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Biomedical subjects

J M Robson

Publications and source records attributed to J M Robson.

At least 37 records · Page 2Linked to original sources
PubMed1969

Anti-inflammatory properties of human inflammatory exudate.

An inflammatory exudate collected from a site of major surgery (partial gastrectomy) was found to possess definite anti-inflammatory properties when tested by the carrageenin oedema technique (a method widely adopted for the assessment of potential anti-rheumatic agents). Such anti-inflammatory properties could not be detected in the serum of normal healthy adults or in an abdominal asdtes fluid. The active component in the exudate showed several properties in common with a similar anti-inflammatory substance present in inflammatory exudates of animal origin.

Animals↗
PubMed1969

Partial purification of the anti-inflammatory factor(s) in inflammatory exudate.

1. The carrageenin foot test was established as a sensitive and reliable assay procedure for determining the anti-inflammatory activity of inflammatory exudates.2. Incubation alone at a temperature above 70 degrees C or with pronase at 37 degrees C destroyed the anti-inflammatory activity of exudate.3. The anti-inflammatory component of exudate was partially precipitated by 50% ammonium sulphate.4. A partial purification process was devised using Sephadex G-150 gel filtration and DEAE and CM cellulose ion exchange chromatography to obtain at least a 24 fold purification.5. Measurements of 11-hydroxycorticosteroid levels indicated that steroids were not involved in the mechanism by which the exudate produced its anti-inflammatory effects.

Animals↗