Radiolabelled proline and hydroxyproline were separated on a C8 column (10 cm X 4.6 mm I.D.) with 10.4 mM sodium dodecyl sulphate in water-n-propanol (88:12, v/v) (pH 2.6) as the mobile phase at a flow-rate of 0.6 ml/min. The retention times of hydroxyproline and proline were 5 and 8 min, respectively. On-line radiometric detection was performed either in a homogeneous mode (liquid scintillator was added to the column effluent in the ratio 3.33:1) or in a heterogeneous mode (the detection cell was packed with a solid scintillator and 0.1 M ammonia was mixed with the column effluent in the ratio 1:6 in order to prevent adsorption of amino acids on the cell packing). Detection limits were in the range 100-900 dpm for individual isotopes and detection modes and the reproducibilities were better than 10%. The application of the method to a collagen synthesis study is reported.

Chromatography, High Pressure Liquid↗

PubMed1989

Dynamical theory of the conversion among the state multipoles in the collisions of hydrogenlike species with ions.

Explore the source record for details and available documents.

Journal Article↗

PubMed1989

[Results of a clinical trial of sulfasalazine in rheumatoid arthritis].

The authors tested the therapeutic effectiveness and tolerance of the sulphonamide chemotherapeutic preparation sulfasalazine in 69 patients with active rheumatoid arthritis. A one-year open clinical investigation was completed by 41 patients (59%) where in the course of treatment significant improvement of the majority of clinical and laboratory indicators of the activity of the rheumatic process was recorded. Treatment was terminated prematurely by 28 patients (41%) incl. 16 (23%) where treatment was discontinued because of undesirable side-effects which developed most frequently during the first months of treatment and were not very serious. X-ray evaluation of the joints of the hands after a one-year interval did not reveal deterioration of the X-ray findings in patients who completed the course of treatment.

Adult↗

PubMed1989

[The effect of particle size on the pharmacokinetics of piroxicam].

Two preparations of Piroxicam cps. (10 mg) VUFB Prague, prepared from crystalline and micronized substance, were compared. The release of the active ingredient into the solution in the medium of artificial gastric juice in vitro was examined. The results show that Piroxicam prepared from micronized substance corresponds to the preparation Felden cps. On the other hand, the release of the active ingredient from the preparation prepared from crystalline substance is lower. The pharmacokinetic parameters of Piroxicam from micronized substance are practically consonant to similar parameters of the preparation Felden cps. In Piroxicam prepared from crystalline substance there was a decrease in the rapidity of absorption, which, however, was not statistically significant in the group of volunteers under study. Other pharmacokinetic parameters were practically the same as in the other preparations described.

Adult↗

PubMed1988

Separation of type IX collagen from other cartilage collagens by hydrophobic interaction chromatography.

Collagen type IX was separated from other cartilage collagens (types II and XI) by hydrophobic interaction chromatography on a 25 cm X 8 mm I.D. stainless-steel column packed with Separon HEMA 1000 Bio. The mobile phase was 0.84 M ammonium sulphate with 0.1 M potassium dihydrogenphosphate (pH 6.5). Under these conditions only collagen type IX was eluted from the column; it could be monitored with UV detection (218 nm) or selectively with fluorescence detection (excitation 330 nm, emission filter 389 nm). The method can be used for the isolation and quantitation of collagen type IX. The assay was linear in the range 0-10 micrograms, the correlation coefficient was 0.99, precision 5.5% and accuracy 13%. The detection limit was about 0.6 microgram.

Animals↗

PubMed1988