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J Mitsuhashi

Publications and source records attributed to J Mitsuhashi.

27 records · Page 2Linked to original sources
PubMed1980

Uptake of amino acids and glucose by microfilariae of Dirofilaria immitis in vitro.

The uptake of amino acids and glucose by microfilariae of Dirofilaria immitis maintained for 8 days in vitro was studied. The quantity of glutamic acid, histidine, lysine, alanine, tryptophan, and cystine in the medium did not change. Isoleucine, serine, valine, phenylalanine, tyrosine, methionine, and cystathionine decreased slightly; both asparatic acid and leucine decreased considerably. Glutamine, threonine, and proline decreased markedly, while glycine increased to some extent. Ornithine increased and arginine decreased; however, their changes were smaller than the changes of respective amino acids in the control medium free of microfilariae. Glucose decreased markedly.

Amino Acids↗
PubMedDate not supplied

Vitamin requirements of the cultured flesh fly cells, Sarcophaga peregrina (Diptera, Sarcophagidae).

Essential vitamins for the growth of a cell line derived from the flesh fly, Sarcophaga peregrina, were determined. By examining the survivability of continuous passages of the cells in the chemically defined medium lacking one vitamin, thiamine, riboflavin, pantothenate and either niacin or niacinamide were found to be essential for the continuous growth of the flesh fly cells in vitro. [Originally published in Volume 37, Archives of Insect Biochemistry and Physiology, 37:283-286 (1998).]

Animals↗
PubMedDate not supplied

Production of rotenone-inactivating substance(s) by rotenone-resistant insect cell line.

In a previous paper, we showed that a cell line derived from hemocytes of the cabbage armyworm, Mamestra brassicae (R-cell) was a thousand times as resistant to rotenone as that from ovaries of the same species (S-cell). The S-cells were killed by rotenone at concentrations higher than 10(-9) M, while R-cells at higher than 10(-6) M. When the R-cells were cultured in the medium containing 10(-9) M rotenone, the ability of rotenone to kill the S-cells was lost in the used medium. Also, when rotenone was incubated in the medium conditioned with R-cells, it lost its cell killing activity. It became evident that rotenone-inactivating substance(s) were produced in cells and stored in water-soluble form or liberated into the medium. The substance(s) were inactivated by heat treatment.

Animals↗
PubMedDate not supplied

An insect cell line discrimination method by RAPD-PCR.

RAPD-PCR with a tenmar single primer for discrimination of insect cell lines was devised. The base sequence of the primers used were TTCGAGCCAG, CGGCATCTAC, GAACGGACTC, and TGAGTGGGTG (GC contents were 60%). Genome DNA was extracted by modified Landry et al. (1993) method. The reaction mixture consisted of 10 microliters buffer, 8 microliters dNTP mixture (2.5 mM each), 4 microliters primer (50 microM), Taq DNA polymerase (2.5 units), 1 microliter template DNA; and the reaction was run at 94 degrees C for 2 min (denaturation), followed by 31 cycles of 94 degrees C for 1 min, 42 degrees C for 1 min (annealing), and 72 degrees C for 2 min (extension) and terminated with 72 degrees C for 7 min. By developing the reaction products with agarose gel electrophoresis, it became evident that DNA fragments were amplified with all the primers used. Among four primers, the second primer was selected as a suitable primer for distinguishing cell lines. With this method, cell lines derived from different species were clearly distinguished.

Animals↗