Diurnal variation in collections of hematopoietic cells?
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Circadian Rhythm↗
Biomedical subjects
J Nepomucka
Publications and source records attributed to J Nepomucka.
Blood count changes due to mononuclear cell apheresis.
Complete blood count and CFU-GM count of 41 healthy donors was determined immediately before and immediately after mononuclear cell (MNC) apheresis. Red blood cell count dropped from a mean of 5.2 to 4.9 x 10(12)/l, the difference being significant (p less than 0.001). Platelet count was reduced from a mean of 219 to 187 x 10(9)/l, the difference was once again significant (p less than 0.005). Polymorphonuclear cell count did not change significantly, while MNC count was reduced substantially (from mean 3.1 to 2.4 x 10(9)/l, p less than 0.001). The difference between pre- and postapheresis CFU-GM count (median 11.3 versus 8.8 x 10(3)/l respectively) was on the borderline of statistical significance (p = 0.05).
Adult↗
Intermittent-flow versus continuous-flow mononuclear cell apheresis.
An intermittent-flow (Haemonetics V-50) and a continuous-flow (Fenwall CS-3000) blood cell separator were compared regarding mononuclear cell (MNC) collection. 17 evaluable V-50 and 24 CS-3000 aphereses were performed on healthy donors. No significant difference was found in MNC yield (median 6.4 x 10(9) on V-50, 6.3 x 10(9) on CS-3000) or in CFU-GM yield (median 41 x 10(3) versus 43 x 10(3) respectively). Nevertheless, red blood cell as well as polymorphonuclear cell contamination was substantially greater on V-50 than on CS-3000, while platelet contamination was slightly lower on V-50 than on CS-3000. The continuous-flow apheresis system CS-3000 is concluded to be more suitable for MNC harvesting than the intermittent-flow system V-50.
Adult↗