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J Papillo

Publications and source records attributed to J Papillo.

5 recordsLinked to original sources
PubMed1998

Performance optimization of the ThinPrep processor: effect of microscope slides.

Our objective was to evaluate the cellular adherence property of various microscope slides. Slide preparations were made using the ThinPrep 2000 System, in which cells are transferred from a filter to a slide. A total of eight slide types was selected for testing against the ThinPrep microscope slide: S/P MicroSlide, S/P Pusher, S/P Colorfrost, Menzel Superfrost, Sayag, Sail Brand, Fisher Plus, and Newcomer Poly-L-Lysine. Gynecologic samples from 4 different patients were utilized on the ThinPrep Processor. Multiple preparations were made from each sample using the different microscope slides. The slides were stained with a commercial Pap stain, and cellular material was quantified by scanning each preparation using a linear stage mounted on a Hitachi U-2000 spectrophotometer set at 580 nm. The data generated by this study show that ThinPrep microscope slides produce preparations with the highest level of cellular material. This study quantifies slide performance, examines a range of microscope slide types, and shows that ThinPrep microscope slides exhibit significantly increased cell adherence over the other slide types tested.

Cell Adhesion↗
PubMed1991

Flow cytometric DNA analysis versus cytology in the evaluation of peritoneal fluids.

The use of flow cytometric DNA analysis as an adjunct to cytology in peritoneal fluid evaluation was studied. One hundred ninety-five fluids from 193 gynecologic patients were subjected to both DNA analysis and cytologic examination. It was found that 117/195 (60%) had invasive malignancies (50 ovarian, 48 endometrial, 17 cervical, and 2 miscellaneous); 34/117 (28%) patients with malignancies were positive by cytology, and 10/117 (8.5%) were positive (aneuploid) by DNA analysis. Of 34 cytologically positive cases, 7 (21%) were DNA positive, 25 (74%) were DNA negative, and in 2 (6%) insufficient cells were obtained. Only 3 fluids (3%) from malignancies were positive by flow cytometry and negative by cytology (1 stage I ovarian cancer, 1 stage I endometrial cancer, and 1 stage III ovarian cancer). No false-positive cytology and one probable false-positive flow result was obtained. If only those patients with histologically documented peritoneal involvement are considered, 29/43 (65%) had positive cytology and 8/43 (19%) had a positive flow result. We conclude that: (1) the high false-negative rate of flow cytometry (79%) versus cytology in this study may be related to a high percentage of diploid cancers, specimen preparation, or histogram interpretation, and (2) flow cytometry rarely adds to cytologic evaluation and is probably best reserved for use only in selected cases.

Ascitic Fluid↗
PubMed1986

False-negative peritoneal cytology in metastatic ovarian carcinoma.

Seventy-nine laparotomies for disseminated intraperitoneal ovarian carcinoma were reviewed to determine the frequency and possible causes of false-negative peritoneal cytology. Negative peritoneal cytology (defined as any reading other than positive) was found in 16 of 79 cases (20%). False-negative cytology occurred more frequently with peritoneal washings (48%) than with ascites (6%; P less than .001); with second-look surgery (50%) than at primary surgery (12%; P = .004); with peritoneal metastasis less than 0.5 cm (50%) than with metastasis greater than 0.5 cm (16%; P = .02); and with bloody cytology specimens (25%) rather than specimens without blood (0%; P = .06). Volume of peritoneal specimen, architectural grade, cytologic grade, and stage of disease (III versus IV), did not affect the frequency of false-negative cytology. The high prevalence of negative cytology associated with peritoneal washings, small tumor size, and second-look surgery suggests that negative cytology is a result of poor distribution of peritoneal washings and infrequent exfoliation of malignant cells rather than misinterpretation of malignant cells present in cytology specimens.

Ascitic Fluid↗
PubMed1985

The effect of cytoxan, adriamycin, and cis-platinum on cervical, vaginal cytology.

One hundred and thirty-four cervical and vaginal smears were obtained from 51 patients receiving cyclophosphamide, doxorubicin, and cis-platinum. These drugs do not appear to cause dysplasia or significant atypia. Additionally, the Pap smear was not useful for the diagnosis of unexpected persistent or recurrent carcinoma of the ovary.

Adenocarcinoma↗