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Biomedical subjects

J P Gusdon

Publications and source records attributed to J P Gusdon.

At least 19 recordsLinked to original sources
PubMed1989

Retroperitoneal mucinous cystadenoma.

Twelve cases of retroperitoneal mucinous cystadenoma or mucinous cystadenocarcinoma in women have been reported in the literature. We report an additional case. The histogenesis of mucinous cystadenomas and mucinous cystadenocarcinomas in the retroperitoneum is not clear, but that these lesions may arise from teratomas, ectopic supernumerary or accessory ovaries, or coelomic metaplasia has been suggested. It is possible that small clusters of coelomic epithelial cells could be deposited along the route of ovarian embryonic descent and, by proliferation or metaplastic differentiation, develop into these cystic tumors. The histogenesis of the rare histologically similar lesions that have been seen in men is even less clear.

Adult↗
PubMed1985

Immunotherapeutic studies of different clonal lines of herpesvirus-induced fibrosarcoma in a syngeneic rat.

Transformation of White Buffalo rat embryonic cells by dye-light-inactivated herpesvirus type 2 resulted in the development of two clones of transformed cells (G2 and rat fibrosarcoma) with significantly different tumorigenic capabilities. The G2 cell line was initially nontumorigenic, while the rat fibrosarcoma line was very highly tumorigenic in rats. These significant differences in transformed cell clones from the same initial culture offered an opportunity to study aspects of the immunobiology of oncogenicity. The development of fibrosarcomas in immunoincompetent nude mice with the same early passages of the G2 cell line which were nontumorigenic in the immunocompetent rat suggested that immunologic resistance developed more effectively in the competent host against the G2 line than against rat fibrosarcoma cells. Syngeneic rats which were first exposed to the early passage nontumorigenic G2 cells were completely protected against tumor development by the rat fibrosarcoma cell lines. In subsequent in vitro passages of the G2 cell line, it lost its ability to protect against rat fibrosarcoma challenge and gradually became oncogenic in rats. Modification of antigenic exposure, accomplished by treating the G2 cells with cholesteryl hemisuccinate, resulted in an increase in the protection by these cells and a delay in tumor development.

Animals↗
PubMed1978

Evaluation of the shake test to predict respiratory distress syndrome.

An analysis of the relationship of amniotic fluid shake test titers and the subsequent fetal lung maturity as evinced by the development of the respiratory distress syndrome (RDS) has been conducted. Over a four-year period, 131 amniotic fluid samples were tested within 48 hours of delivery. RDS was diagnosed in 16 infants. When the shake test was positive at a 1:2 dilution, one child developed a mild case of RDS (2.3%). If the test was postivie at a 1:4 dilution, none developed RDS. RDS occurred in 15% of the cases with a positive test at a 1:1 dilution and in 30% of the cases with a negative test. This test has proved to be an excellent screening method for predicting fetal lung maturity if it is positive at 1:2 or greater. If the result is positive at a 1:1 dilution, or negative, other methods must be used for assessment. Its advantages are its rapidity, simplicity, and low cost.

Amniotic Fluid↗
PubMed1977

Studies of lymphocyte populations in pre-eclampsia-eclampsia.

The possibility that the etiology of toxemia might be immunologic has been held for over 70 years. In the past decade, numerous studies have been instituted in attempts to verify the possible role of the immune system in this disease. The present study was undertaken as a probe to determine if a gross difference in the numbers of T and B cell lymphocyte populations might exist between pre-eclamptic and normally pregnant women. Twenty-five normally pregnant women in the third trimester were compared to 25 pre-eclamptic women, and significant differences were noted. If, indeed, there is an immunologic basis for pre-eclampsia, it is more subtle than the methodology used in this study is capable of detecting.

B-Lymphocytes↗
PubMed1977

A clinical evaluation of the "roll-over test" for pregnancy-induced hypertension.

A study of the capability of the roll-over test to predict pregnancy-induced hypertension was undertaken using our private patients. Sixty primigravid and 60 multigravid patients were studied between the twenty-eighth and thirty-second week of gestations. These patients were chosen at random by one of our two nurses who conducted all these studies. All results were recorded but were not available to the physicians. Eighteen months later, after all study patients had delivered, the hospital charts and the patients' office records were evaluated to determine if preganancy-induced hypertension had occurred. In primigravid patients a positive test accurately predicted the later development of pregnancy-induced hypertension only 50 per cent of the time while a negative test accurately predicted that it would not develop 93 per cent of the time. In multigravid patients, only 25 per cent of the patients who had positive tests later developed hypertension. The negative test in multigravid patients was accurate 94 per cent of the time.

Blood Pressure Determination↗
PubMed1977

IgA activation.

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Complement Fixation Tests↗
PubMed1976

The effect of active immunity against placental proteins on pregnancy in monkeys.

Squirrel monkeys were actively immunized against human placental lactogen (HPL) and/or an extract of human placental tissue, or squirrel monkey placenta. Only half of the monkeys immunized with HPL developed a detectable antibody titer to HPL prior to the subsequent mating season. During the subsequent breeding season, the pregnancy rate was cut in half. The titer developed against HPL did not seem to be related to their ability to become pregnant. When the group of animals which had previously been immunized with HPL were challenged with extracts of human placenta, all animals developed detectable circulating antibody. There was no increase in the effectiveness of this immunization as compared to HPL in pregnancy prevention, however. A third group of monkeys were immunized with extracts from their own placental extracts, and all developed some degree of antibody. The titer obtained did not seem to be related to whether or not they became pregnant. In this group, however, the pregnancy rate was decreased to one fourth of what it had been before immunization.

Animals↗
PubMed1976

Purification of naturally occurring antibody to human placental lactogen by affinity chromatography.

Antibodies against human placental lactogen (HPL) were isolated from maternal postpartum sera and nonpregnant female sera by the technique of immunoadsorption (or affinity chromatography). HPL was linked to Sepharose gel by cyanogen bromide activation and the resultant chromatographic resin was used in a repetitive column procedure to absorb and fractionate naturally occurring antibodies to HPL. The antibody to HPL was concentrated after elution from the column with a chaotropic ion, and tested for immunologic acitivity. It was determined to be an IgG molecule, and only radioimmunoelectrophoresis was sensitive enough, among the several tests performed, to show specific anti-HPL activity after treatment.

Adolescent↗
PubMed1976

Transformation of human embryonic fibroblasts by photodynamically inactivated herpes simplex virus, type 2 at supra-optimal temperature.

Infection of human embryonic fibroblast cell monolayers with neutral red and light-inactivated herpes simplex virus, type 2 (HSV-2) at supra-optimal temperature (42 degrees C) resulted in persistence of viable cells in suspension culture at 37 degrees C which have properties in common with virus transformed cells: formation of cell aggregates, HSV-2-specific antigens and colony formation in soft methyl cellulose medium. These data are consistent with the idea that photodynamic inactivated HSV-2 has potential oncogenic activity.

Antigens, Viral↗