Biomedical subjects

J P Goldring

Publications and source records attributed to J P Goldring.

8 recordsLinked to original sources

PubMed1999

Glucocorticoids, antioxidants and staurosporine modulate the adherence between monocytes and malaria infected erythrocytes.

OBJECTIVE AND DESIGN: Adherence interactions involving monocytes are important for cell-cell interaction as an integral component of immune function. The adherence of malaria parasitised red cells to monocytes was determined after monocytes were treated with dexamethasone, cortisol, ambroxol, danazol, probucol and staurosporine. MATERIALS: Human peripheral blood monocytes isolated by density gradient centrifugation and adherence to glass cover slips. METHODS: The adherence of malaria parasitised red cells to monocytes was determined after the monocytes were incubated for 24 h in the presence of each of the 6 drugs. RESULTS: The two glucocorticoids and staurosporine reduced the adherence of malaria infected erythrocytes to monocytes in a dose dependent manner at concentrations from 10(-10) M and above and ambroxol, danazol, and probucol at 10(-5) M. Staurosporine was the most effective of the drugs studied, completely abolishing adherence at 10(-6) M. CONCLUSION: The adherence of malaria infected erythrocytes to monocytes is reduced in response to glucocorticoids (dexamethasone and cortisol), anti-oxidants (probucol and ambroxol), danazol and staurosporine.

Anti-Inflammatory Agents↗

PubMed1999

Antimalarial drugs modulate the expression of monocyte receptors.

The cytoadherence of four Plasmodium falciparum malaria isolates (FCR-3, RSA-14, 15 and 17) to monocytes was used as a measure of the expression of monocyte receptors after the monocytes had been exposed to seven antimalarial drugs. Quinine, chloroquine, primaquine, pyrimethamine, artemesinin, mefloquine and proguanil all down-regulated the expression of monocyte receptors by 40% or greater at the therapeutic concentrations of each drug. Each malaria isolate had a unique adherence profile for drug induced changes in monocytes. Each drug appeared to alter the expression of more than one monocyte receptor. The most effective drugs were quinine, pyrimethamine and palludrin and the least effective were artemether and mefloquine. The results suggest a previously undetected immunomodulatory action of antimalarial drugs.

Animals↗

PubMed1997

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Bibliometrics↗

PubMed1996

Solubilization of protein-dye complexes on nitrocellulose to quantify proteins spectrophotometrically.

Proteins absorbed directly onto nitrocellulose membranes were stained with amido black, ponceau S, colloidal silver, or Coomassie blue and solubilized in dimethyl sulfoxide and the absorbance was measured spectrophotometrically. The optimal wavelength of each dye/protein/nitrocellulose solution was found to be at 625, 529, 420, and 600 nm, respectively. A linear relationship was found between the protein concentration and absorbance at the appropriate wavelength for all the stains with individual purified proteins or protein mixtures. Protein (0.2-0.8 microgram) can be determined with the colloidal silver and 2-30 micrograms with the other stains. Coomassie blue produced variable background staining of the nitrocellulose and is therefore not recommended. Proteins transferred electrophoretically to nitrocellulose from a sodium dodecyl sulfate-polyacrylamide gel were also stained with the above dyes and solubilized in dimethyl sulfoxide. Amido black was the most sensitive stain, detecting proteins in the range of 1-10 micrograms. Components of the gel interfered with silver staining.

Adsorption↗

PubMed1994

Insect acetyl-CoA carboxylase: enzyme activity during adult development and after feeding in the tsetse fly, Glossina morsitans.

Acetyl-CoA carboxylase (EC 6.1.4.2) activity in the adult tsetse fly (Glossina morsitans) increased 2-3 days after pupation to reach a plateau of between 0.4 and 0.6 mumol/min/mg after 7 days, and between 0.6 and 0.8 mumol/min/mg after 6 days in the abdomens of male and female flies, respectively. The enzyme showed a 50-70% increase in specific activity within 20 hr after a blood meal in previously starved flies. Lipogenesis and acetyl-CoA carboxylase activity were detected in the thorax, the abdominal cuticle and, in greatest quantity, in the fat body.

Abdomen↗

PubMed1993

Insect acetyl-CoA carboxylase: activity during the larval, pupal and adult stages of insect development.

1. The activity of the lipogenic enzyme, acetyl-CoA carboxylase, was investigated in four insect species; Bombyx mori (Lepidoptera), Tenebrio molitor (Coleoptera), Glossina morsitans and Sarcophaga nodosa (Diptera). 2. Acetyl-CoA carboxylase activity in larval, pupal and adult forms was compared with the saponifiable lipid mass at each stage of the life-cycle, and found to follow similar patterns except for Tenebrio molitor. 3. The results are examined in relation to known metabolic requirements for each insect.

Acetyl-CoA Carboxylase↗

PubMed1989

Cloned T cells provide help for malaria-specific polyclonal antibody responses.

Athymic mice grafted with antigen-reactive cloned T cells and challenged with Plasmodium chabaudi adami produced antibodies against multiple parasite antigens. Antibody reactivities were similar to those seen in infected euthymic mice and contrasted with their absence in infected athymic mice. These results suggest that it will not be possible to determine the antigenic specificity of clonal T cell populations in malarial infections by their capacity to provide help to restricted populations of B lymphocytes.

Animals↗

PubMed1983

Evidence that the multifunctional polypeptides of vertebrate and fungal fatty acid synthases have arisen by independent gene fusion events.

The enoyl reductase (NADPH binding site) of rabbit mammary fatty acid synthase has been radioactively labelled using pyridoxal phosphate and sodium [3H]borohydride. Using this method we have been able to add this site to the four sites whose location has already been mapped within the multifunctional polypeptide chain of the protein. The results show that the enoyl reductase lies between the 3-oxoacylsynthase and the acyl carrier. This confirms that the active sites occur in a different order on the single multifunctional polypeptide of vertebrate fatty acid synthase and the two multifunctional polypeptides of fungal fatty acid synthase, and suggests that these two systems have arisen by independent gene fusion events.

Animals↗