Biomedical subjects

J Pasantes

Publications and source records attributed to J Pasantes.

3 recordsLinked to original sources

PubMed1989

Denaturing effect of acridine orange and adriamycin.

Acridine orange and adriamycin have a very low denaturing effect on DNA isolated from Chinese hamster cells but strongly increase heat-induced denaturation. When these intercalating agents are added to the growth media they modify the sensitivity to heat denaturation of DNA extracted from treated cultures when compared with untreated cultures. This effect correlates with a considerable increase in the disruption of chromosomal ultrastructure.

Acridine Orange↗

PubMed1988

Changes in metaphasic chromosomal ultrastructure caused by denaturing treatments.

Denaturation and posterior renaturation of metaphase chromosomes after different treatments were tested by acridine orange staining. A relationship between conservation of ultrastructure and the capacity to renature was discovered. Amyl acetate, commonly used in electron microscopy to fix chromosomes, avoids the action of denaturing agents and this effect is not reversed by chromosome rehydration.

Animals↗

PubMed1987

Morphological parameter variations between sister chromatids by electron microscopy.

Five morphological parameters of sister chromatids, viz radius, length, centromeric index, volume and centromere width, were studied by electron microscopy, from 140 human number 1 chromosomes, and 122 human number 2 chromosomes. The degree of variation obtained ranged from 0.277 +/- 0.003% and 0.286 +/- 0.005% for the centromeric index of chromosomes 1 and 2, respectively, to 9.835 +/- 0.933% and 13.472 +/- 1.461% for centromere width.

Cells, Cultured↗