-assay) for the study of antibody classes involved in the immune response.

Antibodies, Anti-Idiotypic↗
PubMed1978

The use of ELIEDA (enzyme-linked-immuno-electro-diffusion-assay)) in the study of humoral antibodies in human schistosomiasis.

In order to improve the sensitivity of immuno-electrodiffusion (IED) we have developed a technique of treating the precipitated immune complexes with enzyme-labelled antibodies. This technique we term ELIEDA (Enzyme-Linked-Immuno-Electro-Diffusion-Assay). The enzyme-labelled antibodies can be made monospecific for each class of immunoglobulins found in patients with schistosomiasis and which precipitate with schistosome antigens. We believe that this sensitive technique will prove valuable in studying the sequential development of antibodies in human and experimental schistosomiasis.

Antibodies↗
PubMed1977

[Exploration of humoral immunity in aspergillosis: value of enzyme linked immuno-electro-diffusion assay].

In order to increase the sensitivity of the method of immunoelectrodiffusion (I.E.D.) and to isolate the class of immunoglobulins implicated or involved in the Aspergillose antigen-antibody reaction, we supplemented the I.E.D. by combining or incorporating it with an enzymatic technique. By this method the immune complexes precipitated are taken up by antibodies conjugated to one enzyme and specific to the immunoglobulins of each class. This constitutes the technique of E.L.I.E.D.A. (enzyme-linked-immuno-electro-diffusion assay). The sensitivity and the specificity of the proposed method would allow us to follow the qualitative evolution of the aspergillar antibodies and would lend support to further arguments on the evolutionary character of the inflammatory response.

Aspergillosis↗
PubMed1977

ELIEDA (enzyme-linked-immuno-electro-diffusion-assay): application of a combined immunoelectrodiffusion and immunoenzyme method to the study of immune response in parasitic infections.

The sensitivity of the technique of immunoelectrodiffusion (IED) has been enhanced by combining it with an enzyme method; this also enabled the class of immunoglobulins involved in the reactions to be determined by treating the precipitated immune complexes with enzyme-labelled class-specific antisera. The method has been employed in studies of the immunology of parasitic infections and these confirmed its sensitivity and specificity. ELIEDA provides a valuable tool for analysing responses to complex immunogens.

Animals↗
PubMed1977

[Humoral study of human parasitic diseases. Interest of ELIEDA. (Enzyme--linked--immuno--electro--diffusion--assay) (author's transl)].

In order to sensitize the reaction of immuno-electro-diffusion (IED) and to define the class of immuno-globulins implied in the antigen-antibody reaction, we have completed the technique of IED by coupling it to an enzymatic technique. In such a way the immune complexes are treated by enzyme labeled antibodies specific to each class of immunoglobulin. This association realizes the technique of ELIEDA (enzyme-linked-immuno-electro-diffusion-assay). The sensitivity and the specificity of this method allow the follow up of the qualitative evolution of antibodies during natural or experimental parasitic diseases.

Counterimmunoelectrophoresis↗
PubMedDate not supplied

[A critical study of immuno-enzymatic reactions coupled with the precipitation tests on cellulose acetate membranes].

Precipitating tests carried out on cellulose acetate membrane can be increased by treating the immune complexes with enzyme linked anti-immunoglobulin antibodies. From our trials in parastic diseases (Amoebiasis, schistosomiasis, fasciolasis, filariasis, hydatidosis, trichinosis) and mycosis (Aspergillosis, Candidiasis), it seems that the immuno-enzymatic labelling of the "active" precipitating reactions should only be taken in consideration. We must insit on the importance of ELIEDA (enzyme-linked-immuno-electrodiffusion-assay) and ELIDEPA (enzyme-linked-immuno-double-electro-phoresis-assay). Both of these analytical assays are particularly sensitive. The sequence of appearance of the multiple precipitating systems of the complex parasitic mosaic can easily be watched. The different classes of immunoglobulins and their kinetics are determined by the use of monospecific antibodies linked to different enzymes which give a polychromic specific staining.

Amebiasis↗
PubMedDate not supplied

[Filarial eosinophilic lung diseases, value of immunology (apropos of 9 cases)].

The study of nine cases of "eosinophilic lung" using specific immunological techniques indicates that the latter completes the geographical, clinical, radiological and hematological criteria of this syndrome. The search of circulating antibodies confirms the filarial etiology and individualises a remarkable immunological profile thus, suggesting a diagnosis in the case of occult filariases. The intensity of the immunological reactions brings a supplementary argument in favor of the hypothesis of a poor host-parasite relationship in the pathogeny of secondary manifestations to an infestation by W. bancrofti, B. malayi and other animal filariae such as B. pahangi.

Brugia↗
PubMedDate not supplied

[Study of humoral immunity in human biharziasis. Value of E.L.I.E.D.A. (enzyme-linked immunoelectrodiffusion assay)].

With a view to sensitize the immuno-electro-diffusion test (I.E.D.) and determine the immunoglobulins involved in the antigen-antibody reaction, the authors have improved the I.E.D. test by coupling it with an enzymatic technique. The precipitated immune-complexes are treated with antibodies conjugated with an enzyme specific for each class of immuno-globulins. This association realizes the E.L.I.E.D.A. (enzyme-linked immuno-electro-diffusion-assay). The sensitivity and specificity of the method are excellent, and should allow to follow the qualitative evolution of the bilharzian antibody in natural and experimental affections.

Antibodies↗
PubMedDate not supplied

[Serologic study of 51 cases of human trichinosis].

During the course of a recent epidemic of trichinosis two immunodiagnostic techniques were tested. Firstly indirect immunofluorescence (IF) using sections of the diaphram of infected mice and isolated trichine larvae. Secondly gel precipitation reactions, immunodiffusion and immunoelectrophoresis. It was observed that the induced specific antibodies appeared later than the clinical symptoms and the hypereosinophilia; the delay in appearance of the first positives detected being at least two to three weeks. Subsequently the titres rose rapidly and raised levels persisted for several months. Indirect immunofluorescence of excysted larvae remains the technique of choice, being rapid, sensitive and quantitative. The association of fluorescent and precipitation technique reduces the errors caused by cross reactions with other helminths.

Fluorescent Antibody Technique↗