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Biomedical subjects

J Martinis

Publications and source records attributed to J Martinis.

11 recordsLinked to original sources
PubMed1983

Novel applications of monoclonal antibodies.

The ability to "engineer" antibodies, using the techniques of somatic cell genetics and cell fusion, has opened a new era in immunochemistry. No longer are immunologists, limited by the vagaries of polyclonal antibody production. Using hybridoma technology, consistent preparations of precisely defined monoclonal antibodies are now available. Immunochemists are free to pick and choose those precisely defined characteristics of a homogeneous species of antibody that are best suited for a given application. To date, monoclonal antibodies have been used primarily as research chemicals. However, the implications of this new technology for diagnostic, therapeutic, and separation systems are rapidly becoming apparent.

Acid Phosphatase↗
PubMed1981

Monoclonal antibodies in clinical immunology.

Advances in clinical immunodiagnostics resulting from the application of hybridoma technology are starting to appear. Monoclonal antibodies are beginning to displace their antiquated progenitors, polyclonal antisera, in many facets of immunology. Their homogeneity, specificity, and availability make hybridoma-derived antibodies the immunological reagents of the future in immunoassays, immuno-affinity chromatography, immunotherapy, and areas yet to be defined.

Antibodies, Monoclonal↗
PubMed1979

Chromosomal location of the genes for human immunoglobulin heavy chains.

We have studied somatic cell hybrids between P3x63Ag8 mouse myeloma cells deficient in hypoxanthine phosphoribosyltransferase (EC 2.4.2.8) and either human peripheral lymphocytes or human lymphoblastoid or myeloma cells for the production of human immunoglobulin chains and for the expression of enzyme markers assigned to each of the different human chromosomes. Human chromosome 14 was the only human chromosome present in all independent hybrids producing mu, gamma, and alpha human heavy chains. In two of the independent hybrids that produced human heavy chains, human chromosome 14 was the only human chromosome present in the hybrid cells. Loss of human chromosome 14 from these hybrids resulted in the concomitant loss of their ability to produce human immunoglobulin heavy chains. In view of these results, we conclude that the genes for human immunoglobulin heavy chains are located on human chromosome 14 in immunoglobulin-producing human cells.

Animals↗
PubMed1978

Somatic cell hybrids producing antibodies specific for the tumor antigen of simian virus 40.

We have produced somatic cell hybrids between mouse myeloma cells deficient in hypoxanthine phosphoribosyltransferase IMP: pyrophosphate phosphoribosyltransferase; EC 2.4.2.8) and spleen cells derived from mice primed with either syngeneic or allogeneic cells transformed by simian virus 40. Such hybrids produced antibodies specific for simian virus 40 tumor (T) antigen. Only four of twelve independent hybrid cell cultures produced antibodies against simian virus 40 T antigen that crossreacted with the T antigen induced by BK virus, a human papovavirus isolated from patients who had undergone immunosuppressive therapy.

Animals↗