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Biomedical subjects

J Morand

Publications and source records attributed to J Morand.

6 recordsLinked to original sources
PubMed1991

Macronuclear transformation with specific DNA fragments controls the content of the new macronuclear genome in Paramecium tetraurelia.

A previously isolated mutant cell line called d48 contains a complete copy of the A surface antigen gene in the micronuclear genome, but the gene is not incorporated into the macronucleus. Previous experiments have shown that a cytoplasmic factor made in the wild-type macronucleus can rescue the mutant. Recently, S. Koizumi and S. Kobayashi (Mol. Cell. Biol. 9:4398-4401, 1989) observed that injection of a plasmid containing the A gene into the d48 macronucleus rescued the cell line after autogamy. It is shown here that an 8.8-kb EcoRI fragment containing only a portion of the A gene coding region is sufficient for the rescue of d48. The inability of other A gene fragments to rescue the mutant shows that this effect is dependent upon specific Paramecium DNA sequences. Rescue results in restoration of the wild-type DNA restriction pattern in the macronucleus. These results are consistent with a model in which the macronuclear A locus normally makes an additional gene product that is required for correct processing of the micronuclear copy of the A gene.

Animals↗
PubMed1985

Correlation between ketoprofen plasma levels and analgesic effect in acute lumbar pain and radicular pain.

Fourteen patients with acute lumbar pain and cervico-brachial radicular pain were treated with a single oral dose of 100 mg ketoprofen. The analgesic effect of the substance was investigated, using an analog pain scale, in correlation with drug plasma levels. Blood samples were taken at zero, one-half, one, two, three, four, five, six, eight, and ten hours after oral administration of ketoprofen. On an average, pain was at its lowest two hours after the plasma level of ketoprofen was at its highest. Within the ten hour observation period, the maximum reduction in pain increased as the maximum plasma level rose. Five patients with a maximum plasma level greater than 9 microliter/ml experienced pain reduction of at least 50%.

Acute Disease↗
PubMed1983

[Drug concentrations in blood, synovial fluid, synovial membrane, periarticular bone, muscle and adipose tissue in patients with rheumatoid polyarthritis receiving a single intramuscular injection of ketoprofen or acetylsalicylic acid (3 hours after the injection)].

The therapeutic activity of antiinflammatory agents in rheumatic joint disease is related to their presence at the target site of action, i.e. the joints. Tissue concentrations of such agents have been previously determined in patients with rheumatic disorders under long-term treatment with acemetacin and indomethacin (Köhler et al., 1981). The purpose of the present study was to determine concentrations of ketoprofen and acetylsalicylic acid in blood as well as synovial fluid, synovial membrane and periarticular bone and adipose tissue, three hours after administration of a single dose. Drug concentrations found in each of these tissues following a single intramuscular injection were sufficient to ensure therapeutic efficiency.

Adipose Tissue↗
PubMed1967

Floc sizing techniques.

In the study of mass transfer of substrate through floc particles, it is essential to have an estimate of floc size. This study presents floc size measurements made on pure culture bacterial floc and shows their applicability to mass transfer studies. A critical review of the biological literature on methods to size biological floc and determine wet floc density is also presented.

Bacteriological Techniques↗