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Biomedical subjects

J McCutchan

Publications and source records attributed to J McCutchan.

3 recordsLinked to original sources
PubMed1998

[Integrated use of psychotherapeutic treatment methods in therapy of alcoholism].

The treatment of alcoholism is more promising than commonly assumed. Its success is based on the acceptance of a long-term treatment concept over a period of approximately 2 years, the willingness to differentiate between the individual treatment courses according to their underlying individual psychopathologies as well as adapting treatment measures to the actual phases during restitution. Many years of experience with various psychotherapeutic methods have proven that not so much one certain method, but their integrative application depending on the individual situation is relevant to treatment success. Thus, during treatment, a change between supportive, confrontative, systemic and family therapy-oriented elements can occur.

Alcoholism↗
PubMed1989

Construction of an Agrobacterium tumefaciens C58 recA mutant.

Clones encoding the recA gene of Agrobacterium tumefaciens C58 were isolated from a cosmid bank by complementation of an Escherichia coli recA mutation. Subcloning and mutagenesis with the lacZ fusion transposon Tn3HoHo1 located the Agrobacterium recA gene to a 1.3-kilobase segment of DNA. beta-Galactosidase expression from the fusions established the direction in which the gene was transcribed. The gene restored homologous recombination as well as DNA repair functions in E. coli recA mutants. Similar complementation of DNA repair functions was observed in the UV-induced Rec- Agrobacterium mutant, LBA4301. The Agrobacterium recA gene was disrupted by insertion of a cassette encoding resistance to erythromycin, and the mutated gene was marker exchanged into the chromosome of strain NT-1. The resulting strain, called UIA143, was sensitive to UV irradiation and methanesulfonic acid methyl ester and unable to carry out homologous recombination functions. The mutation was stable and had no effect on other genetic properties of the Agrobacterium strain, including transformability and proficiency as a conjugal donor or recipient. Furthermore, strain UIA143 became tumorigenic upon introduction of a Ti plasmid, indicating that tumor induction is independent of recA functions. Sequence homology was detected between the recA genes of strain C58 and E. coli as well as with DNA isolated from agrobacteria representing the three major biochemically differentiated biovars of this genus. In some cases, biovar-specific restriction fragment length polymorphisms were apparent at the recA locus.

DNA Mutational Analysis↗