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Biomedical subjects

J N Jaiswal

Publications and source records attributed to J N Jaiswal.

13 recordsLinked to original sources

Cytotoxicity of eugenol in sealer containing zinc-oxide.

In this study two zinc-oxide-based root canal sealers were compared for their tissue toxic response. The sealers tested were zinc-oxide eugenol and zinc-oxide glycerine. Fifteen albino rats were used for the study and were injected subcutaneously in the preset state. The tissue response was assessed by counting the polymorphonuclear cells at Day 1, Day 7 and Day 15 of the study period. The inflammatory response was graded according to the mean polymorphonuclear counts in the five rats for each period. The results showed that the toxicity was greater for the eugenol-containing sealer and increased during the three time intervals. For the non-eugenol sealer the response was milder and reached a peak by 7 days after which it decreased.

Animals

Antimicrobial efficacy of iodoformized zinc oxide-eugenol sealer on micro-organisms of root canal.

Iodoformized zinc oxide-eugenol was tested for its antibacterial effect against the aerobic and anaerobic bacteria obtained from the root canals of deciduous teeth; the bacteria were staph aureus, strep viridans, strep faecalis, bacteroides melaninogenicus and also mixed culture obtained from infected non-vital anterior teeth. The sensitivity was checked on BHI- agar plates employing well-method. Iodoformized zinc oxide eugenol paste was found to be effective for both the aerobic and anaerobic bacteria of the root canals of deciduous teeth with maximum sustaining period of 10 days; staphylococcus aureus was found to be the most susceptible, while Streptococcus faecalis the most resistant.

Bacteria

Pulp calcifications in primary teeth.

Information regarding the prevalence of pulp calcifications in primary teeth is lacking. The occurrence of pulp calcifications in 120 primary maxillary and mandibular extracted teeth was evaluated radiographically. Twenty-five percent of second molars presented evidence of pulp calcifications; approximately 3% of central incisors were calcified. No other categories of teeth displayed pulp involvement. The low occurrence of pulp calcifications in primary teeth support the view that the occurrence of pulp calcification increases with age.

Age Factors

Cross-sectional study to evaluate variations in attached gingiva and gingival sulcus in the three periods of dentition.

The dimensional differences of the attached gingiva and gingival sulcus in the primary, mixed and permanent dentition were studied clinically in normal gingivae of 382 children, aged 4-15 years. The depth of the gingival sulcus and the width of keratinized gingiva were also measured. Shallow gingival sulcus was observed in the primary dentition as compared to that in permanent dentition. However, in the mixed dentition deeper sulcus was observed around the newly erupted teeth. This was attributed to deeper penetration of the instrument, at the time of eruption. The widest zone of attached gingiva was observed in maxillary and mandibular incisors, decreasing gradually over the cuspids and first premolars (first primary molars), and again attached gingiva increased in second premolars (second primary molars) and first and second permanent molars. The width of attached gingiva in case of newly erupted permanent teeth (mixed dentition) was narrower in the corresponding primary teeth. This was due to an increase in the gingival sulcus depth. The attached gingiva increased in width with age. This was related to a concomitant reduction in sulcus depth.

Adolescent

The effect of metronidazole on the anaerobic microorganisms of the root canal--a clinical study.

The antibacterial effect of 0.25% metronidazole solution as root canal irrigant on anaerobic bacteria was studied in ten central incisors. Each tooth was treated at four appointments, and the presence of anaerobes in the root canal was studied on each occasion. No antibacterial intracanal dressings were used between the appointments. No anaerobes could be recovered from any tooth on the fifth appointment. In the control group, where normal saline was used as an irrigant, anaerobes could be recovered in four of the ten canals. These results suggest that 0.25% metronidazole solution is more effective than saline solution as a root canal irrigant.

Adolescent

Pulp calcifications in primary teeth.

One hundred and twenty primary maxillary and mandibular extracted teeth were collected to study the occurrence and nature of pulp calcifications. The teeth were serially sectioned, stained with hematoxylin and eosin, examined by light microscope. Pulp calcifications were observed in 31 (25.8%) teeth. Two types of pulp calcifications were noticed i.e., diffuse calcifications and free/attached type denticles. The occurrence of pulp calcification appears identical in all teeth except in the first primary molar which is statistically not significant (P greater than 0.05). The low prevalence of pulp calcifications in the primary teeth support the view that pulp calcification increases as the age advances.

Dental Pulp Calcification

Formocresol and glutaraldehyde pulpotomies in primary teeth.

The purpose of the present study was to compare clinically and radiologically the effects of formocresol and glutaraldehyde as a medicament in pulpotomized carious exposed vital human primary molars. In formocresol group 90% clinical and radiological success rate and in glutaraldehyde group 100% clinical and radiological success rate was observed. Thus it was concluded that glutaraldehyde is better fixative and less toxic agent than formocresol.

Aldehydes