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J Nishida

Publications and source records attributed to J Nishida.

At least 109 records · Page 6Linked to original sources
PubMed1987

[A case of extramedullary plasmacytoma].

The patient is 51-year-old male. A tumor arose on the upper eye lid at the age of 44 years and 6 years later, a tumor arose on the forearm. The tumor recurred 3 times on the forearm and were resected each times. When he was 51 years old, 3 tumors arose on the upper arm. Tumors were found in the cutis and subcutis of the jaw, back, forearm and thigh. A tumor was also found on the palatine tonsil. Bence Jones protein was negative in serum and urine. There were no particular findings in serum immunoelectrophoresis, bone X ray films, bone marrow aspiration and bone scintigram. The Borrmann 4-like pattern was revealed on the stomach by barium examination. By chemotherapy and radiotherapy, the tumors decreased markedly in size. Excisional biopsy showed that the tumor was composed of atypical plasma cells and gamma-chain and lambda-chain were immunohistologically detected.

Humans↗
PubMed1987

[Transforming gene in the preleukemic state].

The presence of a transforming gene in the DNA of bone marrow cells from patients with myelodysplastic syndrome (MDS) was studied using an in vivo selection assay in which NIH 3T3 cells transfected with human DNA were injected into nude mice in order to observe the growth of the tumor. The transforming gene was present in 12 out of 18 cases. The Alu sequence was demonstrated in the tumor grown after injection of transfected NIH 3T3 cells from 10 patients. Among these 10 Alu sequences, the human N-ras oncogene was present in 3 cases. Analysis of nucleotide sequences of the exons of human N-ras oncogenes cloned from the tumors revealed a single point mutation of the codon encoding the 13th amino acid of exon 1 from guanine to cytosine in all 3 cases of MDS. A one-year follow-up study of these MDS cases showed that in the patients positive for the transforming gene, the disease state progressed from PARA, PASA to RAEB or from RAEB to acute leukemia in 6 out of 7 cases, while in the 6 negative patients, no change was observed in their disease states. It was considered that the mutation of the N-ras gene at the 13th amino acid codon of exon 1 was fairly specific to MDS and that presence of the transforming gene may be used for predicting the progress of the disease.

Cell Transformation, Neoplastic↗
PubMed1985

Relationship between the concentration of myoglobin and parvalbumin in various types of muscle tissues from chickens.

The concentration of parvalbumin was determined in various types of chicken muscle by immunological analysis and was compared with that of myoglobin. Parvalbumin was present specifically in skeletal muscle and absent in cardiac and gizzard muscle; exceptionally, neither parvalbumin nor myoglobin was detected in white breast muscle. The wing and leg red muscles, which had larger amounts of myoglobin, contained smaller quantities of parvalbumin. In these muscles, the concentration of parvalbumin was inversely related to that of myoglobin (correlation coefficient = -0.69). Both myoglobin and parvalbumin were observed in the legs of 18-d-old embryos; the parvalbumin content exceeded that of myoglobin until the birds were 4 to 6 weeks old, but the relationship was reversed thereafter. Myoglobin in gizzard muscle was present in 18-d-old embryos and increased markedly at hatching; it was already present in cardiac muscle at an early embryonic stage, increasing gradually until 14 weeks after hatching.

Age Factors↗
PubMed1984

Isolation of HTLV derived from Japanese adult T-cell leukemia patients in human diploid fibroblast strain IMR90 and the biological characters of the infected cells.

HTLV was isolated in the human diploid fibroblast strain IMR90 directly from the leukemic cells of the Japanese ATL-patients. The infection was checked by the presence of virus-specific p24 and proviral DNA. The isolation was successful in three of four patients. The patient whose virus isolation was unsuccessful contained only a defective viral genome in his leukemic cells. The infected cells produced polykaryons when co-cultured with catS + L-cells. Human leukemia HL60 cells formed clusters adherent to the infected IMR90 cells but not to uninfected IMR90 cells. The former phenomenon was blocked by the ATLL patients' serum.

Cell Nucleus↗
PubMedDate not supplied

Eccentric simple bone cysts of the femoral neck in adults.

The purpose of this communication is to describe atypical simple bone cysts of the femoral neck seen in adult patients. Two patients, aged 56 and 49, having cystic lesions which did not conform to a typical simple bone cyst, are reported. Common features including eccentric location, and thick lining tissue and sclerotic margin are not those of typical simple bone cysts seen in children. Local mechanical characteristics might be related to the atypical presentations.

Bone Cysts↗
PubMedDate not supplied

A point mutation at codon 13 of the N-ras oncogene in myelodysplastic syndrome.

Patients with a myelodysplastic syndrome (MDS) which has a risk of leukaemic change exhibit a variable clinical course. It has been suggested that the development of leukaemia in patients with MDS may be related to chromosomal abnormalities or genetic alterations: somatic mutation of the N-ras gene is now considered to be a critical step in the genetic basis of human leukaemogenesis. Here we report that DNAs of bone-marrow cells from three out of eight patients with MDS contained an activated N-ras oncogene, as detected by an in vivo selection assay in nude mice with transfected NIH 3T3 cells. Molecular analysis revealed the same single nucleotide substitution at codon 13 in all three transforming N-ras genes. Each of the three patients showed a progression of the disease and a resulting leukaemic change within the following year. Our observation of the mutation at codon 13 in leukaemic cell DNAs from all three cases suggests that activation of the N-ras gene is important in the development of leukaemia in some MDS cases.

Amino Acid Sequence↗
PubMedDate not supplied

Familial occurrence of telangiectatic osteosarcoma: cousin cases.

Familial occurrence of osteosarcoma is rare. We report cousin cases, an 11-year-old girl and an 8-year-old boy with telangiectatic osteosarcoma. The tumors occurred in the metaphysis of the distal tibia and the lamina of thoracic vertebra. The local behaviors appeared aggressive, but the clinical courses were relatively indolent in both cases. The histologic features were similar, showing anaplastic tumor cells producing osteoid and proliferation of blood cavities. Clustering of malignancies within a family suggests the presence of a genetic factor.

Bone Neoplasms↗