Biomedical subjects

J M Rose

Publications and source records attributed to J M Rose.

24 records · Page 2Linked to original sources

PubMed1985

Evidence that IFN-alph/beta induces two antiviral states active against different viruses.

IFN-alpha/beta has been suggested to require only one round of mRNA and protein synthesis to induce an antiviral state. We have examined the mechanism of induction of the antiviral states shown against three types of viruses: mengovirus (plus strand, sense RNA), vesicular stomatitis virus (VSV, minus strand RNA), and vaccinia virus (DNA). Mouse L cells were treated with IFN-alpha/beta and cycloheximide and then with actinomycin D on a schedule which allowed only one round of mRNA and protein synthesis. The cells were challenged with virus under single cycle growth conditions to determine the amount of antiviral activity against the particular challenge virus employed. These studies confirmed that most of the antiviral effect directed against VSV is achieved with one round of macromolecular synthesis. However, most of the antiviral effect directed against mengovirus and vaccinia virus seemed to require more than one round. These results suggest that IFN-alpha/beta induces two different antiviral states: one requiring one round of synthesis which is primarily responsible for the inhibition observed for VSV; and another requiring more than one round of synthesis which is primarily responsible for the inhibition observed for mengovirus and vaccinia virus.

Animals↗

PubMed1983

Profile of recovery after general anaesthesia.

The duration of impairment of mental functioning after anaesthesia was studied in 55 patients undergoing hernia repair who were divided into three groups in which the method of induction of anaesthesia (intravenous or inhalational) and ventilation (spontaneous or controlled) was varied. Performance in a five minute serial reaction time test and subjective estimates of coordination were assessed four times a day for two complete postoperative days and were compared with those in a control group of orthopaedic patients in hospital. After considerable impairment initially, reaction times in all groups gradually returned towards control values, but in patients breathing spontaneously during anaesthesia impairment recurred during the second postoperative day. These results suggest that such patients should be advised not to undertake hazardous tasks such as driving a car for at least 48 hours after a general anaesthetic. Discrepancies between subjective and objective assessments of impairment also suggest that patients should not rely on their own assessments of fitness to drive.

Anesthesia, General↗

PubMed1973

Degradation of methylmercury by bacteria isolated from environmental samples.

A total of 207 bacterial cultures, isolated from environmental samples, was screened for ability to degrade methylmercury. Of these, 30 were found positive for aerobic demethylation. Twenty-two of these were shown to be facultative anaerobes and 21 of these degraded methylmercury anaerobically. All positive species volatilized methylmercury aerobically, and methane was produced as a degradation product. Although methylmercury degradation was complete in most cases, material balances indicated some of the inorganic mercury formed was not volatilized and is presumed bound to the cells. All positive isolates were tolerant to at least 0.5 mug of methylmercury per ml, and the extent of volatilization of mercury increased with concentration to the threshold value. The results indicate that demethylating species are prevalent in the environment and may be important in suppressing the methylmercury content of sediments.

Aerobiosis↗

PubMed1966

Gamma-A-blood group antibodies.

The serological characteristics of gammaA-anti-A and anti-B were studied using, as a source, either colostrum, or fractions relatively rich in gammaA obtained from selected potent antisera. gammaA-anti-A and anti-B were never hemolytic nor did they sensitize red cells to agglutination by anticomplement globulin sera. gammaA-anti-A, like gammaG-anti-A and unlike gammaM-anti-A was unaffected by heating at 56 degrees C for 3 hr. On the other hand in the following three characteristics the behavior of gammaA fell between that of gammaG- or gammaM-anti-A: sensitivity to inactivation by 2-mercaptoethanol, ease of neutralization by A substance and degree of enhancement of agglutination in a medium of serum rather than saline. The agglutination produced by gammaA-anti-A was regularly enhanced by addition of anti-gammaA-globulin serum. In searching for gammaA-blood group antibodies of other specificities the following sera were tested: anti-D (32 examples); anti-c (2 examples); anti-Le(a) or -Le(b) (3 examples); anti-K (3 examples); anti-Fy(a) (3 examples), and anti-Jk(a) (3 examples). Only 3 sera, all containing anti-D, sensitized red cells to agglutination by anti-gammaA. There were no discrepancies between results obtained with four different anti-gammaA-globulin sera. Approximately half the sera were fractionated on DEAE-cellulose, and the fractions rich in gammaA tested for their ability to sensitize red cells to agglutination by anti-gammaA; no additional examples of gammaA-antibodies were detected. One of the three examples of gammaA-anti-D appeared in the serum of a woman during the course of deliberate reimmunization. gammaA-anti-D appeared only after three intravenous injections of red cells although the gammaG-anti-D titer rose considerably after a single injection. 3 yr after a fourth injection of Rh-positive cells gammaA-anti-D, as well as gammaG-anti-D, was still present in the serum.

Blood Group Antigens↗

PubMedDate not supplied

Endoscopic plantar fasciotomy: a retrospective analysis of results in 53 patients.

This retrospective study analyzes satisfaction of patients who had undergone an isolated endoscopic plantar fasciotomy (EPF) between January 1994 and January 1997. A subjective survey was completed and returned by 53 patients (a total of 69 feet), and a chart review was performed to determine final outcome. Postoperative follow-up averaged 7.2 months (range, 4-42 months). Postoperative pain levels were scored on a 7-point scale at 1 week, 1 month, and 6 months. Forty-three patients (81.1%) were satisfied with the EPF procedure and 10 patients (18.9%) were unsatisfied.

Adolescent↗

PubMedDate not supplied

Sliding oblique osteotomy for the treatment of hallux abducto valgus associated with functional hallux limitus.

This is a retrospective study of 27 patients (35 feet) with hallux abducto valgus associated with hallux limitus who underwent a sliding oblique osteotomy for surgical treatment between August 1997 and June 1998. Radiographic analysis and range-of-motion measurements were evaluated with an average follow-up of 65 days (range, 26-100). Preoperative criteria included < 45 degrees of dorsiflexion of the first metatarsophalangeal joint with weightbearing, no evidence of degenerative joint disease at the first metatarsocuneiform joint, and no previous surgical procedures on the first ray. The average preoperative intermetatarsal angle was 9 degrees, hallux abductus angle 17 degrees, and first metatarsal declination angle 15 degrees. The average postoperative intermetatarsal angle was 6.6 degrees, hallux abductus angle 10.3 degrees, and first metatarsal declination angle 21.7 degrees. Eighteen patients (22 feet) had a follow-up of over 6 weeks, and the first metatarsophalangeal joint was evaluated. The average gain in postoperative range of motion with weightbearing was 22.3 degrees.

Adult↗