Biomedical subjects
J Perez de la Lastra
Publications and source records attributed to J Perez de la Lastra.
Ruminant cluster WC13.
Five monoclonal antibodies (mAbs) belonged to preliminary cluster 27; however, only two mAbs, Buf13 (3W-506) and Co-3D1D4 (3W-202), were shown to detect the same surface antigen and belong to WC13. The other three mAbs, IVA120 (3W-323), IVA197 (3W-533) and IVA198 (3W-290), detected a 45 kDa molecule that could be fibrinogen (Mateo A., Perez de la Lastra, J., Moreno, A., Dusinsky, R., Bilka, F., Simon, M., Horovska, L., Naessens, J. and Llanes, D., 1996. Biochemical characterization of antigens detected with anti-platelet monoclonal antibodies. Vet. Immunol. Immunopathol., 52: 363-370; Perez de la Lastra, J.M., Mateo, Dusinsky, R., Bilka, Simon, M., Horovska, L. and Llanes, D., 1996. Two monoclonal antibodies from the platelet panel recognize sheep plasma fibronigen. Vet. Immunol. Immunopathol., 52:).
Biochemical characterization of antigens detected with anti-platelet monoclonal antibodies.
A panel of 18 monoclonal antibodies (mAbs) defined by the third workshop as specific for platelets, clustered in three preliminary groups: PC7, PC13 and PC27. These mAbs were further analysed by immunoprecipitation using extracts of iodinated and biotinylated peripheral blood mononucleated cells (PBMC) and platelets. We could confirm the existence of mAbs with specificities to WC9 (in PC7) and CD41/61 (in PC13). Two mAbs formed a new cluster, WC13, which may be homologous to human CD31 (in PC27). The influence of EDTA and thrombin on the expression of the different antigens on the platelet membrane was assessed by flow cytometry (FCM) analysis, as well as cross-reactivity with platelets from different species.
A monoclonal antibody to ruminant fibrinogen produced through immunization with ovine peripheral blood leukocytes.
By immunizing BALB/c mice with peripheral blood leukocytes (PBL) we have obtained a monoclonal antibody (MAb) named Co.1C9D10, specific for the gamma chain of ruminant fibrinogen. This MAb immunoprecipitates a molecule of apparent molecular weight of 55/65 kDa under reducing conditions and of around 300 kDa under nonreducing conditions. Co.1C9D10 recognized the gamma chain of fibrinogen by immunoblotting techniques and blood vessel plasma from paraffin embedded tissues by immunocytological techniques. Flow cytometry analysis showed that fibrinogen appears adhered to monocytes and granulocytes being eliminated through EDTA treatment of the cells. This phenomenon explains why PBL used as immunogen was able to induce an MAb against fibrinogen.
A monoclonal antibody to an ovine gp130 molecule inhibits homotypic aggregation induced by anti-CD43 monoclonal antibodies of ruminant leukocytes.
A monoclonal antibody (mAb) Co.3E1D4 which recognized a molecule present on the surface of ovine peripheral blood granulocytes, monocytes, platelets and a small percentage of lymphocytes, was able to block leukocyte aggregation induced by mAbs specific for the leukosialin or CD43 molecule. Co.3E1D4 precipitated an antigen with apparent molecular weight of 130 kDa (under reducing and non-reducing conditions). Endoglycosidase F digestion of the molecule resulted in reduction in the apparent molecular weight. These data suggest that the antigen recognized by Co.3E1D4 may be homologous to the human CD31 or platelet endothelial cell adhesion molecule-1 (PECAM-1). Aggregation produced by other inducers such as PMA was not blocked showing that only some cell-cell interactions were mediated by the gp130 molecule.