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J P Persijn

Publications and source records attributed to J P Persijn.

At least 19 recordsLinked to original sources
PubMed1984

Comparison of enzyme assay and radioimmunoassay for the measurement of human acid phosphatase in cases of sexual assault.

We investigated whether a radioimmunoassay for prostatic acid phosphatase might be used as a more specific test for the identification of semen in samples from cases of sexual assault than the measurement of total acid phosphatase enzyme activity. The results of the measurement of acid phosphatase by enzyme assay in semen and vaginal swab extracts were compared with the results of the radioimmunoassay. It was found that the radioimmunoassay is a sensitive and more specific method than the enzymic determination of acid phosphatase. Incidentally we have found that a low concentration of an immunological cross reacting acid phosphatase is present in semen free vaginal swab extracts.

Acid Phosphatase↗
PubMed1984

In search for the best qualitative microscopical or morphometrical predictor of oestrogen receptor in breast cancer.

The correlation between oestrogen receptor (OR), qualitative and quantitative microscopical features has been studied in 198 breast cancers, 60 patients were younger than 50 years of age, 138 were older than 50. This age distinction was made because of the obvious age dependency of the OR-content, with 50 years as the cut-off point. Compared with elastosis grade, the morphometric features have a much lower percentage of doubtful cases (22.2 vs. 40.4%) with a higher percentage overall correct classifications (55.6 vs. 45.4%). In terms of sensitivity and specificity, selection of the best single predictor depends on the age of the patient. Mean nuclear area has the highest combination of sensitivity and specificity (88.9 and 80.0%) in woman younger than 51, while elastosis grade is the best single predictor in patients older than 50 (sensitivity: 91.2%; specificity: 70.6%). Using multivariate analysis, a combination of mean and standard deviation of the nuclear area results in 83.3% correct classifications with only 5% doubtfuls in the younger age group. With more features in the analysis, no false negatives and only 13.3% false positives is the most optimal result. In the older age group, a decision tree consisting of elastosis grade and mean nuclear area gives the best results. Subsequent investigation of mean nuclear area in elastosis grade 0 cancers gives a considerable reduction of the false negatives, thus increasing the specificity to 94.6% and the correct negative predictions to 72.7%. It is concluded that selective morphometry gives a considerable enhancement of the histopathological prediction capacities of oestrogen receptor in breast cancer.

Adult↗
PubMed1982

Carcinoembryonic antigen serum levels in patients with squamous cell carcinoma of the uterine cervix: clinical significance.

In 114 patients with invasive cervical cancer of the squamous cell type pretreatment CEA levels were determined. An individual upper limit of the normal range was derived taking into account the smoking habits and the age of each patient. Pretreatment CEA levels exceeding the upper limit of normal concurred with a very poor prognosis, regardless of the stage of the tumor. Moreover, in 92 patients longitudinal CEA patterns were established. The median follow-up time of the nonrecurrence patients was four years. Three patients appeared to be exclusively associated with the presence of recurrent cancer. The median lead-time obtained in patients demonstrating such patterns, was 13 weeks. The clinical value and the possible therapeutic consequences of the findings presented are discussed in relation to the available knowledge of tumor growth in cervical cancer.

Adult↗
PubMed1982

Estrogen receptors in human breast cancer. II. Correlation between the histochemical method and biochemical assay.

The present study defines criteria for determining the presence of estrogen-receptors in human breast carcinomas demonstrated by a histochemical assay using 17 beta-estradiol-carboxy-methyl-oxim-bovine serum albumen-FITC. The criteria were: 1) the percentage of cells showing fluorescence; 2) the intensity of the fluorescence observed, and 3) the percentage of epithelial structures in tissue specimens. Using these predefined criteria in 132 human breast carcinomas as 91.6% agreement was found between the results of the histochemical assay and those of the biochemical Charcoal method. The main causes of disagreement (7 of the 11 cases) were sampling errors between the tissue specimens used for the histochemical and biochemical assay, and an insufficient percentage of epithelial structures (less than 15%) to allow biochemical identification of estrogen receptor activity. In the hands of pathologists with experience of the field of histochemistry this histochemical assay may be the method of choice for the assessment of estrogen receptors.

Adult↗
PubMed1981

Prognostic significance of CEA in colorectal cancer: a statistical study.

Pretreatment and serial posttreatment carcinoembryonic antigen (CEA) serum levels were studied with respect to the course of disease in 222 patients with colorectal cancer. The CEA values during the subsequent six years were expressed in actuarial or cumulative plots in relation to tumour-free period, time of diagnosis of recurrence and other parameters. The pretreatment CEA value was highly significant but gave no more prognostic information than the Dukes classification. The pretreatment CEA had prognostic significance only in inoperable patients. Elevated pretreatment CEA did not exclude the possibility of curative treatment. Normalization of CEA after resection did not indicate completeness of cure. In patients with local or distant recurrences CEA occasionally rose before recurrences became clinically apparent. Positive lead time was 0-625 days. However, in about 40% of the patients clinical diagnosis of recurrence preceded a rise of CEA. Maximal negative lead time was 585 days. Statistically, recurrence without a rise of CEA was exceptional. The results strongly suggest that serial CEA determinations cannot replace physical examination and follow-up.

Adult↗
PubMed1980

Automated method for the determination of 5'-nucleotidase in serum by continuous flow analysis.

The manual method of Persijn & Van der Slik (J. Clin. Chem. Clin. Biochem. 6, 441-446 (1968): 7, 493-497 (1969); 8, 398-402 (1970) for the determination of serum 5'-nucleotidase has been adapted to the Auto-Analyzer II System. In the Auto-Analyzer II, the incubation temperature for the enzyme reactions is 37 degrees C, and the effective sample speed is 30/h, at a sample/wash ratio of 2:1. There is good agreement and correlation between the manual method and the Auto-Analyzer II method (equation of regression line: y = x + 0.6, correlation coefficient = 0.988; the normal range is 2.9-10.5 U/l for both methods). In routine use, within-run and between-day reproducibility are considerably smaller for the Auto-Analyzer II than for the manual method, especially when large numbers of samples are assayed.

5'-Nucleotidase↗
PubMed1979

Urinary beta 2 microglobulin in upper and lower urinary-tract infections.

Beta 2-- microglobulin excretion in 24 h urine collections and beta2- microglobulin clearance were significantly increased in patients with upper-urinary-tract infections, but beta 2- microglobulin clearance and excretion were normal in patients with cystitis. Beta 2-microglobulin estimation may be used to distinguish between upper and lower urinary-tract infections, except when pre-existing tubular damage is present. Voided urine must have a pH above 6 . 0, since beta 2- microglobulin is rapidly degraded in the bladder at low pH.

Aged↗
PubMed1979

A rapid enzymatic assay for methotrexate in serum.

A kinetic enzymological assay for methotrexate in serum is described; the results are available after 1.5 hours. The lower limit of sensivity is 50 nmol/1; the day-to-day variation coefficients compare favourably with other procedures (about 7% in the 200-600 nmol/1 range). The method uses purified dihydrofolic acid reductase from bovine liver. The problem of its instability in the assay is circumvented by several measures, e.g. the use of a reaction rate analyser.

Humans↗
PubMed1978

On receptors for estrogens (E2) and androgens (DHT) in human endometrial carcinoma and ovarian tumours.

Some human endometrial carcinomas contain receptors for estrogen (E2) and dihydrotestosterone (DHT). Analysis of 50 primary endometrial carcinomas by agar gel electrophoresis revealed a varying receptor pattern, absence of both receptors, sole presence of E2- or DHT- receptor, or presence of both. Highly differentiated tumours often had receptors of both types (7/18) and poorly differentiated tumours most often lacked them (1/10). Primary tumours with metastases (Stages III-IV) seldom had combined receptors, only in 2 cases out of 9. Growth of the tumour down in the cervical canal (Stage II) implied absence of combined receptors in all of the 13 cases studied. In 8 different ovarian tumours, presence of either E2- or DHT-receptors was found in 6. The findings are discussed in relation to hormonal treatment of the disease.

Female↗