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Biomedical subjects

J Oleszczuk

Publications and source records attributed to J Oleszczuk.

At least 19 recordsLinked to original sources
PubMed2005

Apoptosis of HeLa cell lines incubated with retinol.

PURPOSE: The aim of the study was to evaluate a soluble form of APO-1/Fas antigen in supernatants from HeLa cell line culture after 24 and 72 h of incubation with selected retinoic acid concentrations. MATERIALS AND METHODS: HPV18-positive cell lines were cultivated with All-trans-retinoic acid (ATRA) at concentrations of 1 x 10(-6) and 1 x 10(-9) M. The cultures were incubated for 24 and 72 h. A control culture with 3 microl of DMSO was incubated under identical conditions. The concentrations of soluble APO-1/Fas antigen in cell culture supernatants were estimated using an ELISA method. RESULTS: The culture with 72-h incubation with retinoic acid proved to be toxic to cells and was excluded from the analysis. The results obtained showed a significantly lower concentrations of soluble APO-1/Fas antigen in supernatants from cell lines incubated with retinol for 24 h than in the controls. CONCLUSIONS: The higher concentrations of soluble APO-1/Fas antigen in supernatants from the HeLa cell line without retinol may constitute a protective mechanism of the cells infected with the virus before undergoing Fas/FasL-dependent apoptosis. Lower concentrations of sAPO-1/Fas antigen in the supernatant from HeLa cell culture incubated with retinol may suggest that mechanisms protecting infected cells against Fas/FasL-mediated apoptosis become defective under the influence of retinol. Our studies confirm that Vitamin A and its analogues inhibit the proliferation of cells associated with HPV infection and suggest promising effects of retinoid therapy in inhibiting the progression of early cervical lesions to cancer.

Antineoplastic Agents↗
PubMed2004

CD1c(+) immature myeloid dendritic cells are predominant in cord blood of healthy neonates.

It has been suggested lately that some types of antigen presenting cells-myeloid dendritic (DC-1) cells can differentiate the immune response towards Th1 type immunity, whereas lymphoid cells (DC-2) can stimulate Th2 type immunity. It has been observed that neonates are deficient in Th1 response. The purpose of our study was to estimate the proportions of immature myeloid (CD1c(+)) and lymphoid (BDCA-2(+), BDCA-4(+)) dendritic cells and the CD1c(+):BDCA-2(+) cell ratio in cord blood of healthy neonates in comparison with dendritic cells of healthy adults. Thirty healthy neonates born from normal pregnancies and 30 healthy adults were included in the study. The dendritic cells were isolated from cord and peripheral blood, stained with anti-CD1c, anti-BDCA-2, anti-BDCA-4, anti-CD123 and anti-CD19 monoclonal antibodies and estimated using flow cytometry. The percentage of CD1c(+) dendritic cells in cord blood of healthy newborns did not differ significantly when compared to those in peripheral blood of healthy adults. The percentages of cord blood BDCA-2(+) and BDCA-4(+) dendritic cells of neonates were significantly lower when compared to lymphoid dendritic cells in peripheral blood of adults. The CD1c(+):BDCA-2(+) ratio was significantly higher in cord blood of neonates in comparison with CD1c(+):BDCA-2(+) ratio in adult's blood. Myeloid and lymphoid dendritic cells may be involved in the immune regulation during fetal development. Immature myeloid dendritic cells are predominant in cord blood of healthy neonates. Immature lymphoid dendritic cells are not the major population of dendritic cells in cord blood.

Adult↗
PubMed2004

Evaluation of placental angiotensin type 1 receptors in women with hypertension during pregnancy.

OBJECTIVE: The aim of this study was to examine and compare the localization and immunoreactivity of the angiotensin type-1 receptor (AT1R) in human placental tissue in patients with pregnancy complicated by pre-eclampsia with and without intrauterine growth restriction (IUGR). METHODS: Immunohistochemistry was used to examine the localization of the AT1R in human placental tissue. Semiquantitative immunohistochemical H-score values for each placental cell type were calculated. RESULTS: Elevated H-score index values for decidual cells, syncytiotrophoblast and cytotrophoblast in pre-eclamptic patients were found to be higher in comparison with values in the control group. Decreased H-score index values for syncytiotrophoblast and cytotrophoblast were found in patients with pregnancy complicated by IUGR in the course of pre-eclampsia. CONCLUSIONS: The results obtained in this study point out the significant role of the AT1R and suggest that normal AT1R activity is of fundamental significance for the normal course of pregnancy and proper fetal growth.

Case-Control Studies↗
PubMed2003

Blood myeloid and lymphoid dendritic cells are stable during the menstrual cycle but deficient during mid-gestation.

The aim of this study was to estimate the populations of peripheral blood myeloid and lymphoid dendritic cells (CD1c(+), BDCA-2(+), BDCA-4(+)) and the CD1c(+):BDCA-2(+) ratio in phases of the ovarian cycle and in normal pregnant patients. 18 non-pregnant women and 17 normal pregnant women were included. Dendritic cells were isolated from peripheral blood, stained with monoclonal antibodies (mAbs) against blood dendritic cell antigens (anti-BDCA-1, BDCA-2, BDCA-4) and estimated using flow cytometry. CD1c(+), BDCA-2(+) and BDCA-4(+) dendritic cells were present in the follicular and luteal phases of the ovarian cycle and in all trimesters of normal pregnancy. The percentages of CD1c(+) dendritic cells did not differ between the follicular and luteal phases of the ovarian cycle. The percentage of BDCA-2(+) dendritic cells was lower in the luteal phase of the ovarian cycle compared with the follicular phase, but the differences were not statistically significant. The CD1c(+):BDCA-2(+) cell ratio was significantly lower in the luteal phase compared with the follicular phase of the ovarian cycle. The numbers of dendritic cells were significantly lower in the second trimester when compared with the first and third trimesters of normal pregnancy. Furthermore, in the second trimester, the CD1c(+):BDCA-2(+) ratio was higher than in the other trimesters of normal pregnancy. All populations of dendritic cells and the CD1c(+):BDCA-2(+) ratio did not differ in the first and third trimesters of physiological pregnancy. Our results suggest that myeloid and lymphoid dendritic cells are not affected by steroid hormones during the menstrual cycle. The deficiency of peripheral blood dendritic cells observed during the second trimester of normal pregnancy can be associated with their migration to the uterus during the second physiological invasion by cytotrophoblast.

Adult↗
PubMed2003

Myeloid and lymphoid dendritic cells in normal pregnancy and pre-eclampsia.

The aim of our study was to estimate the populations of peripheral blood myeloid and lymphoid dendritic cells (CD1c+, BDCA-2+) and the CD1c+ : BDCA-2+ ratio in normal pregnant women and in patients with pre-eclampsia. Fifteen women in the first, second and third trimesters of normal pregnancy, and 25 patients with pre-eclampsia were included in the study. The dendritic cells were isolated from peripheral blood, stained with monoclonal antibodies against blood dendritic cell antigens (anti-CD1c, anti-BDCA-2) and estimated using the flow cytometric method. CD1c+ and BDCA-2+ dendritic cells were present in women during all trimesters of physiological pregnancy and in pre-eclamptic patients. It was observed that the numbers of dendritic cells were significantly lower in the second trimester when compared with the first and third trimesters of normal pregnancy. Furthermore, in the second trimester, CD1c+ : BDCA-2+ ratio was higher than in the other trimesters of physiological pregnancy. All populations of dendritic cells and CD1c+ : BDCA-2+ ratio did not differ in the first and third trimesters of normal pregnancy. The percentage of BDCA-2+ dendritic cells was significantly lower in pre-eclampsia in comparison with healthy women in the third trimester of physiological pregnancy, while CD1c+ : BDCA-2+ ratio was significantly higher in pre-eclamptic patients when compared with control groups. We concluded that dendritic cells may be involved in the immune regulation during physiological pregnancy. CD1c+ and BDCA-2+ cells can influence the Th2 phenomenon which is observed during physiological pregnancy. Furthermore, it seems possible that lower BDCA-2+ cells percentage and higher CD1c+ : BDCA-2+ ratio can be associated with increased Th1-type immunity in patients with pre-eclampsia.

Antigens, CD1↗
PubMed2002

Intrapartum cardiotocography and fetal pulse oximetry in assessing fetal hypoxia.

OBJECTIVES: A retrospective analysis of short-term variability (STV), a cardiotocography (CTG) parameter, in relation to fetal blood saturation values (FSpO(2)) obtained by fetal pulse oximetry. METHODS: The study included 26 healthy pregnant women monitored continuously during delivery with both cardiotocography and fetal pulse oximetry. RESULTS: Lower FSpO(2) values were observed in the group showing STV levels 6.0 ms (34.4+/-2.9% vs. 43+/-7.2%; P<0.001). A positive correlation was found between STV levels <or=6.0 ms and mean FSpO(2) values in the 2nd stage of labor. CONCLUSIONS: 1. A significant relationship was observed between short-term variability in the cardiotocographic records and fetal blood saturation levels in the 2nd stage of labor. 2. Fetal pulse oximetry can be valuable in assessing fetal wellbeing, especially when CTG records are abnormal.

Cardiotocography↗
PubMed2001

Pre-eclampsia affects the immunophenotype of neonates.

OBJECTIVE: We tried to estimate whether immunological changes are present in neonates born to mothers who had been suffering from pre-eclampsia. STUDY DESIGN: Eighteen neonates born to mothers with severe pre-eclampsia (between 35 and 40 weeks of gestation) and 20 full-term healthy newborns (between 38 and 40 weeks of gestation) were included in the study. The lymphocytes were isolated from umbilical cord blood. The specific lymphocyte antigens were determined using direct staining with monoclonal antibodies and analysed by flow-cytometry. RESULTS: We observed that neonates born to pre-eclamptic mothers had decreased percentage of T CD 3(+), CD 4(+) and T CD 8(+)28(+) (cytotoxic) lymphocytes and increased percentage CD 3(-)16/56(+) cells and CD 8(+)28(-) (suppressor) lymphocytes in comparison with newborns of healthy women. Furthermore, we found decreased CD 4: CD 8 lymphocyte ratio in the study group in comparison with the control group. We also observed that the percentage of CD 19(+)5(+), CD 4(+)8(+), CD 19(+)40(+) and CD 3(+)40L(+) lymphocytes did not differ in both studied groups. The percentage of CD 4(+)45RO(+), CD 8(+)45RO(+) memory cells was higher in neonates born to pre-eclamptic mothers when compared to controls. Moreover, the expression of CD 25 molecule was higher on T CD 8(+) and B CD 19(+) lymphocytes of neonates of pre-eclamptic mothers. CONCLUSION: The alterations in the immunological parameters of neonates born to pre-eclamptic mothers can be associated with the maternal disease.

B-Lymphocyte Subsets↗
PubMed2001

Nitric oxide for treatment of threatened preterm labor.

OBJECTIVE: The aim of our study was the assessment of effectiveness of nitroglycerin as a donor of nitric oxide, administered in the form of transdermal therapeutic system, applied in the treatment of threatening preterm labor. PATIENTS AND METHODS: The study was carried out on 30 pregnant patients with the symptoms of threatening preterm labor between 27th and 34th week of gestation. The patients were given nitroglycerin in the form of transdermal system releasing 5 mg of nitroglycerin in 24 h. RESULTS: In our study the decrease in contractility and relaxation of uterus was observed in all obstetric patients. No changes in the fetal pulse rate and cardiotocographic tracing in the course of treatment and after completing treatment were observed. CONCLUSION: Nitroglycerin in the form of transdermal therapeutic system releasing nitric oxide may be an effective and safe tocolytic drug, however, further investigation needs to be performed.

Administration, Cutaneous↗
PubMed2001

The expression and concentrations of Fas/APO-1 (CD95) antigen in patients with severe pre-eclampsia.

PROBLEM: Apoptosis has been proposed as a mechanism for maintaining homeostasis in the immune system. Activated lymphocytes are removed by a Fas/FasL-mediated programmed cell death process called activation induced cell death (AICD). The aim of the study was to investigate surface Fas antigen (APO-1, CD95) expression on T lymphocytes and NK cells and also soluble Fas antigen concentrations in pre-eclamptic patients and healthy pregnant women. MATERIALS AND METHODS: Sixteen pre-eclamptic patients and 18 healthy pregnant women were studied. Peripheral blood lymphocytes were isolated, labeled by direct staining with anti-CD95 monoclonal antibodies and analyzed using the flow cytometric method. Furthermore, the concentrations of soluble CD95 molecule in serum of patients with severe pre-eclampsia and women with uncomplicated pregnancy were measured using ELISA method. RESULTS: We found that Fas antigen expression and fluorescence intensity on T CD8+ lymphocytes were higher in patients with severe pre-eclampsia in comparison with healthy pregnant women (P<0.05). Furthermore, the concentrations of soluble CD95 molecule were higher in the group of pre-eclamptic patients when compared to controls (P<0.001). CONCLUSIONS: These findings suggest that T CD8+ lymphocytes in patients with severe pre-eclampsia can be activated. Moreover, higher concentrations of soluble CD95 antigen can suggest altered possibilities to undergo Fas/FasL-mediated activation induced cell death process of lymphocytes in severe pre-eclampsia.

Adult↗
PubMed2001

The concentrations of osteocalcin and degradation products of type I collagen in pregnant women with pre-eclampsia.

OBJECTIVES: To investigate the concentrations of osteocalcin and collagen type I C-terminal telopeptides in pregnant women with pre-eclampsia. STUDY DESIGN: 26 patients with severe pre-eclampsia and 24 healthy pregnant women were included in the study. Serum concentrations of osteocalcin and C-telopeptides--degradation products of type I collagen were determined using the ELISA method. Statistical analysis was performed using Mann-Whitney U-test. RESULTS: In pre-eclamptic patients, the concentrations of osteocalcin and degradation products of collagen type I were significantly higher (P<0.005) when compared to healthy pregnant women. CONCLUSION: These results could suggest that there are alterations in bone metabolism in pregnant women with pre-eclampsia.

Adult↗
PubMed2001

Pregnancy in women with epilepsy.

The aim of this study was to analyze the outcome of pregnancy and delivery in epileptic women. A retrospective review of the 41 pregnant women with epilepsy who delivered in the Department of Obstetrics and Perinatology of the University School of Medicine in Lublin over 7 years (1993-1999) was carried out. Women with epilepsy had more pregnancy complications including premature labor, anemia, hypertension, vaginal bleeding, urinary tract infection, nausea and vomiting. An increased risk of congenital malformations and intrauterine fetal growth retardation was observed. Women with epilepsy require more extensive pregnancy planning including neurologic and preconceptional care.

Adult↗
PubMed2001

[Amniocentesis and mother's bladder overfilling in operative treatment of advanced cervical incompetence].

We describe two cases treated for cervical incompetence with prolapsed fetal membranes with emergency cerclage. Two methods of membranes reducing were used: transabdominal amniocentesis and bladder overfilling. We recommend these procedures in patients with advanced cervical incompetence when the membrane reduction by Trendelenburg position or pharmacological tocolysis were ineffective.

Administration, Intravesical↗
PubMed2001

[The relationship between the levels of proinflammatory cytokines, and AFI value in pregnancies complicated by premature rupture of membranes].

OBJECTIVE: The aim of the study was to test the relationship between maternal serum proinflammatory cytokines level and AFI (amniotic fluid index) in pregnancies complicated by premature rupture of membranes (PROM). MATERIALS AND METHODS: The maternal serum levels of IL-6, IL-1 beta and TNF-alfa were assessed in patients with PROM between 24-34 weeks of gestation (n = 45) by means of commercially available ELISA assays. The patients were divided in two groups according to AFI values: < 50 (n = 25) mm and < or = 50 mm (n = 20). Cytokine concentrations were compared between groups. RESULTS: The median concentrations of proinflammatory cytokines in maternal serum were: IL-6--5.74 pg/ml (range 3.24-3111 pg/ml), IL-1 beta--0.76 pg/ml (range 0.001-3.16 pg/ml), TNF-alfa--1332.46 pg/ml (range 2.13-1969.68 pg/ml). Compared to patients with AFI values > or = 50 mm, the group of patients with AFI < 50 mm had significantly higher concentration of IL-6 (6.61 pg/ml vs. 4.66 pg/ml; p = 0.002). No significant differences in IL-1 beta and TNF-alfa levels have been found between groups. The significant correlation have been observed between maternal serum level of IL-6 and AFI values (R = -0.47, p = 0.003), but not of IL-1 beta and TNF-alfa. CONCLUSION: The assessment of AFI values in pregnancies complicated by premature rupture of membranes seems to be the valuable method of early diagnosis in cases of intrauterine infection.

Adult↗
PubMed2001

[The usefulness of the intrapartum fetal pulse oximetry in anticipating the neonatal outcome].

OBJECTIVE: The aim of the study was to evaluate the usefulness of the intrapartum fetal pulse oximetry in anticipating the neonatal outcome. MATERIALS AND METHODS: The saturation of the fetal blood (SpO2) was measured during labor with non-invasive pulse oximeter designed for fetal application. The average, minimum and maximum SpO2 were evaluated separately for the first and the second stage of labor. The average SpO2 of the fetus was compared to neonatal condition assessed by umbilical vein pH, pO2 and pCO2 and according to Apgar score. RESULTS: Twenty patients have been monitored with fetal pulse oximetry. All those patients had normal vaginal delivery. During the first stage of labor, the average fetal SpO2 was 51.94 +/- 8.03%, the minimum SpO2 was 38.35 +/- 9.15%, and the maximum SpO2 was 63.35 +/- 7.75%; in the second stage of labor average fetal SpO2 was 43.82 +/- 7.16%, minimum SpO2 was 34.35 +/- 7.79% and the maximum SpO2 was 50.94 +/- 8.37%. A significant decrease in fetal average and maximum SpO2 occurred from stage I to stage II of labor (average SpO2: 51.94 +/- 8.03% vs. 43.82 +/- 7.16%, p = 0.0002; maximum SpO2: 63.35 +/- 7.75% vs. 50.94 +/- 8.37%, p < 0.00001). The significant correlation between the average SpO2 during the first stage of labor and umbilical vein pH (R = 0.60, p = 0.02) and pO2 (R = 0.54, p = 0.04) was found. No relationship between fetal SpO2 in the first and second stage of labor and Apgar score was observed. CONCLUSIONS: 1. The second stage of labor results in significant decrease in fetal SpO2. 2. The fetal SpO2 > 30% in the first and second stage of labor is related to good neonatal outcome. 3. The fetal SpO2 assessment in first stage of labor seems to be important in newborn's acidosis and hypoxemia predicting.

Acidosis↗
PubMed2001

[Evaluation of the platelet angiotensin receptor in pregnancy complicated by preeclampsia].

UNLABELLED: Preeclampsia is a major complication during human pregnancy. It results from a breakdown in the balance between the vasoconstrictor and vasodilator substances. Angiotensin II is a potent vasoconstrictor, which participates in the regulation of blood pressure and may be involved in the control of vascular tone. OBJECTIVE: The purpose of this study was to determine the platelet angiotensin II receptor number and angiotensin II level in pregnancies complicated by preeclampsia. Preeclampsia was defined according to American College of Obstetricians and Gynecologists (ACOG) classification. PATIENTS AND METHODS: Ligand binding techniques were used for the examination of platelet angiotensin II binding sites in the third trimester pregnant women. The study was carried out in 13 patients with singleton pregnancy complicated by preeclampsia. A control group consisted of 17 healthy normotensive patients with singleton uncomplicated pregnancy and normal laboratory tests. All studied patients were nonsmokers. RESULTS AND CONCLUSIONS: There were no statistically significant differences in patient profiles between groups including gravidity, parity, maternal age, gestational age and height. Maternal weight, BMI and systolic, diastolic blood pressure and mean arterial blood pressure (MAP) were higher in the study group in comparison with the control group. Our study revealed elevated platelet angiotensin II receptor number and decreased maternal angiotensin II level in singleton pregnancies complicated by preeclampsia. There were no correlations between platelet angiotensin II receptor number and plasma angiotensin II level in the studied subjects. Our results are in accord with other published data and point out to the significant role of renin-angiotensin-aldosterone system and angiotensin II receptors in the pathogenesis of preeclampsia.

Adult↗