PubMed Health⌕ Search

Biomedical subjects

J Matousek

Publications and source records attributed to J Matousek.

At least 73 records · Page 4Linked to original sources
PubMed1979

Effect of native and modified bull seminal ribonuclease on tumour and testicular cells and phytohaemagglutinin-stimulated pig lymphocytes.

The carboxymethylated, oxidized and reduced forms of AS RNase inhibited transplantability and DNA synthesis of tumour cells BP-8 and EL-4 incubated in vitro. With tumour cells EL-4 the results under in vitro conditions did not not correspond to those obtained under the conditions in vivo. The survival of mice given injections of EL-4 cells and of the native and carboxymethylated AS RNase was only slightly prolonged. Mice that received intra-abdominally BP-8 cells and both carboxymethylated and oxidized and reduced forms of AS RNase survived two or three times longer than the controls. Succinylation and maleylation of AS RNase eliminated any antitumoral effect. Aspermatogenic activity of AS RNase was abolished by any modification of the molecule which had substantially reduced, or removed, the RNase activity. Neither native nor modified forms of AS RNase had an inhibitory effect on unstimulated pig lymphocytes. The DNA synthesis of PHA-stimulated lymphocytes was inhibited by the native and carboxymethylated AS RNase only. Bovine pancreatic A RNase had any inhibitory effect on neither tumour nor testicular cells.

Animals↗
PubMed1977

Action of bull seminal vesicle ribonuclease on mouse leukaemic cells BP-8 and EL-4.

Bull seminal vesicle ribonuclease, when added in vitro to the suspension of ascitic leukaemic cells EL-4 and BP-8 for 2 hours at 37 degrees C and then transplanted to the abdominal cavities of mice, inhibitied the proliferation of these cells in vivo. When the temperature of the medium was decreased to 25 degrees C, AS RNase was bound to the leukaemic cell surfaces, but it exerted no inhibitory effect on BP-8 cells and showed a partial effect on EL-4 cells even after 24 hours. AS RNase administered to mice with leukaemic cells in diffusion chambers significantly decreased the number of cells inside the chambers as compared to the controls. The number of ascites cells was also significantly reduced after the injection of AS RNase for 1 week into mice with leukaemic cells implanted in the abdominal cavities. The reduction of cells in ascitic fluid did not cause a significant prolongation of survival of experimental mice. Only mice with transplanted BP-8 cells had significantly less cells in ascitic fluid and their survival was prolonged to 48 days in comparison with 25 days' survival in control mice after administration of pure AS RNase. In addition, AS RNase depressed the growth of solid tumours BP-8 and EL-4. AS RNase did not change the weight of body organs except the testes. Absorption experiments showed increased absorption of AS RNase by leukaemic cells BP-8 and EL-4 and by testes and spleen homogenates as compared with other body organs.

Animals↗
PubMed1977

Action of A RNase and AS RNase on growth of cells in vitro.

The effect of bovine pancreatic A RNase and bull seminal vesicle AS RNase on proliferation of HeLa cells and LEP cells in vitro was studied. The ribonucleases were used in doses of 0.1, 1.0, 10.0, and 100.0 microgram per ml of medium and 100 000 inoculated cells. Proliferation was evaluated by the growth curves. Both in single and long-term experiments. A RNase in all doses used had no effect on cell proliferation, whereas AS RNase exerted inhibitory activity beginning with the dose of 10 microgram, and affected more markedly the LEP cells (a diploid non-malignant cell line) than the malignant heteroploid HeLa cells. When added to the cell suspension, AS RNase was shown by indirect immunofluorescence to bind to HeLa cell membranes in all phases of growth, whereas AS RNase was found on membranes of LEP cells only when they were derived from the log phase of growth.

Animals↗