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Biomedical subjects

J Macy

Publications and source records attributed to J Macy.

16 recordsLinked to original sources
PubMed1995

The effect of globe fixation on ablation zone centration in photorefractive keratectomy.

PURPOSE: For optimal vision, the ablation zone in photorefractive keratectomy should be centered over the entrance pupil. During ablation, the globe can be immobilized by the surgeon, with a suction ring around the corneoscleral limbus. Alternatively, the globe can be immobilized by patient fixation on a target, unassisted by the surgeon. We investigated which method results in better centration of the ablation zone over the entrance pupil, by using an objective, mathematical method to determine the ablation zone center. METHODS: Forty-eight eyes from 48 patients who underwent photorefractive keratectomy by the two techniques were studied retrospectively. The centers of the ablation zones were objectively determined by a weighted center of mass algorithm applied to the preoperative minus postoperative difference maps. The validity of the objective method was confirmed by comparison to subjective estimates of ablation zone centers made by independent human observers. RESULTS: The 19 eyes treated by surgeon fixation had an average decentration of the ablation zone of 0.63 +/- 0.31 mm (range, 0.01 to 1.00 mm), and the 29 eyes treated by patient fixation had an average decentration of 0.41 +/- 0.23 mm (range, 0.11 to 1.18 mm) (P = .027). CONCLUSIONS: The center of the ablation zone can be determined mathematically from the topographic map, to avoid observer bias. In this study, unassisted patient fixation during photorefractive keratectomy produced more accurate centration of the ablation zone than did surgeon fixation and has the potential for maximizing the quality of vision postoperatively.

Algorithms↗
PubMed1991

The use of Saturn II lenses in keratoconus.

We studied Saturn II contact lenses in keratoconus patients who were intolerant to other available contact lenses. A total of 24 patients, who were followed for up to 15 months, participated in the study. Visual acuities improved significantly following fitting with the Saturn II contact lens; whereas only one eye in the series was correctable to 20/20 before fitting, eight eyes were corrected to 20/20 following fitting. A great number of lenses had to be replaced during the study because of deposit formation and tearing at the interface (rate of replacement: 0.52 lenses per eye per month). A variety of complications occurred during lens wear, resulting in only six eyes wearing the Saturn II lens at the close of the study (out of 46 eyes that had started wearing the lens). Discomfort due to tightness of the peripheral segment was the most common cause of lens discontinuation. We conclude that the Saturn II lens was inadequate for fitting patients with advanced keratoconus.

Adolescent↗
PubMed1984

Anaerobiosis, formate, nitrate, and pyrA are involved in the regulation of formate hydrogenlyase in Salmonella typhimurium.

Three groups of mutants defective in the fermentative production of gas were isolated from Salmonella typhimurium LT2 subjected to transposition mutagenesis with Mu d(Apr lac). One group consisted of strains which lacked hydrogenase. The mutation site for this group was located in the vicinity of the known hyd gene. A second group consisted of mutants which lacked the formate dehyrogenase associated with hydrogenase. The mutation site was located in four of them. It was not in the vicinity of the previously described fhlD gene but was instead located at 93 min on the Salmonella map. The third mutant group, which consisted of strains that produced gas in triple sugar iron agar but not in nutrient agar supplemented with glucose, appeared to be pyrA mutants. The insertion site was located in the vicinity of pyrA , and they required arginine and pyrimidines for growth. Expression of the lac operon in the hyd mutants was induced by anaerobiosis. It was only slightly increased by the addition of formate under anaerobic conditions and slightly decreased by the addition of nitrate. Nitrate had no effect in an hyd ::Mu d strain that also carried a chlC::Tn10 insertion. Full expression of the lac operon in the fhl mutants required both formate and anaerobic conditions. The presence of nitrate in addition to formate resulted in activities about half those obtained in its absence, even in the fhl ::Mu d chlC::Tn10 double mutant. In the absence of formate, nitrate reduced expression only in the fhl ::Mu d single mutants. Expression of the lac operon among the pyrA mutants was repressed by arginine and cytosine and also by anaerobiosis. An explanation for the involvement of pyrA in aerobic and anaerobic energy metabolism is proposed.

Aerobiosis↗
PubMed1977

The prediction of fetal oxygenation by an on-line computer analysis of fetal monitor output.

The deceleration delay index (DDI) is a statistical parameter computed by an on-line system which analyzes fetal monitor output for the temporal relationship between each uterine contraction and any associated deceleration of the fetal heart rate. A similar parameter describing this relationship is known to be quantitatively predictive of fetal oxygenation in the rhesus monkey, a species for which the level of fetal oxygenation is also known to be directly and progressively related to aberrations in the vital signs, to brain damage, and to fetal death. Thus, the DDI should be of significant, practical value in predicting fetal hypoxia at a time early enough to avert severe neonatal depression and/or hypoxic brain damage. A blind clinical trial of 106 parturients is presented which supports this concept by showing the DDI to be highly correlated with another oxygenation-associated parameter, the one-minute Apgar score.

Apgar Score↗
PubMed1975

Evidence for cytochrome involvement in fumarate reduction and adenosine 5'-triphosphate synthesis by Bacteroides fragilis grown in the presence of hemin.

Growth of Bacteroides fragilis subsp. fragilis on glucose was very much stimulated by the addition of hemin (2 mg/liter) to the medium. The generation time decreased from 8 to 2 h, and the molar growth yield increased from YM = 17.9 to YM = 47 g (dry weight) of cells per mol of glucose. In the absence of hemin, glucose was fermented to fumarate, lactate, and acetate. The cells did not contain detectable amounts of cytochromes or fumarate reductase. In the presence of hemin, the major products of fermentation were succinate, propionate, and acetate. A b-type cytochrome, possibly a c-type cytochrome, and a very active fumarate reductase were present in the cells. It is concluded from these results that hemin is required by B. fragilis to synthesize a functional fumarate reductase and that the hemin-dependent, enormous increase of the growth yield may be due to adenosine 5'-triphosphate production during reduction of fumarate to succinate.

Acetates↗