In the extension of our previous work on alkali burns of the rabbit cornea we have studied acid burns of the rabbit cornea using a ring-shaped chromoatographic paper soaked with solutions of different kinds of acids. The ensuing changes were studied with the silver methods of McGovern and Jabonero.

Acids↗

PubMed1976

Indentification and radioimmunologic estimation of sexual steroid hormones in aqueous humor and vitreous of rabbit eye.

The occurrence of testosterone, estrone, and estradiol-17 alpha in aqueous humor and vitreous body of the rabbit eye was demonstrated. The identity of the steroid hormones was confirmed by their chromatographic properties, microreactions, and radiochemically by crystallization to constant specific activity of their labeled acetates. The levels of testosterone and estrogens in rabbit male and female plasma, aqueous, and vitreous were determined by radioimmunologic assay. The concentrations of the steroid hormones in plasma are higher than in aqueous and vitreous. However, testosterone in aqueous circulates in free form and lacks the binding to sexual hormone-binding globulin as in plasma.

Animals↗

PubMed1976

Ring-shaped alkali burns of the rabbit cornea. III. Late phase of the stroma reaction.

The present paper deals with late changes of the corneal stroma after alkali burns from the 5th day after the injury up to the 58th day. As has been pointed out in a previous paper, between the 3rd and 5th day the process of healing within the corneal stroma changes and at the end a dense scar ensues.

Animals↗

PubMed1976

Ring-shaped alkali burns of the rabbit cornea. II. Early changes of the corneal stroma.

Corneas of 32 rabbits were coagulated in a circular zone of 1 mm width and 10 mm diameter. The results of the experiment showed sharply limited injury throughout the entire depth of the cornea without lateral diffusion. The reactions of the corneal stroma during the first three days are described and discussed.

Animals↗

PubMed1976

Changes of activity of alkaline and acid phosphatase in the rabbit eye in the early phase of alkaline and acid injury.

Rabbit corneas were burned either with 1.0 N sodium hydroxide or 1.0 N hydrochloric acid. Enzyme activities of alkaline and acid phosphatases were examined spectrophotometrically in the homogenates of cornea, iris, aqueous humor and vitreous body. On the 3rd day after alkaline as well as acid burn, a significant decrease of both enzyme activities was produced as compared with untreated animals. A more pronounced change was found in the case of alkaline injuries. With both kinds of caustic agents the decrease of acid phosphatase activity was more striking than that of the alkaline phosphatase. Advantages and shortcomings of biochemical and histochemical enzymatic determinations in experimental ocular inflammations are briefly discussed.

Acid Phosphatase↗

PubMed1976