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J Michaelson

Publications and source records attributed to J Michaelson.

29 records · Page 2Linked to original sources
PubMed1981

Further biochemical data on Qa-2.

The Qa-2 differentiation alloantigen is coded by a gene situated between the D and Tla loci of the murine major histocompatibility complex (H-2). Qa-2-bearing protein was isolated by immunoprecipitation and found to be composed of subunits of 40 000 and 12 000 daltons by SDS polyacrylamide gel electrophoresis (PAGE). The 12 000 dalton material was identified as beta 2-microglobulin (beta 2M) by its molecular weight (SDS PAGE), charge (isoelectric focusing), antigenicity (reactivity with xenogenic anti-beta 2M), and genetics. The 40 000 dalton mol. wt. of Qa-2 heavy chains is 5 000 daltons less than that of D and K molecules (45 000 daltons). The quantity of Qa-2 isolated by immunoprecipitation was found to vary in strain-specific fashion and as much as a 15-fold difference was observed.

Animals↗
PubMed1980

H-2-controlled polymorphism of the gamma chain of Slp (sex-limited protein).

A genetic polymorphism detected by the O'Farrell two-dimensional technique (isoelectric focusing and SDS-PAGE) of the murine sex-limited protein (Slp) is described and shown to map to the H-2 complex. The Slp charge variation was found to be in the gamma chains. Inbred strains carrying the H-2w7 and H-2wr7 haplotypes, which are derived from a wild mouse, had Slp-gamma chains with pI = 6.55 (Slp-1b). All other inbred strains, bearing H-2j,H-2s,H-2p,H-2d,H-2u, as well as three additional Slp-constitutive wild females captured in Chile, had Slp-gamma chain with pI = 6.71 (Slp-1a).

Animals↗
PubMed1979

A cell surface antigen, TER, expressed by embryos and germ cells.

An antiserum prepared in rabbits against the C3HeB/FeJ mouse ovarian teratocarcinoma E6496 was absorbed in vivo in C3HeB/FeJ mice. This absored antiserum identified an antigen, denoted TER, that is present on sperm, ova, embryonic germ cells, and cells of the early mouse embryo. TER was absent from all adult somatic cells tested, but found on several murine tumors.

Absorption↗
PubMed1977

Molecular similarities between the Qa-2 alloantigen and other gene products of the 17th chromosome of the mouse.

The alloantigen Qa-2, whose gene is located on the 17th chromosome between H-2D and Tla, is identified as a molecule of 43,000 daltons which is associated with beta 2-microglobulin. Qa-2 comprises approximately 0.15% of the iodinateable cell surface protein of lymph node cells. Sequential precipitations demonstrated that Qa-2 is distinct from H-2D and H-2K molecules.

Animals↗
PubMed1974

Evaluation of bis-benzimidazoles in the treatment of murine lymphocytic choriomeningitis virus infections.

Seventy percent of the mice receiving (S,S)-1,2-bis(5-methoxy-2-benzimidazolyl)-1,2-ethandiol (A36683) in their drinking water lived at least four times longer than control mice when infected with 10 or 100 mean lethal doses of lymphocytic choriomeningitis virus strain UBC. In the next 4 months, most of the survivors died with lymphocytic choriomeningitis-like symptoms. Drug treatment during the first 7 days after infection was found to have no significant effect on virus titers in various organs. The sparing effect of the drug is discussed in terms of immunosuppression.

Animals↗
PubMedDate not supplied

Allogeneic reactivity in normal mouse serum.

Normal C57BL/6 (B6) mouse serum was tested in the direct cytotoxicity assay for specific reactivity against lipopolysaccharide (LPS)-stimulated mouse spleen cells. Selective reactivity was found in weanling and adult serum against lymphoblasts from mice that express an antigen encoded by the H-2Kk region of the major histocompatibility complex (MHC). Other strains, congenic with B6 at the MHC, did not exhibit the same alloreactivity. Serum from mice of a congenic strain being derived in our laboratory, which differs from B6 at two unlinked loci, Tla and nu, exhibited similar reactivity against the B6-H-2k LPS-stimulated lymphoblasts, implying that a competent T cell compartment is not necessary for generation of this reactivity. Such reactivities may result from environmental stimulation of the immune system, from internal immunoregulatory controls, or from some combination of these immune stimuli.

Animals↗