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Biomedical subjects

J Menage

Publications and source records attributed to J Menage.

26 records · Page 2Linked to original sources
PubMed1989

Biochemical and structural properties of the cartilage end-plate and its relation to the intervertebral disc.

The cartilaginous end-plate is thought to play an important role in the nutrition of the intervertebral disc, and hence may be of significance in the etiology of back pain. The present study describes the biochemical and histologic properties of the end-plate and adjacent tissues in the young human adult lumbar spine. Thus, a datum is established in which to demonstrate variation with location and relationships between properties of different tissues. Results of the chemical analyses show a change in composition through the end-plate with that at the outer annulus and nearer the bone, having higher collagen but lower proteoglycan and water contents, than the end-plate nearest the disc at the nucleus. Histology demonstrated numerous disruptions along the end-plate, with Schmorl's nodes being present in several specimens. Where these occurred, the disc, and in some cases the end-plate, showed loss of proteoglycan compared with the surrounding tissue.

Adolescent↗
PubMed1982

Osteogenesis in osteopetrotic mice.

Ectopic bone arising in grafts of compatible normal intact bone marrow in microphthalmic osteopetrotic recipients was examined in the light microscope and was found to be unaffected by the deficiency that curtails resorption of primitive woven bone in osteopetrotic animals.

Animals↗
PubMed1982

Tissue repopulation during cure of osteopetrotic (mi/mi) mice using normal and defective (We/Wv) bone marrow.

Resorption of petrotic bone in osteopetrotic (mi/mi) mice was brought about by transplantation of bone marrow to X-irradiated recipients. In an attempt to learn more about the donor cell line involved in this process, both normal and defective marrow were used. The consequent repopulation of the lympho-myeloid complex was monitored by isoenzymes of glucose phosphate isomerase. The progress of normal marrow grafts was contrasted with that of a defective marrow (We/Wv). Despite the observation with We/Wv marrow showed reduced ability to form colonies in the spleen of an irradiated recipient, this marrow was as effective as normal marrow in inducing resorption of petrotic bone. The primordial stem cell for the osteoclast (haematopoietic stem cell?) is thus not a CFUS. Chimaeras with resolution of osteopetrosis by We/Wv bone marrow may exhibit erythropoiesis from residual stem cells of the host but leucocytes and platelets from the donor.

Anemia, Macrocytic↗
PubMed1981

The fate of allogeneic grafts of intact bone marrow in immunologically tolerant recipients and after abrogation of the tolerance.

In ossicles derived from grafts of compatible intact bone marrow in the subcapsular renal site the ectopic bone remains of donor provenance but the haemopoietic elements are partially replaced with tissue by host cells derived from stem cells migrating from the blood. Ossicles derived from incompatible intact bone marrow grafted in immunologically tolerant recipients are morphologically identical to those derived from compatible intact bone marrow. On abolition of the tolerance the allogeneic ectopic bone and donor microenvironment for haemopoiesis became manifest: haemopoietic marrow, even the host elements, was rapidly lost, bone disappearing rapidly if recently established, slowly if long established. Provided the state of immunological tolerance persists, the histoincompatible bone and microenvironment of the donor co-exist with marrow derived from the circulating haemopoietic stem cells of the tolerant recipient.

Animals↗
PubMed1979

Resolution and relapse of osteopetrosis in mice transplanted with myeloid tissue of variable histocompatibility.

Osteopetrotic microphthalmic mice (mi/mi) were treated by injections of suspensions of myeloid tissue, newborns i.p., and weanlings i.v. Donated syngeneic material effected permanent cure of oteopetrosis provided that the dose was large enough (10(8) cells of bone marrow). H-2-compatible allogeneic bone marrow was initially as effective, but relapse ensued in immunocompetent mice. H-2-incompatible marrow was ineffecitve except in one set of newborn tolerant mice. Total body X-radiation in sublethal doses to recipients allowed permanent cure with H-2-compatible, and, in one circumstance, with H-2-incompatible marrow in smaller doses. The best results were obtained after lethal irradiation and the smaller dose of marrow. Results were checked by chromosome assay demonstrating that cure or relapse was correlated with permanent take or rejection, respectively, of a transplant in a recipient's bone marrow. Retention of donor lymphocytes alone was not associated with effective bony resorption; the candidate cell line for effectiveness remains the haematopoietic stem cell-monocyte-tissue phagocyte.

Animals↗
PubMedDate not supplied

Type III collagen in the intervertebral disc.

Several collagen types have now been isolated from the intervertebral disc, although type III collagen has previously only been extracted from human pathological disc. In this study, type III collagen has been isolated from normal human and bovine intervertebral disc and immunolocalized in sections of rat, sheep, bovine and 'normal' human intervertebral disc of various ages. Staining with antisera to type III collagen is localized primarily around the cells. Results indicate that cells of the disc sit in 'chondrons', similar to those seen in the deep and mid zones of articular cartilage. We suggest that type III collagen is present in the intervertebral disc and hypothesize that it may be involved in the organization of the pericellular environment, perhaps linking the chondron capsule to the interterritorial matrix.

Animals↗