Mechanism of hemolysis of G-6-PD deficient red cells: changes in membrane lipids and polypeptides.
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Biomedical subjects
Publications and source records attributed to J P Bapat.
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A new variant of hereditary hemolytic anemia in a family due to high adenosine triphosphate (ATP) is reported. The increase in ATP levels varied from 83 to 105% in the family members. Low 2,3-diphosphoglycerate levels and low 2,3-diphosphoglyceromutase activity were observed in three family members, with normal glucose-6-phosphate dehydrogenase and pyruvate kinase activity.
Examination of the red cell enzyme profile in a case of congenital methemoglobinemia has shown associated deficiencies of glutathione reductase (GR) and glutathione peroxidase (GSHPx) in addition to NADH-methemoglobin reductase deficiency. Contrary to expectations, GR and GSHPx deficiencies do not seem to have contributed to the methemoglobinemia in this case. The lack of symptoms in spite of a high methemoglobin (Hi) level (35%) appears to be due to the restriction of Hi to a small percentage of red cells.
HBsAg subtype distribution of 105 positive samples showed 91.4% as ay type. The ay was predominant in different disease conditions, healthy carriers and posttransfusion hepatitis. The prevalence of ay in the Bombay sample is in contrast to the predominance of ad reported from North India. These findings may suggest multifocal infection in different regions of India.
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A laboratory method for the indirect detection of hepatitis-B antigen (HB8Ag) based on the presence of 'anomalous' LDH isoenzyme by disc electrophoresis has been developed. The method is specific and highly sensitive when compared with existing methods.
Four groups of Lohanas, belonging to the Gujarati, Sindhi and Punjabi were studied for various genetic markers. Lohanas have higher B than A and low Rh(D) negative (1.65-4.64%). The Hp1 gene ranges from 0.1557 to 0.2639; Gm1 is lower (0.34-0.55) than in other populations in Southern India. G-6-PD deficiency was prevalent in 3-8%. All the four groups have a high incidence of the thalassaemia trait and possess Hb-D. Hb, J, and L were also observed in two groups. Data was analysed for intergroup differences.
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