Biomedical subjects
Publications and source records attributed to J M Ruíz.
We present an improved and simplified method for vital staining of the corneal endothelium, which allows high-quality images that are excellent for morphological and morphometric studies. This method is based upon the combination of trypan blue and alizarin red S. The main advantages of this technique are simplicity, rapidity and low cost.
An extracapsular cataract extraction was performed on both eyes of 24 rabbits. Irrigation for 10 min of the capsular bag of the left (test) eye with 8-12 cm3 of 1 litre of BSS-plus to which had been added 50 mg of 5-fluorouracil significantly inhibited proliferation of the anterior capsular epithelium as compared with the right (control) eyes identically treated except for the omission of 5-fluorouracil (p less than 0.001). Corneal endothelial counts did not differ significantly. A reduction in opacification of the posterior capsule is expected from 5-fluorouracil treatment.