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J Pérez Losada

Publications and source records attributed to J Pérez Losada.

6 recordsLinked to original sources
PubMed1998

Effect of cyclosporin A on inflammatory cytokine production by human alveolar macrophages.

Cyclosporin A (CsA) is an immunomodulator drug that has been used in the treatment of several types of advanced pulmonary interstitial disease. This beneficial effect occurs mainly in circumstances in which alveolitis due to CD4 lymphocytes is absent, suggesting that CsA acts on other types of cells. The present study was undertaken to determine the effect of CsA on inflammatory cytokine secretion by human alveolar macrophages (AMs). Human AMs were collected by bronchoalveolar lavage from four control subjects and 13 patients with interstitial lung disease. Purified human AMs were incubated with different concentrations of CsA (200, 20 and 2 ng ml-1) in the presence or absence of lipopolysaccharide (LPS). Interleukin-1 beta (IL-1 beta), tumour necrosis factor-alpha (TNF-alpha), IL-6 and IL-8 levels were measured in supernatants using specific enzyme-linked immunosorbent assays. It was found that CsA inhibits basal secretion of TNF-alpha and IL-8 at 20 and 200 ng ml-1. However, none of the different concentrations of CsA modified basal secretion of IL-1 beta nor IL-6. By contrast, a lower concentration of CsA (2 ng ml-1) inhibits LPS-stimulated secretion of all inflammatory cytokines. It is concluded that CsA exerts a modest effect on inflammatory cytokine production by human AMs.

Alveolitis, Extrinsic Allergic↗
PubMed1985

[The tobacco habit among children].

The authors investigate the level of tobacco consumption in 240 school boys and girls between the ages of 8 and 14 in an urban and semiurban area of Galicia. 29% of the school children in Galicia smoke tobacco and 19.6% are habitual smokers. The greatest percentage of smokers are: males 35.6% boys and 23% girls, between the ages of 12 and 14, children from working class backgrounds and children with lower academic progress. The habit of smoking practised outside the home is associated in a high percentage of cases to the consumption of alcohol (65% of the smokers drink alcohol habitually). The consumption of tobacco is greater in children with parents that smoke. Only 1.25% of the sample smoke marihuana habitually.

Adolescent↗