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Biomedical subjects

J Mikucki

Publications and source records attributed to J Mikucki.

At least 55 records · Page 3Linked to original sources
PubMed1978

Coagulase in starved Staphylococcus aureus.

The resting cells of S. aureus strain Smith (diffuse) lost marked amount of free amino acids and proteins during their starvation. The starved cells contained less coagulase and that enzyme was released into the medium during starvation. After transfer into nutrient medium, those cells produced less coagulase than the non-starved ones. The restoration of amino acid pool resulted in coagulase production on initial level.

Amino Acids↗
PubMed1977

Factors influencing L-asparaginase production by staphylococci.

Cultural and nutritional requirements for a maximum synthesis of 1-asparaginase by staphylococci were determined. The best production of the enzyme was found in the stationary phase of growth of a batch culture. The highest 1-asparaginase yield was obtained when the culture were aerated during an exponential phase of growth and further incubated in the stationary phase. Optimum pH for the enzyme production was 7.5. Glucose inhibited the enzyme formation. Maximum yield of 1-asparaginase was obtained when casein hydrolysate and yeast extract were supplied as carbon and nitrogen sources. Repression by 1-asparagine and 1-aspartic acid was absent.

Air↗