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Biomedical subjects

J N Pal

Publications and source records attributed to J N Pal.

5 recordsLinked to original sources
PubMed1991

Isolation, purification and preliminary studies on fish eye lens glycoprotein.

Glycoprotein from the eye lens of fish, Mystus cavasius, was isolated by extraction with 1% Triton X-100 in saline. The crude extract which was found to be electrophoretically heterogeneous was fractionated on a DEAE-cellulose column. One of the fractions obtained in major amount was further resolved by column chromatography using Sephadex G-150 into two homogeneous fractions (GP-1 and GP-2]. GP-1 contained carbohydrates (11.2%) and protein (77.5%). The constituent sugars were D-glucose, D-mannose, D-galactosamine and N-acetyl neuraminic acid. The principal amino acids were aspartic acid, serine, glutamic acid, glycine and alanine. The proportions of these residues were determined.

Amino Acids↗
PubMed1989

Preliminary studies on excretory components of a virulent strain (F-2) of Pseudomonas solanacearum.

The excretory material (EM) was isolated from the culture medium of a virulent strain (F-2) of Pseudomonas solanacearum. Electrophoretic studies depicted the heterogeneous nature of EM and the presence of released lipopolysaccharide in it. Both the exopolysaccharide and the released LPS contained D-galactose as the major sugar constituent together with D-glucose, N-acetyl-D-galactosamine and N-acetyl-D-glucosamine. In addition, L-rhamnose was present as a constituent sugar of the released LPS.

Bacterial Proteins↗
PubMed1986

Aging studies on fingerprint residues using thin-layer and high performance liquid chromatography.

The chemical substances of perspiration found in latent fingerprint residue may hold the key for dating latent fingerprints. To study the chemical transformations of these components as a function of time and environment, fingerprint residues from four males and four females were analysed by thin-layer chromatography (TLC) and high performance liquid chromatography (HPLC). Three major and two or three minor components were detected by both methods and the variation in the quantity of the major components with time was observed.

Chromatography, High Pressure Liquid↗
PubMed1983

Immunochemical studies on Shigella dysenteriae type 10 bacterial polysaccharide.

On graded hydrolysis and Smith degradation, the O-somatic polysaccharide isolated from Shigella dysenteriae type 10 bacteria yielded four oligosaccharides which were characterized by methylation studies. Immunochemical studies using constituent monosaccharides and the oligosaccharides indicated that the sugar grouping leads to 3)-ManNAc-(1 leads to 3)-Rha-(1 leads to 4)-GlcNAc-(1 leads to was the immunodominant part in the polysaccharide molecule, and that 2-acetamido-2-deoxy-D-mannose contributed maximally to the immunological specificity of the macromolecule.

Carbohydrate Conformation↗