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J P Casey

Publications and source records attributed to J P Casey.

8 recordsLinked to original sources
PubMed1993

Evidence for alpha and Mu class glutathione S-transferases in a number of fungal species.

1. The glutathione S-transferases of the following fungal species have been affinity purified on glutathione-Sepharose; Yarrowia lipolytica (NCYC825), Sporotrichum thermophile (ATCC16479), Talaromyces emersonii (ATCC26914) and Issatchenkia orientalis (CBS5147 and an isolate provided by Prof. Kumagai, Kyoto University). 2. SDS-PAGE analysis of affinity purified fungal extracts, followed by Western blots with polyclonal antisera to rat GSTs, was performed and indicated the presence of Alpha and Mu class glutathione S-transferases in the fungi immunologically and electrophoretically similar to the rat enzymes. 3. Each extract gave a characteristic affinity-gradient elution pattern on glutathione-agarose.

Animals↗
PubMed1988

Chemistry set chemicals: an evaluation of their toxic potential.

KM, a 14-year-old female packed 3 empty Sudafed SA capsules with 3 parts sodium chloride and 1 part cobalt chloride obtained from her brother's chemistry set. Following this, she ingested all 3 capsules. While the child had an uneventful hospital course, cobalt serum levels drawn 12 and 22 hr post-ingestion were measured at 7.8 micrograms/dL and 0.7 micrograms/dL, respectively. Due to the limited amount of data on the toxicity of cobalt chloride and the nature of the exposure, the case prompted a review of the potential toxicity of chemistry set chemicals available to consumers. The contents of 3 chemistry sets were evaluated. Each contained either 28, 14, or 9 chemicals. A total of 38 different chemicals were weighted and evaluated to determine whether the amount contained represented a potentially toxic or lethal dose to a 2-year-old, 12 kg child. Fifty-three percent of the chemicals evaluated contained quantities sufficient to result in toxicity. Thirteen percent of chemicals contained potentially lethal quantities, while 18% were considered non-toxic. Sixteen percent of the chemicals could not be evaluated due to lack of data in the literature. Only one chemistry set utilized child resistant closures. However, 65% of all potentially toxic chemicals and 100% of potentially lethal chemicals had appropriate first aid instructions.

Adolescent↗
PubMed1979

The effect of methylprednisolone on hepatic oxygen supply and plasma lactate and glucose in endotoxemia.

This study was designed to determine the effect of methylprednisolone on the profile of hepatic oxygen supply and selected blood parameters in fasted, male rats administered an LD85 dosage of E coli endotoxin intraperitoneally. Mortality rates within 24 hours were 85% in rats receiving endotoxin only, 9% in rats receiving a 30 mg/kg dosage of methylprednisolone intraarterially one hour subsequent to endotoxin insult, and 0% in methylprednisolone controls. Beginning with the fourth hour, untreated endotoxin rats had significantly higher heart rates and lower plasma glucose; by the sixth or eighth hour there was significantly greater hypocapnia, lower blood pH, and higher plasma lactate levels in comparison to endotoxic rats receiving methylprednisolone. In addition, mean hepatic pO2 between the sixth and seventh hours was 2.6 mm Hg in endotoxic rats, 10.6 mm Hg in endotoxic methylprednisolone rats, and 17.7 mm Hg in methylprednisolone controls. Methylprednisolone controls showed a steady increase of plasma glucose levels through eight hours but were otherwise stable. Maintenance of hepatic circulation is cited as the probable basis for differences of morbidity and mortality between treated and glucocorticoid-treated endotoxic rats.

Animals↗