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J Panella

Publications and source records attributed to J Panella.

10 recordsLinked to original sources
PubMed2004

Analysis of the accuracy of linear measurements obtained by cone beam computed tomography (CBCT-NewTom).

OBJECTIVES: The conical beam computed tomography (CBCT) technique presents an innovation of tomographic imaging systems and subsequent volumetric image reconstruction for dentistry. When compared with other methods of tomographic imaging CBCT is characterized by rapid volumetric image acquisition from a single low radiation dose scan of the patient. The NewTom (NewTom 9000; Quantitative Radiology, Verona, Italy) is an example of such a CBCT machine dedicated to dental and maxillofacial imaging, particularly for surgical and/or prosthetics implant planning in the field of dentistry. The aim of this study was to evaluate the accuracy of the linear measurements obtained in CBCT images using a NewTom. METHODS: Thirteen measurements were obtained in dry skulls (n = 8) between internal and external anatomical sites using a caliper. These were considered as real measurements. Then the dry skulls were submitted to CBCT imaging examinations. Radiographic distance measurements of the same dry skull anatomical sites were made using the NewTom QR-DVT 9000 software of the 2 mm-CTs axial section images and sagittal or coronal reconstructions. The data were compared by paired Student's t-test. RESULTS: The results showed that the real measurements were always larger than those for the CBCT images, but these differences were only significant for measurements of the internal structures of the skull base. CONCLUSIONS: The conclusion of this study is that, although the CBCT image underestimates the real distances between skull sites, differences are only significant for the skull base and therefore it is reliable for linear evaluation measurements of other structures more closely associated with dentomaxillofacial imaging.

Cephalometry↗
PubMed2004

Mandibular ramus-related Stafne's bone cavity.

Mandibular bone depressions located on the lingual/buccal aspect of the mandibular ramus are the rarest variants of the so-called Stafne's bone cavities, or major salivary gland-related depressions, with only 17 cases reported in the literature including both clinical cases and archaeological specimens. We report the case of a 14-year-old male patient who sought clinical assistance complaining of a hard expansion on the lower left premolar-molar region. Apart from a unilocular radiolucent lesion between the lower left second premolar and first molar, a panoramic radiograph showed another radiolucent lesion located in the right mandibular ramus, at the level of the mandibular foramen. Computed tomography (CT) revealed an expansile lesion in the left mandibular body, later diagnosed as a simple bone cyst through surgical exploration. The three-dimensional CT volume rendering reconstructed image showed that the second lesion, located on the lingual aspect of the ascending ramus, was an actual cortical bone defect, which was diagnosed as a mandibular ramus-related Stafne's bone cavity. Considering the young age of the patient, the size of the defect, the recognizedly slow development of mandibular bone defects and, above all, the location of the bone defect under discussion, we believe it to have a congenital rather than a developmental origin (i.e. it was caused by a focal failure during intramembranous ossification of the mandible). If this is the case, mandibular bone depressions should not be seen exclusively as salivary gland-related bone defects.

Adolescent↗
PubMed1998

In vivo expression of an alternatively spliced human tumor message that encodes a truncated form of cathepsin B. Subcellular distribution of the truncated enzyme in COS cells.

Cathepsin B is a lysosomal cysteine protease whose increased expression is believed to be linked to the malignant progression of tumors. Alternative splicing and the use of alternative transcription initiation sites in humans produce cathepsin B mRNAs that differ in their 5'- and 3'-untranslated ends. Some human tumors also contain cathepsin B-related transcripts that lack exon 3 which encodes the N-terminal signal peptide and 34 of the 62-amino acid inhibitory propeptide. In this study we show that one such transcript, CB(-2,3), which is missing exons 2 and 3, is likely to be a functional message in tumors. Thus, CB(-2,3) was found to be otherwise complete, containing the remainder of the cathepsin B coding sequence and the part of the 3'-untranslated region that is common to all previously characterized cathepsin B mRNAs in humans. Its in vitro translation product can be folded to produce enzymatic activity against the cathepsin B-specific substrate, Nalpha-benzyloxycarbonyl-L-Arg-L-Arg-4-methylcoumaryl-7-amide. Endogenous CB(-2,3) from the metastatic human melanoma cell line, A375M, co-sediments with polysomes, indicating that it engages the eukaryotic translation machinery in these cells. Epitope-tagged forms of the truncated cathepsin B from CB(-2,3) are produced in amounts comparable to the normal protein after transient transfection into COS cells. Immunofluorescence microscopy and subcellular fractionation show this novel tumor form of cathepsin B to be associated with nuclei and other membranous organelles, where it is likely to be bound to the cytoplasmic face of the membranes. This subcellular distribution was different from the lysosomal pattern shown by the epitope-tagged, full-length cathepsin B in COS cells. These results indicate that the message missing exons 2 and 3 is likely to be translated into a catalytically active enzyme, and that alternative splicing (exon skipping) could contribute to the aberrant intracellular trafficking of cathepsin B that is observed in some human cancers.

Alternative Splicing↗
PubMed1979

Ultrasound detection of a carcinoid tumor.

The intraluminal component of symptomatic malignant carcinoid tumors of the small bowel is typically small relative to the extension in the mesentery, and barium examinations are occasionally normal. This case report illustrates the diagnostic value of abdominal ultrasonography in the detection of extraluminal component of a carcinoid tumor.

Carcinoid Tumor↗