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Biomedical subjects

J Mele

Publications and source records attributed to J Mele.

6 recordsLinked to original sources
PubMed2001

Changes in expression of antioxidant enzymes affect cell-mediated LDL oxidation and oxidized LDL-induced apoptosis in mouse aortic cells.

Transgenic mice overexpressing Cu/Zn superoxide dismutase (hSod1Tg(+/0)) or catalase (hCatTg(+/0)) and knockout mice underexpressing manganese superoxide dismutase (Sod2(+/)(-)) or glutathione peroxidase-1 (Gpx1(-/-)) were used to study the effect of antioxidant enzymes on cell-mediated low density lipoprotein (LDL) oxidation and oxidized LDL (oxLDL)-induced apoptosis. Incubation of LDL with mouse aortic segments or smooth muscle cells (SMCs) resulted in a significant increase in LDL oxidation. However, LDL oxidation was significantly reduced when LDL was incubated with aortic segments and SMCs obtained from hSod1Tg(+/0) and hCatTg(+/0) mice compared with those obtained from wild-type mice. In contrast, LDL oxidation was significantly increased when LDL was incubated with aortic segments and SMCs obtained from Sod2(+/)(-) and Gpx1(-/-) mice. CuSO(4)-oxidized LDL increased DNA fragmentation and caspase activities in the primary cultures of mouse aortic SMCs. However, oxLDL-induced DNA fragmentation and caspase activities were reduced 50% in SMCs obtained from hSod1Tg(+/0) and hCatTg(+/0) mice compared with wild-type control mice. In contrast, oxLDL-induced DNA fragmentation and caspase activities were significantly increased in SMCs obtained from Sod2(+/)(-) and Gpx1(-/-) mice. These findings suggest that overexpression of Cu/Zn superoxide dismutase or catalase reduces cell-mediated LDL oxidation and oxLDL-induced apoptosis, whereas underexpression of manganese superoxide dismutase or glutathione peroxidase-1 increases cell-mediated LDL oxidation and oxLDL-induced apoptosis.

Animals↗
PubMed2000

Evaluating ambulatory resident practice using an Internet-based system.

To improve faculty participation in providing feedback on resident outpatient practice, we designed and implemented an Internet-based evaluation system. Attending physicians scored residents on 7 questions related to specific practice behaviors, and completed a free-text comment section. Between January and June 1998, 516 evaluations for 107 residents were generated using the Internet-based system, compared with 29 handwritten evaluations for 82 residents between January and June 1997. The number of faculty evaluations was 527 for 121 residents in the second half of 1998. An Internet-based evaluation system can improve faculty compliance with performing evaluations.

Adult↗
PubMed1998

Will commercial managed care patients accept residents as their primary care providers?

We conducted a telephone survey of patients in a university-based medical practice to determine if there was a difference across payer class in patients' willingness to have supervised housestaff physicians function as their primary care providers. Overall, commercial managed care patients were more likely to object to seeing housestaff physicians than were Medicaid or Medicare patients (50% vs 32% or 23%, respectively). However, prior outpatient care by a resident physician significantly increased patient willingness to be cared for by a resident. This effect of prior care by a resident was noted in the managed care as well as the Medicaid and Medicare populations. Although there may have been self-selection, our data demonstrate that a significant proportion of managed care patients who have had residents as their primary care providers are amenable to continuing this practice.

Aged↗
PubMed1995

The effect of antenatal surgery on craniofacial growth in sheep model.

The purpose of this investigation was to evaluate the effect of antenatal palatal surgery in a sheep model. At 75 to 90 days of gestation, 20 fetuses underwent surgical in utero manipulation of their palates with the incision and elevation of bilateral bipedicled mucoperiosteal flaps. Sixteen of the 20 sheep (80 percent) went on to a normal full-term delivery. The palates and facial appearances were grossly evaluated, and detailed measurements were made of the palates. The data underwent statistical analysis. We were unable to discern any differences between the experimental group and the age-matched controls. Antenatal palatal surgery appeared to have no effect on craniofacial growth and development.

Animals↗
PubMed1988

Case-control study of diet and mesothelioma in Louisiana.

Data were analyzed from a case-control interview study of malignant mesothelioma in Louisiana, which gathered information on usual diet and on lifetime occupational exposure to asbestos. Thirty-seven patients with malignant mesothelioma of the pleura (n = 32) or peritoneum (n = 5) were matched to controls according to age, sex, race, and factors related to case ascertainment (hospital and date of diagnosis, or parish and date of death). Twenty-one of the 37 cases were judged by masked occupational review to have been exposed to asbestos (57%), compared to seven of 37 controls (19%). Seven additional cases and 10 additional controls had occupational histories suggestive of asbestos exposure. With regard to usual diet before illness, cases reported less frequent consumption of homegrown produce (p = 0.005), cruciferous vegetables (p = 0.005), and all vegetables combined (p = 0.09) than did the controls. An estimate of usual carotene intake was also significantly lower in cases (p = 0.03). Dose-dependent reductions in risk were seen with increasing consumption of vegetables, especially cruciferous vegetables (p for trend = 0.013). These associations were not explained by differences in asbestos exposure as measured by the occupational review. The results indicate that consumption of vegetables or some vegetable-related constituent may have a protective effect on developing mesothelioma.

Adult↗