PubMed Health⌕ Search

Biomedical subjects

J P Manning

Publications and source records attributed to J P Manning.

At least 19 recordsLinked to original sources
PubMed2001

Determination of the extracellular concentration of glycine in the rat spinal cord dorsal horn by quantitative microdialysis.

Two quantitative microdialysis methods were used to determine the extracellular concentration of glycine in the dorsal spinal cord of halothane-anaesthetised rats. Extracellular glycine determined by zero net flux was 2.6+/-0.3 microM and by the zero flow method was 3.3+/-0.3 microM. For comparison the glycine content of cerebrospinal fluid was determined to be 6.4+/-1.1 microM. There was no correlation between the extracellular and the cerebrospinal fluid concentrations.

Animals↗
PubMed1989

Thyroid hormone periodicity in healthy adult geldings.

Serum samples were collected from 10 healthy geldings every 4 h for three consecutive days and the triiodothyronine (T3) and thyroxine (T4) concentrations determined by radioimmunoassay. There were significant differences in the hormone concentrations related to time. The mean (+/- sd) T3 concentration peaked around 08.00 h at a level (54.06 +/- 14.02 ng/dl) significantly (P less than .001) higher than the lowest concentration (38.71 +/- 10.81 ng/dl) around midnight. Although the highest mean T3 level was 08.00 h, this value was not significantly different from the noon and 16.00 h levels. Likewise, the mean T3 level at midnight was not significantly different from the 20.00 h and 04.00 h levels, resulting in a plateau from 08.00 h to 16.00 h and a trough from 20.00 h to 04.00 h. The mean (+/- sd) T4 concentration peaked around 16.00 h at a level (2.43 +/- .81 micrograms/dl) significantly (P less than .01) higher than the lowest concentration (1.79 +/- .63 micrograms/dl) around 04.00 h.

Animals↗
PubMed1983

Pathogenic bacteria and fungi associated with extraocular disease in the horse.

In 123 cases of external ocular disease in the horse, pathogenic microorganisms isolated with greatest frequency were Streptococcus sp (43.9%), Staphylococcus sp (24.2%), and Pseudomonas sp (13.8%). Fungi were isolated in 4.8% of the cases. In vitro testing showed that most of the Streptococcus sp isolants were sensitive to ampicillin, cephalothin, and carbenicillin. Most of the Staphylococcus sp isolants were sensitive to gentamicin, cephalothin, and bacitracin. Most of the Pseudomonas sp isolants were sensitive to polymyxin B and gentamicin.

Animals↗
PubMed1983

Decreased tear production associated with general anesthesia in the horse.

Schirmer I tear tests were conducted on 14 horses. The test was performed before and after IV administration of xylazine hydrochloride, during maintenance anesthesia with halothane in oxygen, and 3 hours after discontinuation of anesthesia. Xylazine hydrochloride did not decrease tear production from the mean base-line value of 23.94 +/- 5.23 mm/min after its IV administration. Tear production was decreased to mean values of 15.57 +/- 4.29 mm/min at 30 minutes and 13.84 +/- 4.25 mm/min at 60 minutes during the maintenance of halothane anesthesia. Three hours after anesthesia was discontinued, tear production returned to a mean value of 22.58 +/- 4.12 mm/min.

Anesthesia, General↗
PubMed1982

Liver scintigraphy in ponies.

Six derivatives of ethylenediamine-N,N'-bis (alpha-2-hydroxy phenyl) acetic acid labeled with technetium 99m were prepared and their imaging qualities evaluated in ponies. The 6 agents produced good scintigraphic images of certain structures of the liver in the pony. For each agent, 13 different scans were taken. Dorsal views of the left lateral, right lateral, and quadrate lobe were obtained with dorsal scans. Left lateral and left lateral oblique (45 degrees) scans provided a left lateral view of the left lobe and a medial view of the right lateral lobe. Right lateral scans revealed the right lateral and quadrate lobes. Administration of 99mTc-labeled colloids which are commonly used in other species for liver scintigraphy resulted in extensive lung uptake in the pony.

Animals↗
PubMed1981

A technique for catheterisation of the coronary sinus in adult ponies (Equus caballus).

Long-term catheterisation of the coronary sinus using a specially designed catheter was accomplished in 6 ponies via a right lateral thoracotomy. The catheter comprised a 10 to 12 cm long stiff segment (Teflon) joined to a 100 cm length of pliable medical grade (vinyl) tubing. Catheters were kept functional up to 10 weeks postoperatively. Location of the catheter tip was verified by determining the oxygen tension of anaerobically withdrawn blood samples. Normal values of oxygen tension of the coronary sinus blood in ponies were similar to those reported for the dog, whereas oxygen content was significantly lower.

Animals↗
PubMed1980

Biochemical effects of succinylcholine chloride in mechanically ventilated horses anesthetized with halothane in oxygen.

Succinylcholine chloride administered to horses anesthetized with halothane in oxygen and mechanically ventilated, caused slight but statistically insignificant (P less than 0.01) increases in creatine phosphokinase, lactic dehydrogenase, and aspartate aminotransferase activity. The increases in these enzymes have been explained on the basis of muscle damage resulting from succinylcholine chloride induced muscle fasciculations and by hypoperfusion of tissues due to depression of the cardiovascular system caused by general anesthesia. These changes were not clinically apparent based upon the absence of myoglobinuria and ease of recovery. There was no significant effect of treatment observed on other biochemical variables. The findings in the present study agree with previous observations on serum creatine phosphokinase, lactic dehydrogenase, and aspartate aminotransferase activity.

Alanine Transaminase↗