PubMed Health⌕ Search

Biomedical subjects

J Millet

Publications and source records attributed to J Millet.

78 records · Page 5Linked to original sources
PubMedDate not supplied

Heterologous hybridization of bacterial DNA to the endoglucanases A and B structural genes celA and celB of Clostridium thermocellum.

DNA from various cellulolytic and non-cellulolytic bacteria was found to hybridize to Clostridium thermocellum NCIB10682 DNA fragments carrying the structural genes celA and celB which code for endoglucanases A and B. Homology to celA was detected in Agrobacterium rhizogenes, Azospirillum brasilense, Bacillus subtilis, Cellulomonas sp., Clostridium stercorarium, Erwinia chrysanthemi, Pseudomonas solanacearum and Streptomyces griseus. Homology to celB was detected only in B. subtilis, C. stercorarium and E. chrysanthemi. No homology to celA or celB was detected in Agrobacterium tumefaciens, Rhizobium japonicum, Rhizobium meliloti, Rhizobium ORS571 and Trichoderma reesei. Hybridization performed with the homologous strain NCIB10682 and with C. thermocellum LQRI suggested that the two strains were identical in terms of cel genes. In addition, it is likely that the C. thermocellum cel genes, whose number is presently estimated to be at least ten, do not belong to a gene family, since heterologous hybridization was observed only to a single small EcoRI or HindIII fragment homologous to celB.

Bacteria↗
PubMedDate not supplied

The beneficial effect of a beta-D-xyloside, Iliparcil, in the prevention of postthrombolytic rethrombosis in the rat.

The effect of Iliparcil, a new orally active beta-D-xyloside venous antithrombotic, was studied on the rethrombosis following thrombolytic therapy in rats, using a modified Umetsu model. The drug was administered by oral route prior to thrombolytic therapy, which consisted of administering a combination of heparin and urokinase (H/U) at 37.5 and 70,000 IU/kg, respectively. Time to reocclusion increased from 3.9 min with saline to 10.5 min following H/U injection. When Iliparcil (30 mg/kg, oral route) was administered 4 h before H/U injection, the time to reocclusion was increased by 250% compared with H/U alone (p < 0.001). Similarly, dermatan sulfate (DS), administered intravenously (3 mg/kg) 5 min before thrombus induction, also increased the time to reocclusion (300% compared with H/U alone; p < 0.001). It was also shown that times to reocclusion following Iliparcil or DS treatments were still increased even when heparin dosage was decreased. These results suggest that an antithrombotic product derived from the beta-D-xyloside family could be advantageously used in combination with thrombolytic treatment instead of heparin, which causes complications and side effects.

Animals↗
PubMedDate not supplied

[Improvement of blood filtrability with a purified extract of black currant anthocyanosides in cynomologus monkeys on a fat diet].

A fat diet has been administered for 3 months to Cynomologus monkeys treated with blackcurrant anthocyanosides as compared with others receiving no treatment. Blood filtrability of animals given a fat diet alone was significantly lowered (20.8%; p less than 0.01) as compared with that of animals receiving a standard diet. Blood filtrability of animals receiving a fat diet plus blackcurrant anthocyanosides was not significantly different from that of animals given a standard diet. Emphasis is laid on a new approach to the action of this type of product on blood vessel contents as compared with the known action on the container.

Animals↗
PubMedDate not supplied

[Serological relationships between serylprotease and esterase excreted by "Bacillus subtilis" during sporulation (author's transl)].

During the sporulating phase of Bacillus subtilis an extracellular serylprotease (SPE) and an esterase are excreted. The two enzymes follow the same kinetics of appearance in the culture medium and have some properties in common, in particular both enzymes hydrolyze benzoyltyrosine ethylester and are inhibited by phenylmethylsulfonyl fluoride. This raised the question of a possible structural relationship between the two proteins. A specific antiserum against the SPE was prepared. Using a radioimmunological assay, no reaction with the esterase could be detected. Asporogenous mutants (SpoOA and SpoOB) imparied in the production of both enzymes were analysed. Immunological assays with SPE-specific antiserum supported the hypothesis that the regulation of the biosynthesis of both enzymes would be affected by SpoOA and SpoOB mutations.

Antibody Specificity↗