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J Patterson-Delafield

Publications and source records attributed to J Patterson-Delafield.

6 recordsLinked to original sources
PubMed1981

Microbicidal cationic proteins of rabbit alveolar macrophages: amino acid composition and functional attributes.

We purified two microbicidal cationic proteins, MCP-1 and MCP-2, from rabbit alveolar macrophages. MCP-1 was remarkably rich in arginine (25.5 mol%) and half cystine (18.7 mol%) residues and constituted approximately 1.5% of the total protein content of Freund adjuvant-elicited alveolar macrophages. MCP-2 was approximately half as abundant as MCP-1 and contained relatively less arginine (14.9 mol%) and half cystine (9.8 mol%). The amino acid compositions of MCP-1 and MCP-2 resembled those reported for the lysosomal cationic proteins of rabbit granulocytes, but were distinct from those of any known histone. MCP-1 (1 microgram/ml) killed 99.6% of Candida albicans in 20 min, whereas MCP-2 killed approximately 80% under similar conditions. Both proteins rapidly suppressed O2 consumption by C. albicans and induced a rapid loss of intracellular 86Rb+. Although more information is needed about the biological origin, distribution, and roles of macrophage microbicidal proteins, it seems likely that MCP-1 and MCP-2 contribute to the microbicidal efficacy of rabbit alveolar macrophages.

Amino Acids↗
PubMed1980

Preparation of rabbit alveolar macrophages in high purity and yield.

We describe methods that permit large numbers of viable and functional alveolar macrophages (greater than or equal to 5 x 10(8)/rabbit), substantially free of granulocyte contamination, to be recovered. Such populations are especially well suited for biochemical analyses demanding cell purity.

Animals↗
PubMed1980

Fungicidal activity of rabbit alveolar and peritoneal macrophages against Candida albicans.

We tested the ability of rabbit macrophages to kill Candida albicans in vitro. Resident (unstimulated) alveolar macrophages killed 28.1 +/- 1.9% of ingested organisms in 4 h, whereas resident peritoneal macrophages killed only 15.2 +/- 1.3% (mean +/- standard error of the mean, P < 0.01). Peritoneal macrophages obtained from rabbits treated 3 weeks earlier with complete Freund adjuvant showed enhanced candidacidal activity relative to normally resident peritoneal cells (28.2 +/- 3.1%, P < 0.01). Candidacidal activity by alveolar macrophages recovered from such treated animals was slightly enhanced relative to untreated alveolar macrophages (32.9 +/- 2.3%). Candidacidal activity by peritoneal and alveolar macrophages was not decreased by several agents (cyanide, azide, sulfadiazine, and phenylbutazone) that inhibit the ability of human blood monocytes to kill C. albicans. In contrast, candidacidal activity by alveolar macrophages was greatly diminished by iodoacetate, an ineffective inhibitor of this function in human monocytes. We conclude that rabbit macrophages kill C. albicans by a fungicidal mechanism distinct from the peroxidase-H2O2 mechanism of human granulocytes and monocytes, and that the fungicidal properties of peritoneal and alveolar macrophage populations are enhanced after nonspecific stimulation with complete Freund adjuvant.

Animals↗
PubMed1980

Microbicidal cationic proteins in rabbit alveolar macrophages: a potential host defense mechanism.

Rabbit alveolar macrophages contain two highly cationic microbicidal proteins. These were shown to be distinct from histones and not to arise from granulocyte contamination. The macrophage proteins were especially active against Candida albicans and Candida parapsilosis. Gram-positive bacteria (Bacillus subtilis, Listeria monocytogenes, and Streptococcus faecalis) were also susceptible, whereas Escherichia coli and Salmonella typhimurium appeared more resistant. The proteins may be present in lysosomes, based on their solubilization by dilute acids and their distribution with lysosomal markers on sucrose density gradients. Such microbicidal proteins have not previously been demonstrated in any mammalian macrophage. They may play a significant role in the host-defense functions of the rabbit lung.

Animals↗