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J Pelnár

Publications and source records attributed to J Pelnár.

4 recordsLinked to original sources
PubMed1997

1H NMR ganglioside ceramide resonance region on the differential diagnosis of low and high malignancy of brain gliomas.

1. The high-resolution 1H NMR (MRS) spectra of human brain tumor homogenates revealed a broad resonance at 5.3-5.4 ppm in glioblastoma multiforme (N = 16) and brain metastases (N = 3). The broad resonance was identified as ceramide, a sphingosine-fatty acid combination portion of ganglioside, indicating an elevated abundance of monounsaturated fatty acids. GLC analysis of gangliosides in the highly malignant glioblastoma multiforme revealed that the elevated monounsaturated fatty acid is oleic acid (C18:1). The resonance at 5.3-5.4 ppm region was not detectable in normal human brain (N = 2), in meningiomas (N = 2), or in low-grade astrocytomas (N = 12). In normal human brain the abundance of monounsaturated fatty acid is minimal. 2. This investigation was made possible because the method of producing homogenate resulted in (i) no loss of lipids during the process and (ii) a well-homogenised sample, with (iii) no loss in chemical integrity. 3. The properties of tumor gangliosides include antigenic specificity and immunosuppressive activity and the ceramide, a sphingosine-fatty acid combination, noticeably influences the ganglioside immunosuppressive activity. 4. The observation of 1H NMR ceramide resonance in high-malignant brain tumors emphasizes the dramatic role of aberant gangliosides and ceramide precursors on the grade of malignancy and invasiveness. 5. Further insight into the specific nature of the ceramide portion of gangliosides in grading the malignancy of brain tumors should be investigated further.

Astrocytoma↗
PubMed1989

[Unusual complications of anticoagulation therapy].

In the introduction to the work the authors deal with the problems of the prevention of flebotromboses in patients operated on in the area of proximal part of the femur. They analyze ways of physical and pharmacological prevention and point out the potential undesirable effects and unusual complications in the treatment by means of Heparin and Kumarin derivatives--and namely the toxoallergic reaction to Heparin and the necrosis of skin and subcutis in case of the application of Kumarin drugs. In Kumarin necrosis the etiology of the which has not been, unequivocally elucidated, yet, they present views on the causes of its origin and describe the course of the Kumarin necrosis. They summarize predispositional factors which increase the risk of its occurrence and present their own observations of two cases. In the discussion they deal with the views on the etiology of the origin of this complication. In the conclusion they recommend the treatment in compliance with the latest findings.

Aged↗
PubMed1989

[The Immunoskintest].

To evaluate the surgical risk before serious operations it is possible to use as one of the criteria a skin test the principle of which is an immunity reaction to a defined antigen injected strictly intradermally. It helps us to assess simply the state of immunological reactivity of the cellular type in a large number of persons of different age and thus to estimate to a certain extent the incidence of postoperative infectious complications. In a selected group of 68 patients who were hospitalized at the Orthopaedic Clinic in Plzen in 1987 the Immunoskintest was performed. The authors compare the incidence of infectious complications in different groups by the character of the disease, i.e. in a) planned operations in coxarthritis, b) in patients with injuries of the proximal part of the femur immediately after injury, c) in patients with injuries of the proximal part of the femur treated conservatively. The incidence of infectious complications is evaluated in conjunction with the results of the Immunoskintest.

Aged↗