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Biomedical subjects

J Peacock

Publications and source records attributed to J Peacock.

70 records · Page 4Linked to original sources
PubMed1986

Infectious endophthalmitis.

A clinical series of twenty-eight cases of suspected infectious endophthalmitis was reviewed. The combination of epidemiology and peroperative microscopy at the time of vitreous biopsy provided a useful indication of the pathogen involved. The visual prognosis was related to the virulence of the pathogen, and to the delay between recognition of symptoms or signs and treatment. It appears possible to predict high risk groups which warrant a high index of clinical suspicion and prompt diagnosis to enable early vitrectomy with injection of appropriate intraocular antibiotics.

Endophthalmitis↗
PubMed1985

Transformation associated p53 protein is encoded by a gene on human chromosome 17.

The human gene for the transformation-associated p53 phosphoprotein (P53) was assigned to the short arm of chromosome 17 using human-rodent somatic cell hybrids and Southern filter hybridization of cell hybrid DNA. The filters were hybridized to radiolabeled DNA from a genomic clone which contained P53 nucleotide sequences. Hybridization of the probe to a 2.5-kb human DNA fragment in HindIII-digested DNA was used to identify the human P53 gene.

Animals↗
PubMed1984

Isolation and characterization of a human p53 cDNA clone: expression of the human p53 gene.

A cDNA clone for human p53 cellular tumor antigen has been isolated and characterized. This clone contains the complete 3'-untranslated region and most of the open reading frame for the protein. Nucleotide sequence analysis revealed that p53 mRNA contains an Alu repeat in the 3'-untranslated region. Hybridization selection experiments showed this clone was capable of selectively binding p53 mRNA. In vitro translation of SV80 mRNA resulted in the synthesis of two immunoreactive p53 polypeptide species. Northern blot analysis showed that human p53 mRNA was 2.8 kb in length and was present in cell lines containing high and low levels of p53 protein. There appears to be only a single p53 gene in human cells and Southern blot analysis demonstrated no major genomic rearrangements or amplification of the p53 gene in the transformed cell lines examined.

Antigens, Neoplasm↗
PubMed1981

Moraxella keratitis in a nonalcoholic population.

Moraxella keratitis has been commonly described as a disease of debilitated older patients, particularly malnourished chronic alcoholics. This communication reviews the features of corneal infection due to Moraxella nonliquifaciens occurring in both young and old patients in otherwise good general health. Chronic corneal epithelial disturbance was a common finding in the 8 patients reported, indicating that more localised corneal debilitation may have a role in the pathogenesis of this disease.

Adult↗
PubMed1981

Gel layer limited haemofiltration rates can be increased by vortex mixing.

Haemofiltration has been used since 1976 as an alternative to haemodialysis in the treatment of chronic renal failure (1). Ultrafiltration flow rates (UFR) from blood are slow and it has been observed that when blood or plasma is ultrafiltered using highly permeable membranes, the UFR reaches a maximum which is independent of trans-membrane pressure (delta Pm) (2). We have confirmed this result during steady flow through an ultrafilter employing polyacrylonitrile membrane (Rhône Poulenc AN 69) but have also shown that this maximum can be exceeded by a factor of eight in the same device when vortex mixing is induced by pulsing the blood flow over furrowed channels.

Blood↗
PubMed1980

Branhamella keratitis.

Three cases of suppurative keratitis caused by Branhamella catarrhalis are described. Each presented as a localised stromal infiltrate in a previously scarred cornea. The condition responded to penicillin G and to gentamicin treatment.

Bacterial Infections↗
PubMed1977

Synapse formation between two clonal cell lines.

Clonal neuroblastoma x glioma hybrid cells frequently formed synapses with clonal mouse striated muscle cells. Clonal myotubes were similar to cultured mouse embryo myotubes with respect to acetylcholine sensitivity and other membrane properties examined. However, acetylcholine sensitivity measurements indicate that acetylcholine receptors of clonal myotubes are distributed more uniformly over the cell surface than the receptors of cultured mouse embryo myotubes.

Acetylcholine↗
PubMed1976

Myosin synthesis increased by electrical stimulation of skeletal muscle cell cultures.

When cultures of skeletal muscle cells of the chick embryo are subjected to repetitive, electrical stimulation, the contractions increase the amount of protein produced by these cells. The increase is greater for contractile proteins such as myosin heavy chain than for total cellular protein. This demonstrates that in a culture system of skeletal muscle cells that have differentiated in the absence of innervation, one can elicit the protein synthetic response associated with skeletal muscle hypertrophy in vivo.

Animals↗
PubMed1972

An active electrical response in fibroblasts.

L cells have a resting potential of about -16 mv (internal negative) at 37 degrees C in Dulbecco's modified Eagle's medium containing 10% fetal calf serum and a potassium concentration of 5.4 mM. Membrane resistivity is about 20,000 ohmcm(2) when the surface filopodia described by others are taken into account. Mechanical and electrical stimuli can evoke an active response from mouse L cells, cells of the 3T3 line, and normal fibroblasts which we have termed hyperpolarizing activation or the H.A. response. This consists of a prolonged (3-5 sec) increase in the membrane permeability by a factor of 2-10 with a parallel increase in membrane potential to about -50 mv. The reversal potential for the H.A. response is -80 mv. The resting cells are depolarized to about -12 mv when the external medium contains 27 mM potassium, and the potential reached at the peak of the H.A. response is about -30 mv. The reversal potential for the H.A. response is about -40 mv in 27 mM external potassium. This effect of potassium ions on the reversal potential of the H.A. response leads us to conclude that the response represents an increase in membrane permeability, predominantly to potassium, by at least a factor of five. This increase must be greater than 20-fold if previous measurements of the ratio of potassium permeability to chloride permeability in L cells are valid for the preparation used in the present study.

Animals↗
PubMed1971

Genes for neuronal properties expressed in neuroblastoma x L cell hybrids.

Neuroblastoma cells with electrically excitable membranes were fused with electrically passive L cells having a hitherto undescribed electrical marker. Hybrid cells, examined 10-40 generations after fusion, were found to be electrically excitable. The results show that at least a part of the genetic information for neuron differentiation can be functionally expressed in N x L hybrid cells. Evidence for the regulation of action potential components was also found.

Action Potentials↗
PubMedDate not supplied

The incorporation and release of bovine serum albumin from poly-hydroxybutyrate-hydroxyvalerate microcapsules.

Spherical microporous reservoir type microcapsules composed of P(HB-HV) (10.8% HV)/20% PCL containing BSA (surrogate protein) loaded agarose have been fabricated using a double emulsion technique with solvent evaporation. Microcapsules were generated in high yield (> 75 wt%) and BSA incorporation had no significant effect on microcapsule size distribution (21-200 microns). The loss of BSA both by partitioning into the aqueous continuous phase, and through micropores as BSA-loaded-agarose during the precipitation of the fabrication polymer concomitant with solvent evaporation, resulted in low encapsulation efficiency (12%). The amount and duration of BSA release was influenced as much by micropore numbers and diameter as by the extent of reservoir loading and detectable levels of BSA release could be monitored for up to 24 days.

Animals↗