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J Milburn

Publications and source records attributed to J Milburn.

6 recordsLinked to original sources
PubMed2001

Beyond the genome: turning data into knowledge.

Since the publication of the draft sequence of the human genome in April 2001 (1,2), the pressure on life scientists to annotate and gain useful knowledge from the millions of As, Gs, Cs and Ts, is ever increasing. Now that the initial hype has passed, the life sciences field demands the technologies and computer power that can produce and analyze data in a high-throughput manner for drug discovery and disease diagnosis. Cambridge Healthtech Institute's Beyond Genome 2001 conference(1) (17-22 June 2001, San Francisco, CA, USA) brought together the latest research in these technologies in a 'tri-conference' that encompassed the 10th Annual Conference of Bioinformatics and Genome Research, the 3rd Annual Conference of In Silico Biology and the 5th Annual Conference of Proteomics.

Journal Article↗
PubMed1985

Adenylate cyclase activity in neocortex and fetal zone membrane fractions of the human fetal adrenal gland.

Whether peptide hormones other than ACTH may be responsible for the difference in size or rate and pattern of steroidogenesis of the fetal zone (FZ) compared to those of the neocortex (NC) of the human fetal adrenal gland is controversial. In the present investigation, the activity of adenylate cyclase in membrane fractions of separated zones of the human fetal adrenal gland was determined. Basal adenylate cyclase activity was 2- to 3-fold greater in NC than in FZ membrane fractions. The addition of ACTH-(1-24) stimulated adenylate cyclase activity in both zones, but the activity was more sensitive to ACTH (10(-10) M) in NC fractions than in FZ fractions (10(-7) M). In addition to ACTH-(1-24), the effect of other ACTH-related peptides on the activity of adenylate cyclase in the separated zones of the adrenal gland was investigated. 16K fragments 2-36, gamma 3MSH, alpha MSH, beta-endorphin, leu-enkephalin, and met-enkephalin, as well as hCG, FSH, prostaglandin E2, prostaglandin F2 alpha, epinephrine, and norepinephrine did not stimulate adenylate cyclase activity in either zone. It is concluded that basal and ACTH-(1-24)-stimulated adenylate cyclase activities are greater in NC than in FZ membrane fractions. In addition, the results of the present investigation do not support the concept that other ACTH-related peptides or peptide or protein hormones increase steroidogenesis by stimulating adenylate cyclase activity in the human fetal adrenal gland.

Adenylyl Cyclases↗