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J Morizet

Publications and source records attributed to J Morizet.

24 records · Page 2Linked to original sources
PubMed1990

Enhanced in vitro cytotoxicity of 1,3-bis-(2-chloroethyl)-1-nitrosourea or buthionine sulfoximine combined with a reactive oxygen-generating enzyme immunotoxin.

The glutathione inhibitor drugs, 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) and buthionine sulfoximine (BSO), were tested in vitro in order to assess their cytotoxic effectiveness when combined with an enzyme immunotoxin (eIT) composed of a T-cell reactive monoclonal antibody (mAb) 097 coupled to the reactive oxygen-generating enzyme, glucose oxidase (GO) (EC 1.1.3.4). As targets of this eIT we used mature human T-cells or leukemia cells that expressed the 097 epitope. We found that treatment of the cells with subtoxic amounts of mixtures of both a drug and the 097 eIT markedly potentiated cytotoxicity compared to either drug or eIT alone.

Antineoplastic Combined Chemotherapy Protocols↗
PubMed1990

T cell depletion of human bone marrow using an oxidase-peroxidase enzyme immunotoxin.

We have shown in a previous paper that an antibody-enzyme immunotoxin (eIT) constructed by chemically coupling the 097 monoclonal antibody to glucose oxidase and to lactoperoxidase (097 eIT) effectively eliminated T cells of human peripheral blood origin and killed cultured human thymoma cells. Here we tested its effectiveness against T cells present in human bone marrow cell suspensions contaminated by large numbers of erythrocytes. The T cell-depleting capacity of the 097 eIT was assessed by means of four different assay methods, three of which gave concordant results and indicated an effective depletion comparable in efficiency to published work. For example, by limiting dilution analysis assay of IL-2-producing T cells we found approximately 3 logs of T cell depletion. The growth of bone marrow stem cells (CFU-GM, CFU-E and BFU-E) was not affected by treatment with the 097 conjugates.

Antibodies, Monoclonal↗
PubMed1989

An immunotoxin system intended for bone marrow purging composed of glucose oxidase and lactoperoxidase coupled to monoclonal antibody 097.

The effectiveness of an antibody-enzyme immunotoxin (eIT) was investigated on human T cells. This enzyme immunotoxin contained glucose oxidase (GO) and lactoperoxidase (LPO) chemically coupled to the pan-leukocyte-specific mouse monoclonal antibody (MoAb) 097 (097-GO and 097-LPO). Human peripheral blood mononuclear cells or tumor cells were suspended in a mixture of 097-GO and 097-LPO for 30 min, and then for 2 h with glucose and NaI. The effectiveness of this eIT system was indicated by the almost complete reduction of T cell viability, as estimated by a phytohemagglutinin induced proliferation assay (99.4% +/- 0.31 depletion, mean +/- SEM of 15 experiments). The specificity of the cytotoxicity reaction was indicated by the lack of cytotoxicity of control irrelevant MoAb conjugates to T cells (1.9% +/- 4.17 of T cell depletion, eight experiments). The growth of human bone marrow myeloid progenitors (CFU-GM) was not affected by the conjugates even by increasing 100-fold the optimal cytotoxic dose. T cells were susceptible to the conjugates in the presence of up to 90% of erythrocytes. This eIT system may thus represent a new alternative immunospecific procedure for allograft and/or autograft purging, and appears to effectively replace complement-mediated methods of T cell depletion.

Antibodies, Monoclonal↗
PubMed1981

[Crystallographic and molecular structure of trihexosyl-ceramide deposits characteristic of Fabry's disease (particularly the rheumatic form). Digital imaging processing].

The ceramide trihexoside inclusions, which characterize Fabry-Anderson disease, have been studied using electron microscopy and digital image processing. Results are given showing the various apparent periodicities of the inclusion lamellae and the orientation, perpendicular to the lamellae or oblique, of the ceramide molecular component.

Adipose Tissue↗
PubMed1980

[Fourier transformation study of corneal stroma].

The various patterns observed by generating Fourier transforms of the fixed normal corneal stroma are described. The patterns are analysed in terms of the dimensions of the stromal fibers, the dimensional and directional order of the fiber distribution and the order of the fiber-fiber functions by proteoglycans.

Collagen↗